ID   CRN4_PHYCP              Reviewed;         426 AA.
AC   P0CV73;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   03-AUG-2022, entry version 8.
DE   RecName: Full=Crinkler effector protein 4 {ECO:0000303|PubMed:26011314};
GN   Name=CRN4 {ECO:0000303|PubMed:26011314};
GN   Synonyms=CRN83_152 {ECO:0000303|PubMed:23536880};
OS   Phytophthora capsici.
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=4784;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], DOMAIN, AND FUNCTION.
RX   PubMed=23536880; DOI=10.1371/journal.pone.0059517;
RA   Stam R., Jupe J., Howden A.J., Morris J.A., Boevink P.C., Hedley P.E.,
RA   Huitema E.;
RT   "Identification and characterisation CRN effectors in Phytophthora capsici
RT   shows modularity and functional diversity.";
RL   PLoS ONE 8:E59517-E59517(2013).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, INDUCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=26011314; DOI=10.1371/journal.pone.0127965;
RA   Mafurah J.J., Ma H., Zhang M., Xu J., He F., Ye T., Shen D., Chen Y.,
RA   Rajput N.A., Dou D.;
RT   "A Virulence Essential CRN Effector of Phytophthora capsici Suppresses Host
RT   Defense and Induces Cell Death in Plant Nucleus.";
RL   PLoS ONE 10:E0127965-E0127965(2015).
CC   -!- FUNCTION: Secreted effector that is critical to pathogenesis by
CC       suppressing plant immune responsess (PubMed:23536880, PubMed:26011314).
CC       Promotes Phytophthora infection by suppressing the H(2)O(2)
CC       accumulation and callose deposition (PubMed:26011314). May induce cell
CC       death by regulating expression of cell death-related genes
CC       (PubMed:26011314). {ECO:0000269|PubMed:23536880,
CC       ECO:0000269|PubMed:26011314}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23536880}. Host
CC       nucleus {ECO:0000269|PubMed:23536880}. Note=The nuclear localization is
CC       required for both of its cell death-inducing activity and virulent
CC       function. {ECO:0000269|PubMed:26011314}.
CC   -!- INDUCTION: Expression is up-regulated at the early stages of infection.
CC       {ECO:0000269|PubMed:26011314}.
CC   -!- DOMAIN: The CRN proteins have modular architectures that include a
CC       signal peptide, a conserved N-terminus, and highly diverse C-terminal
CC       domains. The conserved CRN N-terminus harbors a distinct LXLFLAK motif,
CC       which is followed by the conserved DWL domain. A highly conserved
CC       HVLVXXP motif marks the end of the CRN N-terminal domains and forms a
CC       junction where diverse C-terminal domains are fused. The conserved CRN
CC       N-terminus mediates the translocation into the plant host cells.
CC       {ECO:0000305|PubMed:23536880}.
CC   -!- DISRUPTION PHENOTYPE: Leads to the loss of abilities to suppress
CC       pathogen-associated molecular patterns (PAMPS)-triggered immunity (PTI)
CC       in host cells. {ECO:0000269|PubMed:26011314}.
CC   -!- SIMILARITY: Belongs to the Crinkler effector family. {ECO:0000305}.
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DR   AlphaFoldDB; P0CV73; -.
DR   VEuPathDB; FungiDB:DVH05_003841; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   InterPro; IPR045379; Crinkler_N.
DR   Pfam; PF20147; Crinkler; 1.
PE   2: Evidence at transcript level;
KW   Host nucleus; Secreted; Virulence.
FT   CHAIN           1..426
FT                   /note="Crinkler effector protein 4"
FT                   /id="PRO_0000448090"
FT   REGION          20..57
FT                   /note="LQLFLAK domain"
FT                   /evidence="ECO:0000305|PubMed:23536880"
FT   REGION          59..107
FT                   /note="DWL domain"
FT                   /evidence="ECO:0000305|PubMed:23536880"
FT   REGION          115..426
FT                   /note="Effector domain"
FT                   /evidence="ECO:0000305|PubMed:23536880"
FT   MOTIF           108..114
FT                   /note="HVLVXXP motif"
FT                   /evidence="ECO:0000305|PubMed:23536880"
SQ   SEQUENCE   426 AA;  47002 MW;  3F94275ECD997601 CRC64;
     MVKLSLQCAV VDQAGSSFDV EIDDSAKVSK LKKVIKEENP ATITCDAKDL QLFLAKKDDA
     WLDGAGAAAV ELDEHGHPQG CVQMDPTLWV KNPKHFGDNF QPGEGQVHVL VVVPEGVVGS
     ASETSKMDFV VDKVSKLYEH SVLSKRTRYV HSEMSSSKGN KLVKELKIRV TPVDAVPFTG
     GSPTPVEEFE WIKGRTEEQQ SGRYRDYVEA NIGDVLRNNK LCVFSVEKGA NILSVEVPGC
     DVDLAGRTDM IVLSAIVQKF PHYLPHLPGV KMLIEVKREV KSASEFQALS ELIAMDFIVD
     ESVMALLTNL TNHWEFLWVS NKSNNRPIIA TTTLTTPGEA FEVIRTLLAQ SSTADADIML
     PCLAEPVKRR KLNQMLPFIG EASGDGIRES IERYYDIASC LGPDFDMARA VARQVTRSIP
     TLSYFS