ID   CRN5_PHYIN              Reviewed;         349 AA.
AC   Q2M408;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   21-FEB-2006, sequence version 1.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=Crinkler effector protein 5 {ECO:0000303|PubMed:16380277};
DE   Flags: Precursor;
GN   Name=CRN5 {ECO:0000303|PubMed:16380277};
OS   Phytophthora infestans (Potato late blight agent) (Botrytis infestans).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=4787;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Isolate 88069;
RX   PubMed=16380277; DOI=10.1016/j.fgb.2005.10.003;
RA   Win J., Kanneganti T.D., Torto-Alalibo T., Kamoun S.;
RT   "Computational and comparative analyses of 150 full-length cDNA sequences
RT   from the oomycete plant pathogen Phytophthora infestans.";
RL   Fungal Genet. Biol. 43:20-33(2006).
RN   [2]
RP   DOMAIN, SUBCELLULAR LOCATION, AND FUNCTION.
RX   PubMed=20847293; DOI=10.1073/pnas.1008491107;
RA   Schornack S., van Damme M., Bozkurt T.O., Cano L.M., Smoker M., Thines M.,
RA   Gaulin E., Kamoun S., Huitema E.;
RT   "Ancient class of translocated oomycete effectors targets the host
RT   nucleus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:17421-17426(2010).
CC   -!- FUNCTION: Secreted effector that elicits necrosis in host plants, a
CC       characteristic of plant innate immunity. {ECO:0000269|PubMed:20847293}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20847293}. Host
CC       nucleus {ECO:0000269|PubMed:20847293}.
CC   -!- DOMAIN: The CRN proteins have modular architectures that include a
CC       signal peptide, a conserved N-terminus, and highly diverse C-terminal
CC       domains. The conserved CRN N-terminus harbors a distinct LXLFLAK motif,
CC       which is followed by the conserved DWL domain. A highly conserved
CC       HVLVXXP motif marks the end of the CRN N-terminal domains and forms a
CC       junction where diverse C-terminal domains are fused. The conserved CRN
CC       N-terminus mediates the translocation into the plant host cells.
CC       {ECO:0000269|PubMed:20847293}.
CC   -!- SIMILARITY: Belongs to the Crinkler effector family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY961453; AAY43399.1; -; mRNA.
DR   AlphaFoldDB; Q2M408; -.
DR   VEuPathDB; FungiDB:PITG_12009; -.
DR   VEuPathDB; FungiDB:PITG_22988; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   InterPro; IPR045379; Crinkler_N.
DR   Pfam; PF20147; Crinkler; 1.
PE   2: Evidence at transcript level;
KW   Host nucleus; Secreted; Signal; Virulence.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   CHAIN           18..349
FT                   /note="Crinkler effector protein 5"
FT                   /id="PRO_0000447408"
FT   REGION          18..57
FT                   /note="LQLFLAK domain"
FT                   /evidence="ECO:0000305|PubMed:20847293"
FT   REGION          58..108
FT                   /note="DWL domain"
FT                   /evidence="ECO:0000305|PubMed:20847293"
FT   MOTIF           109..115
FT                   /note="HVLVXXP motif"
FT                   /evidence="ECO:0000305|PubMed:20847293"
SQ   SEQUENCE   349 AA;  39498 MW;  A4073B2D77502035 CRC64;
     MVKLFCSIVG VAGSPFSVEV NEGKTVDDLK KAIKAENLDD PTLRNVAPKN LQLFLAKKGD
     AWLRYNEDLD TYLQSEIDTS SYLHMRASWK LSKPTLFGPD VSLGEDVVHV LVVVPGQRLP
     IAATAIHEPH PARKKRWEEL NKILDRNQRA KVNAAGESST GYSYVSFSDV DRVMRARRYE
     QPRKVIENEK IDVLYEYLLL LTKAFGEIVN GKEAKRLYFI VPVLVCVCGL FDGEVRILAE
     ETVTGKRVHG DGAFEFVIER GSKRVCIVKA KRDDFQQGLA QAYVGSEVLA DLEGLTDLFS
     IVTNFKEWYF SRCLNDRVER DDATMDMEHD IPTREAVKKI AEKIYSMLS