CROCC_CAEEL
ID CROCC_CAEEL Reviewed; 1955 AA.
AC Q09EF7; G4SGS2; G4SGT1; G4SGT2; Q09EF5; Q09EF6; Q8MQ60;
DT 18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 2.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Rootletin {ECO:0000250|UniProtKB:Q5TZA2};
DE AltName: Full=Abnormal dye filling protein 14 {ECO:0000305|PubMed:17314406};
DE AltName: Full=Chemotaxis abnormal protein 10 {ECO:0000312|WormBase:F35D11.11b};
GN Name=che-10 {ECO:0000303|PubMed:2428682, ECO:0000312|WormBase:F35D11.11b};
GN Synonyms=dyf-14 {ECO:0000303|PubMed:17314406,
GN ECO:0000312|WormBase:F35D11.11b};
GN ORFNames=F35D11.11 {ECO:0000312|WormBase:F35D11.11b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=2428682; DOI=10.1016/0012-1606(86)90314-3;
RA Perkins L.A., Hedgecock E.M., Thomson J.N., Culotti J.G.;
RT "Mutant sensory cilia in the nematode Caenorhabditis elegans.";
RL Dev. Biol. 117:456-487(1986).
RN [3] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17314406; DOI=10.1091/mbc.e06-09-0805;
RA Ou G., Koga M., Blacque O.E., Murayama T., Ohshima Y., Schafer J.C., Li C.,
RA Yoder B.K., Leroux M.R., Scholey J.M.;
RT "Sensory ciliogenesis in Caenorhabditis elegans: assignment of IFT
RT components into distinct modules based on transport and phenotypic
RT profiles.";
RL Mol. Biol. Cell 18:1554-1569(2007).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=24094853; DOI=10.1016/j.cub.2013.08.033;
RA Mohan S., Timbers T.A., Kennedy J., Blacque O.E., Leroux M.R.;
RT "Striated rootlet and nonfilamentous forms of rootletin maintain ciliary
RT function.";
RL Curr. Biol. 23:2016-2022(2013).
RN [5] {ECO:0000305}
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=27623382; DOI=10.1016/j.devcel.2016.07.013;
RA Nechipurenko I.V., Olivier-Mason A., Kazatskaya A., Kennedy J.,
RA McLachlan I.G., Heiman M.G., Blacque O.E., Sengupta P.;
RT "A Conserved role for girdin in basal body positioning and ciliogenesis.";
RL Dev. Cell 38:493-506(2016).
CC -!- FUNCTION: Major structural component of the ciliary rootlet, a
CC cytoskeletal-like structure in ciliated cells which originates from the
CC basal body at the proximal end of a cilium and extends proximally
CC toward the cell nucleus (PubMed:24094853). Required for cilia integrity
CC and function in sensory neurons (PubMed:2428682, PubMed:17314406,
CC PubMed:24094853). Maintains cilia integrity, partly by modulating the
CC assembly and transport of intraflagellar proteins along the ciliary
CC axoneme (PubMed:24094853). Required for normal mating behavior and
CC normal responses to environmental and chemical stimuli (PubMed:2428682,
CC PubMed:17314406, PubMed:24094853). {ECO:0000269|PubMed:17314406,
CC ECO:0000269|PubMed:24094853, ECO:0000269|PubMed:2428682}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium basal body
CC {ECO:0000269|PubMed:24094853, ECO:0000269|PubMed:27623382}. Cytoplasm,
CC cytoskeleton, cilium axoneme {ECO:0000269|PubMed:24094853}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=b {ECO:0000312|WormBase:F35D11.11b};
CC IsoId=Q09EF7-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:F35D11.11a};
CC IsoId=Q09EF7-2; Sequence=VSP_058714, VSP_058715;
CC Name=c {ECO:0000312|WormBase:F35D11.11c};
CC IsoId=Q09EF7-3; Sequence=VSP_058716, VSP_058717;
CC Name=d {ECO:0000312|WormBase:F35D11.11d};
CC IsoId=Q09EF7-4; Sequence=VSP_058713;
CC -!- TISSUE SPECIFICITY: Expressed in head ciliated neurons.
CC {ECO:0000269|PubMed:27623382}.
CC -!- DISRUPTION PHENOTYPE: Ciliary degeneration which is partly due to
CC irregular localization of ciliary components (PubMed:24094853). This
CC likely leads to the impaired intraflagellar transport of proteins along
CC the ciliary axoneme during the later stages of larval development
CC (PubMed:24094853). Lack of or defects in amphid cilia including no
CC transition zone and axoneme structures in amphid wing neurons,
CC enlargened dendritic endings, variable cilia length of ADL neurons,
CC absent or irregularly orientated cilia of the AFD neuron and absent
CC striated rootlets which are normally associated with cilia basal bodies
CC and positioned at the base of cilia in IL1, OLQ and BAG neurons
CC (PubMed:2428682, PubMed:17314406, PubMed:24094853). Behavioral defects
CC including no obvious mating behavior, impaired dauer formation, osmotic
CC and CO(2+) avoidance and impaired chemotaxis (PubMed:2428682,
CC PubMed:24094853). {ECO:0000269|PubMed:17314406,
CC ECO:0000269|PubMed:24094853, ECO:0000269|PubMed:2428682}.
CC -!- SIMILARITY: Belongs to the rootletin family. {ECO:0000305}.
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DR EMBL; BX284602; CCD70538.1; -; Genomic_DNA.
DR EMBL; BX284602; CCD70539.1; -; Genomic_DNA.
DR EMBL; BX284602; CCD70540.1; -; Genomic_DNA.
DR EMBL; BX284602; CCD70541.1; -; Genomic_DNA.
DR RefSeq; NP_494819.3; NM_062418.6. [Q09EF7-1]
DR RefSeq; NP_494820.3; NM_062419.5. [Q09EF7-3]
DR RefSeq; NP_494821.3; NM_062420.3. [Q09EF7-2]
DR RefSeq; NP_872028.2; NM_182228.4.
DR AlphaFoldDB; Q09EF7; -.
DR SMR; Q09EF7; -.
DR STRING; 6239.F35D11.11b; -.
DR EPD; Q09EF7; -.
DR PaxDb; Q09EF7; -.
DR EnsemblMetazoa; F35D11.11a.1; F35D11.11a.1; WBGene00018051. [Q09EF7-2]
DR EnsemblMetazoa; F35D11.11b.1; F35D11.11b.1; WBGene00018051. [Q09EF7-1]
DR EnsemblMetazoa; F35D11.11c.1; F35D11.11c.1; WBGene00018051. [Q09EF7-3]
DR EnsemblMetazoa; F35D11.11d.1; F35D11.11d.1; WBGene00018051. [Q09EF7-4]
DR GeneID; 173801; -.
DR KEGG; cel:CELE_F35D11.11; -.
DR UCSC; F35D11.11b; c. elegans.
DR CTD; 173801; -.
DR WormBase; F35D11.11a; CE40410; WBGene00018051; che-10. [Q09EF7-2]
DR WormBase; F35D11.11b; CE40411; WBGene00018051; che-10. [Q09EF7-1]
DR WormBase; F35D11.11c; CE40412; WBGene00018051; che-10. [Q09EF7-3]
DR WormBase; F35D11.11d; CE43383; WBGene00018051; che-10. [Q09EF7-4]
DR eggNOG; ENOG502RT7C; Eukaryota.
DR InParanoid; Q09EF7; -.
DR OMA; AFRMAVN; -.
DR OrthoDB; 63368at2759; -.
DR PhylomeDB; Q09EF7; -.
DR PRO; PR:Q09EF7; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00018051; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005813; C:centrosome; ISS:UniProtKB.
DR GO; GO:0036064; C:ciliary basal body; IDA:UniProtKB.
DR GO; GO:0035869; C:ciliary transition zone; IDA:WormBase.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0060271; P:cilium assembly; IMP:WormBase.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell projection; Cilium;
KW Cilium biogenesis/degradation; Coiled coil; Cytoplasm; Cytoskeleton;
KW Reference proteome.
FT CHAIN 1..1955
FT /note="Rootletin"
FT /evidence="ECO:0000305"
FT /id="PRO_0000438691"
FT REGION 321..341
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 391..451
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 504..551
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 907..935
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 961..998
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 29..58
FT /evidence="ECO:0000255"
FT COILED 162..223
FT /evidence="ECO:0000255"
FT COILED 284..1303
FT /evidence="ECO:0000255"
FT COILED 1368..1579
FT /evidence="ECO:0000255"
FT COILED 1607..1863
FT /evidence="ECO:0000255"
FT COMPBIAS 327..341
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..380
FT /note="Missing (in isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_058713"
FT VAR_SEQ 1884
FT /note="H -> V (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_058714"
FT VAR_SEQ 1885..1955
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_058715"
FT VAR_SEQ 1938..1942
FT /note="DGREA -> GTLIM (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_058716"
FT VAR_SEQ 1943..1955
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_058717"
SQ SEQUENCE 1955 AA; 229626 MW; 712B07AD99A7C5F2 CRC64;
MKSAASSEDV VRVTEDLSEC RNRLDAGIEE NRRNRQVIQD INDQLQRFRQ RANAESIESF
NLTPSPDVTL SSLAHPGLTH LHNQTNISMP SLTIDIPLNS NAMISSSRTP NYAINGLRNR
HKSLVGHRYR STSPIGDYGR HRSSPRVLAH YNLDGADIGV GEENLDELFA KLKEELFKNN
TLEEVNEMLR EENDAALAAN EHLRVDATNL SRQLQQLQQQ QHTESMRFRS ENTRYRNQTE
TQHRKLISLW KEFTAVKRQL HELRTTTAND LDRQLTEFTR CATLMRKAIR HAEQKNLDQK
EQMKREKDDV LDETLRQLNS VTENYMKSEE KANERQRDLK RKEDECRKLR EQNDELSDIL
EQLSKMAHEM AGGRGRNETP MDVARKMRKL LTTKNGEIDE SREAAKQAEK ERDRAKKDLE
KEEKRRKDDR EAERKRSSVY SQREHDLKKL DDELRKASEK IRNLEEQRES QEKLTISVQN
SLNEAHRQHK QFIEELMIRH REELKEREDS HEEALRSKDT EERSRFEKER SEREKIRRES
DELRETQRSL KGDVAAMKTD LDDKTLRLDM LETERDELKK KLETEREQAD QRDLEIAECR
AKLDEMAEKE AELRKELAEF QAIITAMEGE GKLNQEQFLE SKNELNTLTD QIESLNSEVE
NKNEEIRNLM ATLQEKEVHI QNVRTSSHQL TATYEEANGE IDILKAELTR LHEQVNERTR
QISEANEKYD DAARKNDALL EDVATWQEKY EQLKMELEEM NRRGQEKERE EADLRALLDD
LRGNFDKLTN ELKQKGVTVD SLNEEISSLK EQLNKSEKER KEELLRMEEL EQKNEAEMKE
EYEVKLQLAE KDRQGVENFG KECEARMNEL TKIHEMLMEE HDQLKVDHLH TEEEVERLKE
KMRKELEKLN EQNDGDRAEW SNERNRLESS KNEAVTELQE RVQKLEDVVK EKEDKEIALR
RDLEDSHEKS RDLDDKLRKM ELTDEEKEED RKKEQKTLNE ERMKLMEQKE EAMLVATKHA
TTIDQQTRRI SVLEGDVEKL TAGIAERESS INALESNTME LISKLETTEA ELEKLKDELA
VMLKQNSELK NGKEGLSEKW NEERKKIQDL ADQLREANKV VHNMRMKNVN LEEKKNELDQ
NVTDLTNKVR QLEIQLMDKA AKNEVSGDLL RKMEHDAQSM LKQAQNEQFR LTDLEKVRKA
LQDENQRLVN DLATVKAAFE VKRETSKSAI SDILDKYRSA EEKANKGELD NQRLRSDLAT
VTLKLERQEL KAKDSDNRLR DSQKRFEEVQ SKLANLQKSA VESLQNPMSS NSRQNRSIYV
DIPRAASSIG LNENSDEVPL RSSPSVRFAD SSQNMQRAVD SMDVSSSVGV TLRFLKERIE
QLEADNADLS DALEKAKDEL RQRNEKLADR QMVIERVERQ LVHITEERNT IENRMTSQRQ
MYLTNEESSR SREHEIRSMK ARISTLELHL REKESKLAHL RKEIEVLHGQ LHDALESKEK
ATGLVGVQDS KHRDLEEQLD RANRERELAI GKQRRTLAEN ENLFRKLEQL EKEREQLMRE
ITDERRLNER NRTSLEELRV SERTWKSAMT TAKKPAEEQE RAVQEQRRWE ESNHEMTNRN
TALTKECDRL RVEMRDQLNR MNGINLRSVD FERKNEELSS KLIVMQNTVT AMKKFEEEWK
RLEAEMRAEL KILRKEKLMQ TAEIEDLKRK SFRSDTEKKE IEGIRVRLER EISALKRHVD
ALEEEKGKTE KAVRETMNER RAIDKSLASM ERENQQLYRN CAQLQAQIQN LERDAGNRSV
TKLAKEHSLL EARIAALIEE KRQLQSMLDQ KDANYSHKRK LLESQIQLLR EQLEAERRKR
TKGVVATGPT VSRRGVQHTS AFRHTIERHR SLSQSSERTI LQERYLEYVY TGDRTPAIQM
INTPPISPLS HSGSFNSDGR EARIRRESDN GHLQH
