CRMB_VARV
ID CRMB_VARV Reviewed; 349 AA.
AC P0DSV8; P34015; Q85407; Q89098; Q89118;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2019, sequence version 1.
DT 03-AUG-2022, entry version 12.
DE RecName: Full=Cytokine response-modifying protein B;
DE Short=CrmB;
DE AltName: Full=Viral tumor necrosis factor receptor II;
DE Short=vTNFR;
DE Flags: Precursor;
GN Name=CRMB; ORFNames=G2R, G4R;
OS Variola virus.
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX NCBI_TaxID=10255;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Bangladesh-1975;
RX PubMed=8264798; DOI=10.1038/366748a0;
RA Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C.,
RA Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A.,
RA Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C.;
RT "Potential virulence determinants in terminal regions of variola smallpox
RT virus genome.";
RL Nature 366:748-751(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Garcia-1966, and Somalia-1977;
RA Massung R.F., Loparev V.N., Knight J.C., Chizhikov V.E., Parsons J.M.,
RA Totmenin A.V., Shchelkunov S.N., Esposito J.J.;
RL Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Garcia-1966;
RX PubMed=10639322; DOI=10.1006/viro.1999.0086;
RA Shchelkunov S.N., Totmenin A.V., Loparev V.N., Safronov P.F., Gutorov V.V.,
RA Chizhikov V.E., Knight J.C., Parsons J.M., Massung R.F., Esposito J.J.;
RT "Alastrim smallpox variola minor virus genome DNA sequences.";
RL Virology 266:361-386(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Butler-1952, Chimp 9-4, Garcia-1966, and Somalia-1977;
RA Loparev V.N., Parsons J.M., Esposito J.J.;
RT "DNA sequence analysis as a criterion for allocation of the orthopoxviruses
RT to a particular species.";
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Inhibits host immune defense by binding to host TNF and
CC various chemokines (By similarity). Binds host CC chemokines (beta
CC chemokines) and CXC chemokines (alpha chemokines) (By similarity).
CC {ECO:0000250|UniProtKB:P0DSV7}.
CC -!- SUBUNIT: Homodimer. Interacts with host TNF, LTA, CCL28, CCL25, CXCL12,
CC CXCL13 and CXCl14. {ECO:0000250|UniProtKB:P0DSV7}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P0DSV7}.
CC -!- DOMAIN: The N-terminal region is similar to host TNF receptor
CC 2/TNFRSF1B and binds host TNF. The C-terminal region is called smallpox
CC virus-encoded chemokine receptor (SECRET), and binds host cytokines.
CC {ECO:0000250|UniProtKB:P0DSV7}.
CC -!- SIMILARITY: Belongs to the poxviridae chemokine-binding protein family.
CC {ECO:0000305}.
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DR EMBL; L22579; AAA60933.1; -; Genomic_DNA.
DR EMBL; U18339; AAA69407.1; -; Genomic_DNA.
DR EMBL; U18341; AAA69467.1; -; Genomic_DNA.
DR EMBL; Y16780; CAB54798.1; -; Genomic_DNA.
DR EMBL; U88146; AAB94371.1; -; Genomic_DNA.
DR EMBL; U88148; AAB94373.1; -; Genomic_DNA.
DR EMBL; U88149; AAB94374.1; -; Genomic_DNA.
DR EMBL; U88152; AAB94377.1; -; Genomic_DNA.
DR PIR; D36858; D36858.
DR PIR; T28623; T28623.
DR RefSeq; NP_042240.1; NC_001611.1.
DR SMR; P0DSV8; -.
DR DIP; DIP-61146N; -.
DR GeneID; 1486427; -.
DR KEGG; vg:1486427; -.
DR Proteomes; UP000111493; Genome.
DR Proteomes; UP000119805; Genome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005031; F:tumor necrosis factor receptor activity; IEA:InterPro.
DR GO; GO:0039553; P:suppression by virus of host chemokine activity; IEA:UniProtKB-KW.
DR CDD; cd15839; TNFRSF_viral; 1.
DR InterPro; IPR010806; Poxvirus_TNF-rcpt-II_C.
DR InterPro; IPR011172; Poxvirus_TNF_rcpt-II.
DR InterPro; IPR001368; TNFR/NGFR_Cys_rich_reg.
DR InterPro; IPR034059; TNFRSF_N_viral.
DR Pfam; PF07190; DUF1406; 1.
DR Pfam; PF00020; TNFR_c6; 1.
DR PIRSF; PIRSF001790; TNF_C22L; 1.
DR SMART; SM00208; TNFR; 3.
DR PROSITE; PS00652; TNFR_NGFR_1; 2.
DR PROSITE; PS50050; TNFR_NGFR_2; 2.
PE 3: Inferred from homology;
KW Disulfide bond; Glycoprotein; Host-virus interaction;
KW Inhibition of host chemokines by virus; Repeat; Secreted; Signal;
KW Viral immunoevasion.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..349
FT /note="Cytokine response-modifying protein B"
FT /id="PRO_0000448128"
FT REPEAT 31..66
FT /note="TNFR-Cys 1"
FT REPEAT 67..108
FT /note="TNFR-Cys 2"
FT REGION 1..160
FT /note="TNF-binding"
FT REGION 161..349
FT /note="Chemokine-binding"
FT CARBOHYD 101
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 173
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 189
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 215
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 248
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT DISULFID 32..43
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00206"
FT DISULFID 44..57
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00206"
FT DISULFID 47..65
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00206"
FT DISULFID 68..83
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00206"
FT DISULFID 86..100
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00206"
FT DISULFID 90..108
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00206"
FT VARIANT 17
FT /note="Missing (in strain: Bangladesh-1975 and Chimp 9-4)"
FT VARIANT 160
FT /note="H -> Y (in strain: Butler-1952, Garcia-1966 and
FT Somalia-1977)"
FT VARIANT 165
FT /note="A -> T (in strain: Butler-1952 and Garcia-1966)"
FT VARIANT 182
FT /note="E -> K (in strain: Somalia-1977)"
FT VARIANT 274
FT /note="A -> E (in strain: Somalia-1977)"
FT VARIANT 335
FT /note="N -> D (in strain: Butler-1952 and Garcia-1966)"
FT VARIANT 339
FT /note="P -> L (in strain: Butler-1952 and Garcia-1966)"
SQ SEQUENCE 349 AA; 38189 MW; D45D40B5C6E780EF CRC64;
MKSVLYLYIL FLSCIIINGR DAAPYTPPNG KCKDTEYKRH NLCCLSCPPG TYASRLCDSK
TNTQCTPCGS GTFTSRNNHL PACLSCNGRC NSNQVETRSC NTTHNRICEC SPGYYCLLKG
SSGCKACVSQ TKCGIGYGVS GHTSVGDVIC SPCGFGTYSH TVSSADKCEP VPNNTFNYID
VEITLYPVND TSCTRTTTTG LSESILTSEL TITMNHTDCN PVFREEYFSV LNKVATSGFF
TGENRYQNIS KVCTLNFEIK CNNKGSSFKQ LTKAKNDDGM MSHSETVTLA GDCLSSVDIY
ILYSNTNAQD YETDTISYRV GNVLDDDSHM PGSCNIHKPI TNSKPTRFL
