ID   CRN_DROME               Reviewed;         702 AA.
AC   P17886; O46071; Q24283;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2001, sequence version 2.
DT   03-AUG-2022, entry version 188.
DE   RecName: Full=Protein crooked neck;
GN   Name=crn; ORFNames=CG3193;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=2044955; DOI=10.1101/gad.5.6.1080;
RA   Zhang K., Smouse D., Perrimon N.;
RT   "The crooked neck gene of Drosophila contains a motif found in a family of
RT   yeast cell cycle genes.";
RL   Genes Dev. 5:1080-1091(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Oregon-R;
RX   PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA   Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA   Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA   Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA   Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA   Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA   Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA   McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA   Glover D.M.;
RT   "From sequence to chromosome: the tip of the X chromosome of D.
RT   melanogaster.";
RL   Science 287:2220-2222(2000).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Embryo;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [6]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=10502111; DOI=10.1007/s004270050287;
RA   Jack J., Myette G.;
RT   "Mutations that alter the morphology of the malpighian tubules in
RT   Drosophila.";
RL   Dev. Genes Evol. 209:546-554(1999).
RN   [7]
RP   FUNCTION.
RX   PubMed=12163015; DOI=10.1016/s0006-291x(02)00863-x;
RA   Raisin-Tani S., Leopold P.;
RT   "Drosophila crooked-neck protein co-fractionates in a multiprotein complex
RT   with splicing factors.";
RL   Biochem. Biophys. Res. Commun. 296:288-292(2002).
CC   -!- FUNCTION: May be involved in pre-mRNA splicing process
CC       (PubMed:12163015, PubMed:2044955). Involved in embryonic neurogenesis
CC       and cell rearrangement during Malpighian tubule morphogenesis
CC       (PubMed:2044955, PubMed:10502111). {ECO:0000269|PubMed:10502111,
CC       ECO:0000269|PubMed:12163015, ECO:0000269|PubMed:2044955}.
CC   -!- SUBUNIT: Colocalizes with a complex containing snRNP proteins.
CC   -!- SUBCELLULAR LOCATION: Nucleus speckle. Note=Concentrated in nuclear
CC       speckles.
CC   -!- TISSUE SPECIFICITY: Transcribed in all cells during embryonic
CC       development.
CC   -!- DEVELOPMENTAL STAGE: Is expressed throughout embryonic, larval, pupal
CC       and adult stages at relatively constant levels.
CC   -!- DISRUPTION PHENOTYPE: Causes defects in the proliferation of brain
CC       neuroblasts and results in the absence of identified neuronal lineages
CC       in the central and peripheral nervous systems (PubMed:2044955). During
CC       embryonic Malpighian morphogenesis, most of the cells of the Malpighian
CC       tubules fail to undergo rearrangement to form tubes with a two cell
CC       circumference. Only cells at the distal end of the tubule rearrange
CC       (PubMed:10502111). {ECO:0000269|PubMed:10502111,
CC       ECO:0000269|PubMed:2044955}.
CC   -!- SIMILARITY: Belongs to the crooked-neck family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X58374; CAA41263.1; -; mRNA.
DR   EMBL; AE014298; AAF45760.1; -; Genomic_DNA.
DR   EMBL; AL009195; CAA15705.1; -; Genomic_DNA.
DR   EMBL; AY051666; AAK93090.1; -; mRNA.
DR   PIR; T13427; T13427.
DR   RefSeq; NP_477118.1; NM_057770.5.
DR   AlphaFoldDB; P17886; -.
DR   SMR; P17886; -.
DR   BioGRID; 57745; 64.
DR   DIP; DIP-17429N; -.
DR   IntAct; P17886; 21.
DR   MINT; P17886; -.
DR   STRING; 7227.FBpp0070402; -.
DR   PaxDb; P17886; -.
DR   PRIDE; P17886; -.
DR   DNASU; 31208; -.
DR   EnsemblMetazoa; FBtr0070418; FBpp0070402; FBgn0000377.
DR   GeneID; 31208; -.
DR   KEGG; dme:Dmel_CG3193; -.
DR   CTD; 12935; -.
DR   FlyBase; FBgn0000377; crn.
DR   VEuPathDB; VectorBase:FBgn0000377; -.
DR   eggNOG; KOG1915; Eukaryota.
DR   GeneTree; ENSGT00550000074931; -.
DR   HOGENOM; CLU_011554_1_0_1; -.
DR   InParanoid; P17886; -.
DR   OMA; HIKVWIS; -.
DR   OrthoDB; 355408at2759; -.
DR   PhylomeDB; P17886; -.
DR   Reactome; R-DME-72163; mRNA Splicing - Major Pathway.
DR   SignaLink; P17886; -.
DR   BioGRID-ORCS; 31208; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 31208; -.
DR   PRO; PR:P17886; -.
DR   Proteomes; UP000000803; Chromosome X.
DR   Bgee; FBgn0000377; Expressed in eye disc (Drosophila) and 23 other tissues.
DR   Genevisible; P17886; DM.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase.
DR   GO; GO:0005829; C:cytosol; IDA:FlyBase.
DR   GO; GO:0016607; C:nuclear speck; IDA:FlyBase.
DR   GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR   GO; GO:0071014; C:post-mRNA release spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0071011; C:precatalytic spliceosome; HDA:FlyBase.
DR   GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR   GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; ISS:FlyBase.
DR   GO; GO:0008366; P:axon ensheathment; IMP:FlyBase.
DR   GO; GO:0008347; P:glial cell migration; IMP:FlyBase.
DR   GO; GO:0007443; P:Malpighian tubule morphogenesis; IMP:FlyBase.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   GO; GO:0007438; P:oenocyte development; IMP:FlyBase.
DR   GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IMP:FlyBase.
DR   GO; GO:0000245; P:spliceosomal complex assembly; IBA:GO_Central.
DR   Gene3D; 1.25.40.10; -; 3.
DR   InterPro; IPR003107; HAT.
DR   InterPro; IPR045075; Syf1-like.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   PANTHER; PTHR11246; PTHR11246; 1.
DR   Pfam; PF02184; HAT; 2.
DR   SMART; SM00386; HAT; 14.
DR   SUPFAM; SSF48452; SSF48452; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; Differentiation; mRNA processing; mRNA splicing;
KW   Neurogenesis; Nucleus; Reference proteome; Repeat.
FT   CHAIN           1..702
FT                   /note="Protein crooked neck"
FT                   /id="PRO_0000205718"
FT   REPEAT          56..88
FT                   /note="HAT 1"
FT   REPEAT          90..122
FT                   /note="HAT 2"
FT   REPEAT          124..156
FT                   /note="HAT 3"
FT   REPEAT          158..189
FT                   /note="HAT 4"
FT   REPEAT          191..222
FT                   /note="HAT 5"
FT   REPEAT          224..259
FT                   /note="HAT 6"
FT   REPEAT          261..295
FT                   /note="HAT 7"
FT   REPEAT          305..337
FT                   /note="HAT 8"
FT   REPEAT          339..373
FT                   /note="HAT 9"
FT   REPEAT          383..419
FT                   /note="HAT 10"
FT   REPEAT          454..486
FT                   /note="HAT 11"
FT   REPEAT          488..522
FT                   /note="HAT 12"
FT   REPEAT          524..555
FT                   /note="HAT 13"
FT   REGION          670..702
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           620..628
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        94
FT                   /note="R -> P (in Ref. 1; CAA41263)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   702 AA;  84262 MW;  8E03A869B84E4A12 CRC64;
     MERPQKMPKV AKVKNKAPAE VQITAEQLLR EAKERDLEIL PPPPKQKISD PAELADYQQR
     KRKTFEDNLR KNRMVVSHWI KYAQWEEQQQ EIQRARSIWE RALDNEHRNV TLWLKYAEME
     MKNKQVNHAR NLWDRAVTIM PRVNQFWYKY TYMEEMLENV AGARQVFERW MEWQPEEQAW
     QTYVNFELRY KEIDRAREIY ERFVYVHPDV KNWIKFARFE ESHGFIHGSR RVFERAVEFF
     GDDYIEERLF IAFARFEEGQ KEHDRARIIY KYALDHLPKD RTQELFKAYT KHEKKYGDRA
     GIEDVIVSKR KYQYEQEVAA NPTNYDAWFD YLRLIEAEGD RDQIRETYER AISNVPPANE
     KNFWRRYIYL WINYALYEEL EAEDAERTRQ IYKTCLELIP HKQFTFSKLW LLYAQFEIRC
     KELQRARKAL GLAIGMCPRD KLFRGYIDLE IQLREFERCR MLYEKFLEFG PENCVTWMKF
     AELENLLGDT DRARAIFELA VQQPRLDMPE LLWKAYIDFE VALGETELAR QLYERLLERT
     QHVKVWMSFA KFEMGLSHGD SGPDAELNVQ LARRIYERAN EMLRQLGDKE SRVLLLEAWR
     DFERDASDSQ EMQKVMDKMP RRIKKRQKIV SDNGVEEGWE EVFDYIFPED EMARPNLKLL
     AAAKMWKTQK DNTVDDPPAT AIASEPEPAA DAAPADTTDS GD