CRN4_CAEEL
ID CRN4_CAEEL Reviewed; 298 AA.
AC Q10905;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Cell death-related nuclease 4;
DE EC=3.1.-.-;
GN Name=crn-4; ORFNames=AH9.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=12718884; DOI=10.1016/s1097-2765(03)00095-9;
RA Parrish J.Z., Xue D.;
RT "Functional genomic analysis of apoptotic DNA degradation in C. elegans.";
RL Mol. Cell 11:987-996(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Involved in the degradation of chromosomal DNA. Contributes
CC to cell killing. {ECO:0000269|PubMed:12718884}.
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DR EMBL; AY303578; AAP57300.1; -; mRNA.
DR EMBL; FO080097; CCD61184.1; -; Genomic_DNA.
DR PIR; T30086; T30086.
DR RefSeq; NP_508415.1; NM_076014.3.
DR PDB; 3CG7; X-ray; 2.50 A; A/B=1-298.
DR PDB; 3CM5; X-ray; 2.81 A; A/B=1-298.
DR PDB; 3CM6; X-ray; 2.60 A; A/B=1-298.
DR PDB; 5DK5; X-ray; 2.10 A; A/B=1-298.
DR PDBsum; 3CG7; -.
DR PDBsum; 3CM5; -.
DR PDBsum; 3CM6; -.
DR PDBsum; 5DK5; -.
DR AlphaFoldDB; Q10905; -.
DR SMR; Q10905; -.
DR BioGRID; 45481; 7.
DR STRING; 6239.AH9.2; -.
DR ChEMBL; CHEMBL3879829; -.
DR EPD; Q10905; -.
DR PaxDb; Q10905; -.
DR PeptideAtlas; Q10905; -.
DR EnsemblMetazoa; AH9.2.1; AH9.2.1; WBGene00000797.
DR GeneID; 180536; -.
DR KEGG; cel:CELE_AH9.2; -.
DR UCSC; AH9.2; c. elegans.
DR CTD; 180536; -.
DR WormBase; AH9.2; CE03858; WBGene00000797; crn-4.
DR eggNOG; KOG0542; Eukaryota.
DR GeneTree; ENSGT00530000063205; -.
DR HOGENOM; CLU_037266_4_3_1; -.
DR InParanoid; Q10905; -.
DR OMA; QDLWRIA; -.
DR OrthoDB; 809000at2759; -.
DR PhylomeDB; Q10905; -.
DR EvolutionaryTrace; Q10905; -.
DR PRO; PR:Q10905; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00000797; Expressed in embryo and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IDA:WormBase.
DR GO; GO:0000175; F:3'-5'-exoribonuclease activity; IBA:GO_Central.
DR GO; GO:0004536; F:deoxyribonuclease activity; IDA:WormBase.
DR GO; GO:0004520; F:endodeoxyribonuclease activity; IDA:WormBase.
DR GO; GO:0004540; F:ribonuclease activity; IDA:WormBase.
DR GO; GO:0003697; F:single-stranded DNA binding; IDA:WormBase.
DR GO; GO:0006309; P:apoptotic DNA fragmentation; IDA:WormBase.
DR GO; GO:0000737; P:DNA catabolic process, endonucleolytic; IDA:WormBase.
DR GO; GO:0000738; P:DNA catabolic process, exonucleolytic; IDA:WormBase.
DR GO; GO:0000467; P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0006401; P:RNA catabolic process; IDA:WormBase.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Apoptosis; Hydrolase; Nuclease; Reference proteome.
FT CHAIN 1..298
FT /note="Cell death-related nuclease 4"
FT /id="PRO_0000021003"
FT DOMAIN 12..192
FT /note="Exonuclease"
FT /evidence="ECO:0000255"
FT STRAND 9..18
FT /evidence="ECO:0007829|PDB:5DK5"
FT STRAND 31..40
FT /evidence="ECO:0007829|PDB:5DK5"
FT TURN 41..44
FT /evidence="ECO:0007829|PDB:5DK5"
FT STRAND 45..56
FT /evidence="ECO:0007829|PDB:5DK5"
FT STRAND 59..61
FT /evidence="ECO:0007829|PDB:5DK5"
FT HELIX 66..72
FT /evidence="ECO:0007829|PDB:5DK5"
FT HELIX 76..80
FT /evidence="ECO:0007829|PDB:5DK5"
FT STRAND 82..84
FT /evidence="ECO:0007829|PDB:3CG7"
FT HELIX 85..98
FT /evidence="ECO:0007829|PDB:5DK5"
FT STRAND 105..113
FT /evidence="ECO:0007829|PDB:5DK5"
FT HELIX 114..117
FT /evidence="ECO:0007829|PDB:5DK5"
FT HELIX 119..127
FT /evidence="ECO:0007829|PDB:5DK5"
FT HELIX 133..135
FT /evidence="ECO:0007829|PDB:5DK5"
FT STRAND 136..140
FT /evidence="ECO:0007829|PDB:5DK5"
FT HELIX 141..147
FT /evidence="ECO:0007829|PDB:5DK5"
FT HELIX 149..152
FT /evidence="ECO:0007829|PDB:5DK5"
FT HELIX 162..169
FT /evidence="ECO:0007829|PDB:5DK5"
FT STRAND 177..180
FT /evidence="ECO:0007829|PDB:5DK5"
FT HELIX 181..197
FT /evidence="ECO:0007829|PDB:5DK5"
FT STRAND 206..210
FT /evidence="ECO:0007829|PDB:5DK5"
FT HELIX 213..215
FT /evidence="ECO:0007829|PDB:5DK5"
FT HELIX 225..227
FT /evidence="ECO:0007829|PDB:5DK5"
FT HELIX 229..239
FT /evidence="ECO:0007829|PDB:5DK5"
FT STRAND 243..246
FT /evidence="ECO:0007829|PDB:5DK5"
FT TURN 249..251
FT /evidence="ECO:0007829|PDB:5DK5"
FT HELIX 254..256
FT /evidence="ECO:0007829|PDB:5DK5"
FT TURN 261..263
FT /evidence="ECO:0007829|PDB:5DK5"
FT TURN 267..271
FT /evidence="ECO:0007829|PDB:5DK5"
FT HELIX 279..282
FT /evidence="ECO:0007829|PDB:5DK5"
FT HELIX 290..295
FT /evidence="ECO:0007829|PDB:5DK5"
SQ SEQUENCE 298 AA; 34645 MW; E9E6DEAD91FCA071 CRC64;
MAYQHCPFDT LLILDFETTS DAANQDYPCE VIQFAIVAYD VPNDKIREDI SFNKYVKPVL
NRTLTKNCVD FTGIPQRSID TADTFDVVYE QFQQWLITLG LEEGKFAFVC DSRQDLWRIA
QYQMKLSNIQ MPAFFRQYIN LYKIFTNEMD RMGPKELSAT TNIGKMNEYY DLPTIGRAHD
AMDDCLNIAT ILQRMINMGA KVTVNELLTC CASWRRQPLV YNKEWRSSFM DAGKIFERVL
PLVVTTIRAG DFRLEMYGVC RYCRKGMDVC GTSHQQTPHD LYKNEEDPIH FAKIAGYY
