ID   2SS_CUCMO               Reviewed;          61 AA.
AC   P84576;
DT   05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 21.
DE   RecName: Full=2S seed storage albumin protein {ECO:0000305};
DE   AltName: Full=2S albumin {ECO:0000305};
DE   Contains:
DE     RecName: Full=2S albumin small chain;
DE     AltName: Full=Moschin A;
DE   Contains:
DE     RecName: Full=2S albumin large chain;
DE     AltName: Full=Moschin B;
DE   Flags: Fragments;
OS   Cucurbita moschata (Winter crookneck squash) (Cucurbita pepo var.
OS   moschata).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Cucurbiteae; Cucurbita.
OX   NCBI_TaxID=3662;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, AND FUNCTION.
RC   TISSUE=Seed {ECO:0000269|PubMed:12356464};
RX   PubMed=12356464; DOI=10.1016/s1046-5928(02)00500-4;
RA   Ng T.B., Parkash A., Tso W.W.;
RT   "Purification and characterization of moschins, arginine-glutamate-rich
RT   proteins with translation-inhibiting activity from brown pumpkin (Cucurbita
RT   moschata) seeds.";
RL   Protein Expr. Purif. 26:9-13(2002).
CC   -!- FUNCTION: This is a 2S seed storage protein. Inhibits cell-free protein
CC       synthesis. {ECO:0000269|PubMed:12356464, ECO:0000305}.
CC   -!- SUBUNIT: The mature protein consists of a small and a large chain
CC       linked by 2 disulfide bonds. {ECO:0000305}.
CC   -!- MISCELLANEOUS: IC(50) of translation-inhibiting activity is 17 uM
CC       (small chain) and 300 nM (large chain). {ECO:0000269|PubMed:12356464}.
CC   -!- SIMILARITY: Belongs to the 2S seed storage albumins family.
CC       {ECO:0000255}.
CC   -!- CAUTION: Cysteine residues characteristic for this protein family are
CC       absent. {ECO:0000305}.
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DR   AlphaFoldDB; P84576; -.
DR   PRIDE; P84576; -.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protein synthesis inhibitor;
KW   Seed storage protein; Storage protein.
FT   CHAIN           1..>31
FT                   /note="2S albumin small chain"
FT                   /evidence="ECO:0000269|PubMed:12356464"
FT                   /id="PRO_0000032149"
FT   CHAIN           32..>61
FT                   /note="2S albumin large chain"
FT                   /evidence="ECO:0000269|PubMed:12356464"
FT                   /id="PRO_0000032150"
FT   NON_CONS        31..32
FT                   /evidence="ECO:0000305"
FT   NON_TER         61
FT                   /evidence="ECO:0000303|PubMed:12356464"
SQ   SEQUENCE   61 AA;  7720 MW;  A9C4F7F38B78474C CRC64;
     ERRAERESQM RADEEFGSQV QYLTQQRRAR LPWRREGRSF DEEFRELRNV DEEERRDMMI
     E