CRM_DROME
ID CRM_DROME Reviewed; 982 AA.
AC O76906; Q0KHW5; Q8MM10; Q8MM41; Q8MM71; Q8MX89; Q8MX90; Q8MX91; Q8MX92;
AC Q8MX93; Q8MX94; Q8MX95; Q9W4V7;
DT 15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Protein cramped;
GN Name=crm; ORFNames=CG2714;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Oregon-R; TISSUE=Embryo;
RX PubMed=9310333; DOI=10.1242/dev.124.17.3385;
RA Yamamoto Y., Girard F., Bello B., Affolter M., Gehring W.J.;
RT "The cramped gene of Drosophila is a member of the polycomb-group, and
RT interacts with mus209, the gene encoding proliferating cell nuclear
RT antigen.";
RL Development 124:3385-3394(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=k1, k11, k2, k3, k5, k8, k9, pe1, pe10, pe12, pe2, pe29, pe33, pe4,
RC pe45, pe46, pe7, pe9, wi1, wi10, wi12, wi13, wi14, wi16, wi18, wi19, wi2,
RC wi27, wi28, wi4, wi5, wi7, and wi8;
RX PubMed=12351680; DOI=10.1073/pnas.202336899;
RA Harr B., Kauer M., Schloetterer C.;
RT "Hitchhiking mapping: a population-based fine-mapping strategy for adaptive
RT mutations in Drosophilamelanogaster.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:12949-12954(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-431 AND SER-437, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Polycomb group (Pc-G) genes are needed to maintain expression
CC patterns of the homeotic selector genes of the Antennapedia (Antp-C)
CC and Bithorax (Bx-C) complexes, and hence for the maintenance of
CC segmental determination. Can act as a modifier of position effect
CC variegation (PEV). {ECO:0000269|PubMed:9310333}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:9310333}. Note=During
CC S-phase in early embryogenesis. Colocalizes with PCNA in polytene
CC nuclei during embryogenesis.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed throughout embryonic
CC development. High expression is detected in CNS and gonads.
CC {ECO:0000269|PubMed:9310333}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC {ECO:0000269|PubMed:9310333}.
CC -!- SIMILARITY: Belongs to the cramped family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB55550.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Y13674; CAB55550.1; ALT_FRAME; mRNA.
DR EMBL; AF365364; AAN04260.1; -; Genomic_DNA.
DR EMBL; AF365365; AAN04261.1; -; Genomic_DNA.
DR EMBL; AF365366; AAN04262.1; -; Genomic_DNA.
DR EMBL; AF365367; AAN04263.1; -; Genomic_DNA.
DR EMBL; AF365368; AAN04264.1; -; Genomic_DNA.
DR EMBL; AF365369; AAN04265.1; -; Genomic_DNA.
DR EMBL; AF365370; AAN04266.1; -; Genomic_DNA.
DR EMBL; AF365371; AAN04267.1; -; Genomic_DNA.
DR EMBL; AF365372; AAN04268.1; -; Genomic_DNA.
DR EMBL; AF365373; AAN04269.1; -; Genomic_DNA.
DR EMBL; AF365374; AAN04270.1; -; Genomic_DNA.
DR EMBL; AF365375; AAN04271.1; -; Genomic_DNA.
DR EMBL; AF365376; AAN04272.1; -; Genomic_DNA.
DR EMBL; AF365377; AAN04273.1; -; Genomic_DNA.
DR EMBL; AF365378; AAN04274.1; -; Genomic_DNA.
DR EMBL; AF365379; AAN04275.1; -; Genomic_DNA.
DR EMBL; AF365380; AAN04276.1; -; Genomic_DNA.
DR EMBL; AF365381; AAN04277.1; -; Genomic_DNA.
DR EMBL; AF365382; AAN04278.1; -; Genomic_DNA.
DR EMBL; AF365383; AAN04279.1; -; Genomic_DNA.
DR EMBL; AF365384; AAN04280.1; -; Genomic_DNA.
DR EMBL; AF365385; AAN04281.1; -; Genomic_DNA.
DR EMBL; AF365386; AAN04282.1; -; Genomic_DNA.
DR EMBL; AF365387; AAN04283.1; -; Genomic_DNA.
DR EMBL; AF365388; AAN04284.1; -; Genomic_DNA.
DR EMBL; AF365389; AAN04285.1; -; Genomic_DNA.
DR EMBL; AF365390; AAN04286.1; -; Genomic_DNA.
DR EMBL; AF365391; AAN04287.1; -; Genomic_DNA.
DR EMBL; AF365392; AAN04288.1; -; Genomic_DNA.
DR EMBL; AF365393; AAN04289.1; -; Genomic_DNA.
DR EMBL; AF365394; AAN04290.1; -; Genomic_DNA.
DR EMBL; AF365396; AAN04291.1; -; Genomic_DNA.
DR EMBL; AF365397; AAN04292.1; -; Genomic_DNA.
DR EMBL; AF365398; AAN04293.1; -; Genomic_DNA.
DR EMBL; AE014298; AAG22402.1; -; Genomic_DNA.
DR EMBL; AL021728; CAA16818.1; -; Genomic_DNA.
DR EMBL; AY051767; AAK93191.1; -; mRNA.
DR PIR; T13653; T13653.
DR RefSeq; NP_477105.2; NM_057757.4.
DR RefSeq; NP_726829.1; NM_166951.2.
DR AlphaFoldDB; O76906; -.
DR SMR; O76906; -.
DR BioGRID; 57799; 11.
DR IntAct; O76906; 6.
DR STRING; 7227.FBpp0070472; -.
DR iPTMnet; O76906; -.
DR PaxDb; O76906; -.
DR PRIDE; O76906; -.
DR EnsemblMetazoa; FBtr0070495; FBpp0070472; FBgn0000376.
DR EnsemblMetazoa; FBtr0070496; FBpp0070473; FBgn0000376.
DR GeneID; 31268; -.
DR KEGG; dme:Dmel_CG2714; -.
DR CTD; 109545; -.
DR FlyBase; FBgn0000376; crm.
DR VEuPathDB; VectorBase:FBgn0000376; -.
DR eggNOG; KOG4468; Eukaryota.
DR GeneTree; ENSGT00390000003337; -.
DR HOGENOM; CLU_311066_0_0_1; -.
DR InParanoid; O76906; -.
DR PhylomeDB; O76906; -.
DR BioGRID-ORCS; 31268; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31268; -.
DR PRO; PR:O76906; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0000376; Expressed in cleaving embryo and 21 other tissues.
DR Genevisible; O76906; DM.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; NAS:UniProtKB.
DR GO; GO:0007389; P:pattern specification process; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; NAS:UniProtKB.
DR GO; GO:0007379; P:segment specification; IMP:UniProtKB.
DR CDD; cd00167; SANT; 1.
DR InterPro; IPR001005; SANT/Myb.
DR SMART; SM00717; SANT; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..982
FT /note="Protein cramped"
FT /id="PRO_0000197138"
FT DOMAIN 109..173
FT /note="SANT"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 71..111
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 323..349
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 407..456
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 822..851
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..24
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 86..100
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 411..425
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 823..850
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 431
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 437
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VARIANT 322
FT /note="S -> N (in strain: k3 and k5)"
FT VARIANT 402
FT /note="M -> I (in strain: k5)"
FT VARIANT 493
FT /note="N -> T (in strain: Berkeley, k1, k8, Oregon-R, pe7
FT and pe10)"
FT VARIANT 527
FT /note="S -> T (in strain: k2)"
FT VARIANT 639
FT /note="G -> A (in strain: Berkeley, k1, k2, k3, k5, k8, k9
FT and k11)"
FT VARIANT 747
FT /note="M -> I (in strain: k5)"
FT VARIANT 823
FT /note="T -> I (in strain: Berkeley, k9, wi10 and wi19)"
FT VARIANT 830
FT /note="T -> S (in strain: k1)"
FT VARIANT 837
FT /note="C -> S (in strain: Berkeley, k3, k8, k9, wi10 and
FT wi19)"
FT VARIANT 842
FT /note="M -> T (in strain: Berkeley, k1, k2, k3, k5, k8, k9,
FT k11, pe1, pe4, pe45, pe5, wi4, wi7, wi10, wi13, wi18 and
FT wi19)"
FT VARIANT 845
FT /note="S -> P (in strain: k8)"
FT CONFLICT 645
FT /note="A -> S (in Ref. 1; CAB55550, 2; AAN04260/AAN04261/
FT AAN04262/AAN04263/AAN04264/AAN04265/AAN04266/AAN04267/
FT AAN04268/AAN04269/AAN04270/AAN04271/AAN04272/AAN04273/
FT AAN04274/AAN04275/AAN04276/AAN04277/AAN04278/AAN04279/
FT AAN04280/AAN04281/AAN04282/AAN04283/AAN04284/AAN04285/
FT AAN04286/AAN04287/AAN04288/AAN04289/AAN04290/AAN04291/
FT AAN04292/AAN04293 and 5; CAA16818)"
FT /evidence="ECO:0000305"
FT CONFLICT 653
FT /note="G -> S (in Ref. 1; CAB55550, 2; AAN04260/AAN04261/
FT AAN04262/AAN04263/AAN04264/AAN04265/AAN04266/AAN04267/
FT AAN04268/AAN04269/AAN04270/AAN04271/AAN04272/AAN04273/
FT AAN04274/AAN04275/AAN04276/AAN04277/AAN04278/AAN04279/
FT AAN04280/AAN04281/AAN04282/AAN04283/AAN04284/AAN04285/
FT AAN04286/AAN04287/AAN04288/AAN04289/AAN04290/AAN04291/
FT AAN04292/AAN04293 and 5; CAA16818)"
FT /evidence="ECO:0000305"
FT CONFLICT 965
FT /note="F -> L (in Ref. 1; CAB55550)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 982 AA; 107416 MW; 37DC1820DB3ABBA3 CRC64;
MEELSKQPPP PPLTQPPPPS SSVSIEEPLP NGKGGGAVVV NSIAKLPEEE LLGSVTMHNC
PGTRASARVI QKMKQDQTRP MTPPPSEREP NKKEEKAAQK TPSQLKTGSG KTTWTNVERN
CFFDALNEFG KDFEAVANCI NAKLKRRNAN SDYSFKTKDQ VRQHYYQTHH KICKYVRFSE
ELKKPAQELY TLINYGEMRR KLQFLTEKHF MKLKQLVYQG QITVRCKGKN IRIKTPSCKA
LRRLNQLDDS LEDIRLPSKV EVLVTPANME AFGRVQSLAQ NPRGRIIVPL HKKLISFIKT
FEYKWRSANQ RLHEEKSAYF SSSLPSAASN NNNNNNETEP LQPSVASLDP SMCFQPRPGV
AIHRPLLSIT AYLSSISICL TAYEERMGFK VRSETLGNLA GMSVAANKRL RTESGSEKRS
PETKKPKSSA SPPLEKSLDD GPLEGNLMKM ENSSGDELGE EIHEFLGDIL EAMQHPQAAT
IPALSATTGD TTNVAVALET SHDPVQQAYP ANADLSNAMA TSVLQTSCAA APAPSTPVTG
SLAAPSVARS KRKEAKEAAA AAQARNFKPL LSDDILKRIR KGWTQANAAD ITIGDLYVVF
GQDSKLELEY YWCEVDSSTA MASSILTINT VTPSSSSVGT QTGSAASNAN QTGASSNCYV
SASSNSSTSS TSLPYNPNDC DSVERVRAVT TSSVSNKLKH LLLVANLSER VRKRQCNCGH
TCDRKRDLMT KAQQLAEATA TGGVGGMVEG NFRTPMLPVR RPISNIDPVR QLSALTRQKI
SRQVLVQRRL LPPTSAGDRP YDLLSVRQLH SGLFEPIDRV DGTSSAGIST SGSKPDCSMN
AMTASQDQEP GDQGALEFLN DEATQVSARD MPNLDICVAT NRTDVSSSLN EAAQDGSTTQ
SFFQGSMSPM HLLRDSTSNA RWLEDNINDF SLTSLLGHLD EIDATRDILD PSSSMSVISE
SSVDFRHKFQ EIAALLQQQE KD
