CRMB_VAR67
ID CRMB_VAR67 Reviewed; 349 AA.
AC P0DSV7; P34015; Q85407; Q89098; Q89118;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2019, sequence version 1.
DT 03-AUG-2022, entry version 8.
DE RecName: Full=Cytokine response-modifying protein B;
DE Short=CrmB;
DE AltName: Full=Viral tumor necrosis factor receptor II;
DE Short=vTNFR;
DE Flags: Precursor;
GN Name=CRMB; ORFNames=G2R, G4R;
OS Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX NCBI_TaxID=587200;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT "Genes of variola and vaccinia viruses necessary to overcome the host
RT protective mechanisms.";
RL FEBS Lett. 319:80-83(1993).
RN [2]
RP FUNCTION, AND INTERACTION WITH HOST TNF; LTA; CCL28; CCL25; CXCL12; CXCL13
RP AND CXCL14.
RX PubMed=16581912; DOI=10.1073/pnas.0510462103;
RA Alejo A., Ruiz-Arguello M.B., Ho Y., Smith V.P., Saraiva M., Alcami A.;
RT "A chemokine-binding domain in the tumor necrosis factor receptor from
RT variola (smallpox) virus.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:5995-6000(2006).
RN [3]
RP FUNCTION.
RX PubMed=17070121; DOI=10.1016/j.bbapap.2006.09.006;
RA Gileva I.P., Nepomnyashchikh T.S., Antonets D.V., Lebedev L.R.,
RA Kochneva G.V., Grazhdantseva A.V., Shchelkunov S.N.;
RT "Properties of the recombinant TNF-binding proteins from variola,
RT monkeypox, and cowpox viruses are different.";
RL Biochim. Biophys. Acta 1764:1710-1718(2006).
RN [4]
RP SUBUNIT.
RX PubMed=19961417; DOI=10.1134/s0006297909120098;
RA Gileva I.P., Nepomnyashchikh T.S., Ryazankin I.A., Shchelkunov S.N.;
RT "Recombinant TNF-binding protein from variola virus as a novel potential
RT TNF antagonist.";
RL Biochemistry (Mosc.) 74:1356-1362(2009).
RN [5]
RP DOMAIN.
RX PubMed=20979600; DOI=10.1186/1756-0500-3-271;
RA Antonets D.V., Nepomnyashchikh T.S., Shchelkunov S.N.;
RT "SECRET domain of variola virus CrmB protein can be a member of poxviral
RT type II chemokine-binding proteins family.";
RL BMC Res. Notes 3:271-271(2010).
CC -!- FUNCTION: Inhibits host immune defense by binding to host TNF and
CC various chemokines (PubMed:16581912, PubMed:17070121). Binds host CC
CC chemokines (beta chemokines) and CXC chemokines (alpha chemokines)
CC (PubMed:16581912). {ECO:0000269|PubMed:16581912,
CC ECO:0000269|PubMed:17070121}.
CC -!- SUBUNIT: Homodimer (PubMed:19961417). Interacts with host TNF, LTA,
CC CCL28, CCL25, CXCL12, CXCL13 and CXCl14 (PubMed:16581912).
CC {ECO:0000269|PubMed:16581912, ECO:0000269|PubMed:19961417}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- DOMAIN: The N-terminal region is similar to host TNF receptor
CC 2/TNFRSF1B and binds host TNF. The C-terminal region is called smallpox
CC virus-encoded chemokine receptor (SECRET), and binds host cytokines
CC (PubMed:20979600). {ECO:0000269|PubMed:20979600}.
CC -!- SIMILARITY: Belongs to the poxviridae chemokine-binding protein family.
CC {ECO:0000305}.
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DR EMBL; X69198; CAA49137.1; -; Genomic_DNA.
DR EMBL; X67117; CAA47540.1; -; Genomic_DNA.
DR PIR; D36858; D36858.
DR RefSeq; NP_042240.1; NC_001611.1.
DR SMR; P0DSV7; -.
DR DIP; DIP-61146N; -.
DR IntAct; P0DSV7; 11.
DR GeneID; 1486427; -.
DR KEGG; vg:1486427; -.
DR Proteomes; UP000002060; Genome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005031; F:tumor necrosis factor receptor activity; IEA:InterPro.
DR GO; GO:0039553; P:suppression by virus of host chemokine activity; IEA:UniProtKB-KW.
DR CDD; cd15839; TNFRSF_viral; 1.
DR InterPro; IPR010806; Poxvirus_TNF-rcpt-II_C.
DR InterPro; IPR011172; Poxvirus_TNF_rcpt-II.
DR InterPro; IPR001368; TNFR/NGFR_Cys_rich_reg.
DR InterPro; IPR034059; TNFRSF_N_viral.
DR Pfam; PF07190; DUF1406; 1.
DR Pfam; PF00020; TNFR_c6; 1.
DR PIRSF; PIRSF001790; TNF_C22L; 1.
DR SMART; SM00208; TNFR; 3.
DR PROSITE; PS00652; TNFR_NGFR_1; 2.
DR PROSITE; PS50050; TNFR_NGFR_2; 2.
PE 1: Evidence at protein level;
KW Disulfide bond; Glycoprotein; Host-virus interaction;
KW Inhibition of host chemokines by virus; Reference proteome; Repeat;
KW Secreted; Signal; Viral immunoevasion.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..349
FT /note="Cytokine response-modifying protein B"
FT /id="PRO_0000034615"
FT REPEAT 31..66
FT /note="TNFR-Cys 1"
FT REPEAT 67..108
FT /note="TNFR-Cys 2"
FT REGION 1..160
FT /note="TNF-binding"
FT REGION 161..349
FT /note="Chemokine-binding"
FT CARBOHYD 101
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 173
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 189
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 215
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 248
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT DISULFID 32..43
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00206"
FT DISULFID 44..57
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00206"
FT DISULFID 47..65
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00206"
FT DISULFID 68..83
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00206"
FT DISULFID 86..100
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00206"
FT DISULFID 90..108
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00206"
SQ SEQUENCE 349 AA; 38189 MW; D45D40B5C6E780EF CRC64;
MKSVLYLYIL FLSCIIINGR DAAPYTPPNG KCKDTEYKRH NLCCLSCPPG TYASRLCDSK
TNTQCTPCGS GTFTSRNNHL PACLSCNGRC NSNQVETRSC NTTHNRICEC SPGYYCLLKG
SSGCKACVSQ TKCGIGYGVS GHTSVGDVIC SPCGFGTYSH TVSSADKCEP VPNNTFNYID
VEITLYPVND TSCTRTTTTG LSESILTSEL TITMNHTDCN PVFREEYFSV LNKVATSGFF
TGENRYQNIS KVCTLNFEIK CNNKGSSFKQ LTKAKNDDGM MSHSETVTLA GDCLSSVDIY
ILYSNTNAQD YETDTISYRV GNVLDDDSHM PGSCNIHKPI TNSKPTRFL
