ID   CRNA_EMENI              Reviewed;         507 AA.
AC   P22152; C8VU57; Q5BEM2;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=Nitrate transporter;
DE   AltName: Full=Nitrate permease;
GN   Name=crnA; ORFNames=AN1008;
OS   Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS   M139) (Aspergillus nidulans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=227321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1986367; DOI=10.1073/pnas.88.1.204;
RA   Unkles S.E., Hawker K.L., Grieve C., Campbell E.I., Montague P.,
RA   Kinghorn J.R.;
RT   "crnA encodes a nitrate transporter in Aspergillus nidulans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:204-208(1991).
RN   [2]
RP   ERRATUM OF PUBMED:1986367.
RA   Unkles S.E., Hawker K.L., Grieve C., Campbell E.I., Montague P.,
RA   Kinghorn J.R.;
RL   Proc. Natl. Acad. Sci. U.S.A. 88:4564-4564(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Trueman L.J., Onyeocha I., Forde B.G.;
RT   "Molecular biology of a family of nitrate and nitrite transporters found in
RT   bacteria, fungi and plants.";
RL   Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=16372000; DOI=10.1038/nature04341;
RA   Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA   Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA   Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA   Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA   Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA   Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA   Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA   Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA   Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT   "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT   fumigatus and A. oryzae.";
RL   Nature 438:1105-1115(2005).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA   Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA   Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA   Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA   Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA   Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA   Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA   Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA   van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA   Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA   Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA   Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA   Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA   Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA   van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA   Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA   Oliver S.G., Turner G.;
RT   "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT   effort.";
RL   Fungal Genet. Biol. 46:S2-13(2009).
CC   -!- FUNCTION: Permease for nitrate uptake.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- INDUCTION: Subject to nitrate and nitrite induction, and nitrogen
CC       metabolite repression. CrnA expression is mediated by the products of
CC       nirA, areA, and niaD.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR   EMBL; M61125; AAA62125.1; -; Genomic_DNA.
DR   EMBL; U34382; AAA76713.1; -; mRNA.
DR   EMBL; AACD01000015; EAA65576.1; -; Genomic_DNA.
DR   EMBL; BN001308; CBF88345.1; -; Genomic_DNA.
DR   PIR; A38560; A38560.
DR   RefSeq; XP_658612.1; XM_653520.1.
DR   AlphaFoldDB; P22152; -.
DR   STRING; 162425.CADANIAP00001640; -.
DR   TCDB; 2.A.1.8.5; the major facilitator superfamily (mfs).
DR   EnsemblFungi; CBF88345; CBF88345; ANIA_01008.
DR   EnsemblFungi; EAA65576; EAA65576; AN1008.2.
DR   GeneID; 2876788; -.
DR   KEGG; ani:AN1008.2; -.
DR   VEuPathDB; FungiDB:AN1008; -.
DR   eggNOG; ENOG502QPIC; Eukaryota.
DR   HOGENOM; CLU_024204_1_1_1; -.
DR   InParanoid; P22152; -.
DR   OMA; IPCFMFA; -.
DR   OrthoDB; 542533at2759; -.
DR   Proteomes; UP000000560; Chromosome VIII.
DR   Proteomes; UP000005890; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015513; F:high-affinity secondary active nitrite transmembrane transporter activity; IMP:AspGD.
DR   GO; GO:0015112; F:nitrate transmembrane transporter activity; IDA:AspGD.
DR   GO; GO:0015113; F:nitrite transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0022832; F:voltage-gated channel activity; IDA:AspGD.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   GO; GO:0015706; P:nitrate transmembrane transport; IDA:AspGD.
DR   GO; GO:0015707; P:nitrite transport; IMP:AspGD.
DR   CDD; cd17341; MFS_NRT2_like; 1.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR044772; NO3_transporter.
DR   InterPro; IPR004737; NO3_transporter_NarK/NarU-like.
DR   PANTHER; PTHR23515; PTHR23515; 1.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   TIGRFAMs; TIGR00886; 2A0108; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Nitrate assimilation; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..507
FT                   /note="Nitrate transporter"
FT                   /id="PRO_0000084837"
FT   TOPO_DOM        1..34
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        35..55
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        56..71
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        72..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        93..100
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        101..121
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        122..130
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        131..151
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        152..161
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        162..182
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        183..198
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        199..219
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        220..306
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        307..327
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        328..357
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        358..378
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        379..389
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        390..410
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        411..417
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        418..438
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        439..507
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   507 AA;  54925 MW;  4A3D3FA643F16952 CRC64;
     MDFAKLLVAS PEVNPNNRKA LTIPVLNPFN TYGRVFFFSW FGFMLAFLSW YAFPPLLTVT
     IRDDLDMSQT QIANSNIIAL LATLLVRLIC GPLCDRFGPR LVFIGLLLVG SIPTAMAGLV
     TSPQGLIALR FFIGILGGTF VPCQVWCTGF FDKSIVGTAN SLAAGLGNAG GGITYFVMPA
     IFDSLIRDQG LPAHKAWRVA YIVPFILIVA AALGMLFTCD DTPTGKWSER HIWMKEDTQT
     ASKGNIVDLS SGAQSSRPSG PPSIIAYAIP DVEKKGTETP LEPQSQAIGQ FDAFRANAVA
     SPSRKEAFNV IFSLATMAVA VPYACSFGSE LAINSILGDY YDKNFPYMGQ TQTGKWAAMF
     GFLNIVCRPA GGFLADFLYR KTNTPWAKKL LLSFLGVVMG AFMIAMGFSD PKSEATMFGL
     TAGLAFFLES CNGAIFSLVP HVHPYANGIV SGMVGGFGNL GGIIFAIIFR YSHHDYARGI
     WILGVISMAV FISVSWVRPV PKSQMRE