CRNL1_MOUSE
ID CRNL1_MOUSE Reviewed; 690 AA.
AC P63154; Q542E8; Q9CQC1;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Crooked neck-like protein 1;
DE AltName: Full=Crooked neck homolog;
GN Name=Crnkl1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Embryo, Lung, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH PPIL2 AND HSP90, AND REGION.
RX PubMed=15189447; DOI=10.1111/j.1356-9597.2004.00742.x;
RA Hatakeyama S., Matsumoto M., Yada M., Nakayama K.I.;
RT "Interaction of U-box-type ubiquitin-protein ligases (E3s) with molecular
RT chaperones.";
RL Genes Cells 9:533-548(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-689, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Involved in pre-mRNA splicing process.
CC {ECO:0000250|UniProtKB:Q9BZJ0}.
CC -!- SUBUNIT: Identified in the spliceosome C complex. Present in a
CC spliceosome complex assembled in vitro containing CRNKL1, HPRP8BP and
CC SNRPB2 (By similarity). Interacts with PPIL2 (via the PPIase
CC cyclophilin-type domain); they may form a trimeric complex with HSP90
CC (PubMed:15189447). {ECO:0000250|UniProtKB:Q9BZJ0,
CC ECO:0000269|PubMed:15189447}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9BZJ0}. Nucleus
CC speckle {ECO:0000250|UniProtKB:Q9BZJ0}. Note=Colocalizes with core
CC spliceosomal snRNP proteins. {ECO:0000250|UniProtKB:Q9BZJ0}.
CC -!- SIMILARITY: Belongs to the crooked-neck family. {ECO:0000305}.
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DR EMBL; AK004749; BAB23530.1; -; mRNA.
DR EMBL; AK012962; BAB28572.1; -; mRNA.
DR EMBL; AK088882; BAC40630.1; -; mRNA.
DR EMBL; BC029187; AAH29187.1; -; mRNA.
DR CCDS; CCDS16828.1; -.
DR RefSeq; NP_080096.1; NM_025820.3.
DR AlphaFoldDB; P63154; -.
DR SMR; P63154; -.
DR BioGRID; 211782; 35.
DR IntAct; P63154; 28.
DR MINT; P63154; -.
DR STRING; 10090.ENSMUSP00000001818; -.
DR iPTMnet; P63154; -.
DR PhosphoSitePlus; P63154; -.
DR EPD; P63154; -.
DR MaxQB; P63154; -.
DR PaxDb; P63154; -.
DR PeptideAtlas; P63154; -.
DR PRIDE; P63154; -.
DR ProteomicsDB; 277897; -.
DR Antibodypedia; 9534; 65 antibodies from 13 providers.
DR DNASU; 66877; -.
DR Ensembl; ENSMUST00000001818; ENSMUSP00000001818; ENSMUSG00000001767.
DR GeneID; 66877; -.
DR KEGG; mmu:66877; -.
DR UCSC; uc008mrz.1; mouse.
DR CTD; 51340; -.
DR MGI; MGI:1914127; Crnkl1.
DR VEuPathDB; HostDB:ENSMUSG00000001767; -.
DR eggNOG; KOG1915; Eukaryota.
DR GeneTree; ENSGT00550000074931; -.
DR HOGENOM; CLU_011554_1_0_1; -.
DR InParanoid; P63154; -.
DR OMA; HIKVWIS; -.
DR OrthoDB; 355408at2759; -.
DR PhylomeDB; P63154; -.
DR TreeFam; TF300305; -.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR BioGRID-ORCS; 66877; 26 hits in 73 CRISPR screens.
DR ChiTaRS; Crnkl1; mouse.
DR PRO; PR:P63154; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; P63154; protein.
DR Bgee; ENSMUSG00000001767; Expressed in animal zygote and 251 other tissues.
DR Genevisible; P63154; MM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; ISO:MGI.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0071014; C:post-mRNA release spliceosomal complex; IBA:GO_Central.
DR GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR GO; GO:0005681; C:spliceosomal complex; ISS:UniProtKB.
DR GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0000245; P:spliceosomal complex assembly; ISS:UniProtKB.
DR Gene3D; 1.25.40.10; -; 3.
DR InterPro; IPR003107; HAT.
DR InterPro; IPR045075; Syf1-like.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR11246; PTHR11246; 1.
DR Pfam; PF02184; HAT; 2.
DR SMART; SM00386; HAT; 13.
DR SUPFAM; SSF48452; SSF48452; 2.
PE 1: Evidence at protein level;
KW mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Spliceosome.
FT CHAIN 1..690
FT /note="Crooked neck-like protein 1"
FT /id="PRO_0000205720"
FT REPEAT 61..93
FT /note="HAT 1"
FT REPEAT 95..127
FT /note="HAT 2"
FT REPEAT 129..161
FT /note="HAT 3"
FT REPEAT 163..194
FT /note="HAT 4"
FT REPEAT 196..227
FT /note="HAT 5"
FT REPEAT 229..264
FT /note="HAT 6"
FT REPEAT 266..300
FT /note="HAT 7"
FT REPEAT 310..342
FT /note="HAT 8"
FT REPEAT 344..378
FT /note="HAT 9"
FT REPEAT 388..424
FT /note="HAT 10"
FT REPEAT 459..491
FT /note="HAT 11"
FT REPEAT 493..527
FT /note="HAT 12"
FT REPEAT 529..560
FT /note="HAT 13"
FT REPEAT 565..606
FT /note="HAT 14"
FT REPEAT 608..646
FT /note="HAT 15"
FT REPEAT 648..673
FT /note="HAT 16"
FT REGION 250..467
FT /note="Mediates interaction with HSP90"
FT /evidence="ECO:0000269|PubMed:15189447"
FT REGION 667..690
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 618..626
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOD_RES 342
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BZJ0"
FT MOD_RES 689
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355"
SQ SEQUENCE 690 AA; 83416 MW; 9FE84D7F1205B974 CRC64;
MAASTAAGKQ RIPKVAKVKN KAPAEVQITA EQLLREAKER ELELLPPPPQ QKITDEEELN
DYKLRKRKTF EDNIRKNRTV ISNWIKYAQW EESLKEIQRA RSIYERALDV DYRNITLWLK
YAEMEMKNRQ VNHARNIWDR AITTLPRVNQ FWYKYTYMEE MLGNVAGARQ VFERWMEWQP
EEQAWHSYIN FELRYKEVER ARTIYERFVL VHPAVKNWIK YARFEEKHAY FAHARKVYER
AVEFFGDEHM DEHLYVAFAK FEENQKEFER VRVIYKYALD RISKQEAQEL FKNYTIFEKK
FGDRRGIEDI IVSKRRFQYE EEVKANPHNY DAWFDYLRLV ESDAEADTVR EVYERAIANV
PPIQEKRHWK RYIYLWVNYA LYEELEAKDP ERTRQVYQAS LELIPHKKFT FAKMWLYYAQ
FEIRQKNLPF ARRALGTSIG KCPKNKLFKG YIELELQLRE FDRCRKLYEK FLEFGPENCT
SWIKFAELET ILGDIERARA IYELAISQPR LDMPEVLWKS YIDFEIEQEE TERTRNLYRQ
LLQRTQHVKV WISFAQFELS SGKEGSVAKC RQIYEEANKT MRNCEEKEER LMLLESWRSF
EDEFGTVSDK ERVDKLMPEK VKKRRKVQAD DGSDAGWEEY YDYIFPEDAA NQPNLKLLAM
AKLWKKQQQE REAAEQDPDK DIDESESSSF
