ID   CRN15_PHYIN             Reviewed;         615 AA.
AC   Q2M3Z8;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   21-FEB-2006, sequence version 1.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=Crinkler effector protein 15 {ECO:0000303|PubMed:16380277};
DE   Flags: Precursor;
GN   Name=CRN15 {ECO:0000303|PubMed:16380277};
OS   Phytophthora infestans (Potato late blight agent) (Botrytis infestans).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=4787;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Isolate 88069;
RX   PubMed=16380277; DOI=10.1016/j.fgb.2005.10.003;
RA   Win J., Kanneganti T.D., Torto-Alalibo T., Kamoun S.;
RT   "Computational and comparative analyses of 150 full-length cDNA sequences
RT   from the oomycete plant pathogen Phytophthora infestans.";
RL   Fungal Genet. Biol. 43:20-33(2006).
RN   [2]
RP   DOMAIN, AND SUBCELLULAR LOCATION.
RX   PubMed=20847293; DOI=10.1073/pnas.1008491107;
RA   Schornack S., van Damme M., Bozkurt T.O., Cano L.M., Smoker M., Thines M.,
RA   Gaulin E., Kamoun S., Huitema E.;
RT   "Ancient class of translocated oomycete effectors targets the host
RT   nucleus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:17421-17426(2010).
CC   -!- FUNCTION: Secreted effector that elicits necrosis in host plants, a
CC       characteristic of plant innate immunity. {ECO:0000305|PubMed:20847293}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20847293}. Host
CC       nucleus {ECO:0000269|PubMed:20847293}.
CC   -!- DOMAIN: The CRN proteins have modular architectures that include a
CC       signal peptide, a conserved N-terminus, and highly diverse C-terminal
CC       domains. The conserved CRN N-terminus harbors a distinct LXLFLAK motif,
CC       which is followed by the conserved DWL domain. A highly conserved
CC       HVLVXXP motif marks the end of the CRN N-terminal domains and forms a
CC       junction where diverse C-terminal domains are fused. The conserved CRN
CC       N-terminus mediates the translocation into the plant host cells.
CC       {ECO:0000269|PubMed:20847293}.
CC   -!- SIMILARITY: Belongs to the Crinkler effector family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY961463; AAY43409.1; -; mRNA.
DR   AlphaFoldDB; Q2M3Z8; -.
DR   VEuPathDB; FungiDB:PITG_14309; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   InterPro; IPR045379; Crinkler_N.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF20147; Crinkler; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Host nucleus; Secreted; Signal; Virulence.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   CHAIN           18..615
FT                   /note="Crinkler effector protein 15"
FT                   /id="PRO_0000447410"
FT   REGION          18..54
FT                   /note="LQLFLAK domain"
FT                   /evidence="ECO:0000305|PubMed:20847293"
FT   REGION          55..136
FT                   /note="DWL domain"
FT                   /evidence="ECO:0000305|PubMed:20847293"
FT   MOTIF           137..143
FT                   /note="HVLVXXP motif"
FT                   /evidence="ECO:0000305|PubMed:20847293"
FT   CARBOHYD        531
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   615 AA;  69062 MW;  133A2824D80AC3F2 CRC64;
     MVKLVCAIVG VAGSAFPVDI DASQLVGDLK KAIKAENAMT FTGDAKDLQL FLAKQPVDDE
     SGKEVVPVYR PSAEEMKEES FKWLPDEHRA ALKLVEGESD DYIHALTAGE PILGSKTLTT
     WFYTKNNMEL PSSEQIHVLV VVPEQGSSVP TVSQDGVFDH CINPFFLQFR TVDKVGDWLE
     FSSLLPLTRR QKLYIRSSYQ VIANHALFNP NVGMVKYAVV TGTPGVGKSV FVYYVLWRLI
     KEKKRVLLFD NNGLFYFDGS TMLICLALPS KFNEQFWSPD LWCLVDSMDP TSIPGLPYRL
     CSVLLASTPR RDCIGEFKKQ PPTADVFYMP LWSKEELATI APMYPHAAAV WENRFDCLGG
     VPRLVLQDIE TDPQALLMSA CSSCSLDDCI MLVSIYSEIN SKTKIVQTLI HIHSQEPYRK
     YKVVYASDLA MQLIVRTKWR FDRAKLQSLL GSSDGNPLAQ SLCGYIFEFY SMDRLEQGGT
     FVYRELFSGK RKRTPADGTI DIPRSSQPRQ VAERVEVGQH AKQLYVPGTS NYTAIDAWMP
     QFGGFQMTVG KTHDIKGGAA DDLAKLGQNG NRLFFLLPPL YYKTFTKKTP QTIKQYAILV
     PYPEVRNELS ASTLQ