CRQ_DROME
ID CRQ_DROME Reviewed; 491 AA.
AC Q27367; A4UZX7; Q8SZG1; Q9VPQ1;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2017, sequence version 3.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Protein croquemort;
DE AltName: Full=d-CD36;
GN Name=crq; ORFNames=CG4280;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=Oregon-R;
RX PubMed=8630729; DOI=10.1016/s1074-7613(00)80410-0;
RA Franc N.C., Dimarcq J.-L., Lagueux M., Hoffmann J., Ezekowitz R.A.B.;
RT "Croquemort, a novel Drosophila hemocyte/macrophage receptor that
RT recognizes apoptotic cells.";
RL Immunity 4:431-443(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL48526.1, ECO:0000312|EMBL:AFC17503.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAL48526.1};
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M., Celniker S.,
RA Booth B.;
RL Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-104 AND ASN-110, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=Oregon-R; TISSUE=Head;
RX PubMed=17893096; DOI=10.1093/glycob/cwm097;
RA Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L.,
RA Panin V.;
RT "Identification of N-glycosylated proteins from the central nervous system
RT of Drosophila melanogaster.";
RL Glycobiology 17:1388-1403(2007).
RN [6]
RP DEVELOPMENTAL STAGE.
RX PubMed=21948708; DOI=10.1387/ijdb.103254lh;
RA Herboso L., Talamillo A., Perez C., Barrio R.;
RT "Expression of the Scavenger Receptor Class B type I (SR-BI) family in
RT Drosophila melanogaster.";
RL Int. J. Dev. Biol. 55:603-611(2011).
CC -!- FUNCTION: Macrophage receptor for apoptotic cells.
CC {ECO:0000269|PubMed:8630729}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Found on hemocytes from hemolymph, and later in the
CC development, on macrophages that contain apoptotic cell corpses.
CC {ECO:0000269|PubMed:8630729}.
CC -!- DEVELOPMENTAL STAGE: Expressed on hemocytes/macrophages in
CC embryogenesis (PubMed:8630729). First detected in late stage 11
CC embryos, which corresponds to the first wave of apoptosis
CC (PubMed:8630729). Expressed in cells that by stage 12 spread throughout
CC the embryo and by stage 13/14 have migrated from both ends towards the
CC middle until they are evenly distributed by stage 15 (PubMed:8630729).
CC In L3 instar larvae, expression increases with development in the
CC gastric caeca, midgut, Garland cells and anterior testis
CC (PubMed:21948708). In the ovary expression appears to be restricted to
CC one pole (PubMed:21948708). Expressed in the ring gland, and weak
CC expression in the salivary glands and the central nervous system
CC (PubMed:21948708). {ECO:0000269|PubMed:21948708,
CC ECO:0000269|PubMed:8630729}.
CC -!- PTM: N-glycosylated and/or O-glycosylated.
CC {ECO:0000305|PubMed:17893096}.
CC -!- MISCELLANEOUS: 'Croquemort' means literally 'the one who bites dead
CC persons' in French and is colloquial for undertaker.
CC -!- SIMILARITY: Belongs to the CD36 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA83454.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA83455.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z31583; CAA83455.1; ALT_FRAME; mRNA.
DR EMBL; Z31582; CAA83454.1; ALT_FRAME; mRNA.
DR EMBL; AE014134; AAF51494.1; -; Genomic_DNA.
DR EMBL; AE014134; AAN10497.2; -; Genomic_DNA.
DR EMBL; AE014134; AFH03500.1; -; Genomic_DNA.
DR EMBL; AE014134; AGB92361.1; -; Genomic_DNA.
DR EMBL; AE014134; AGB92362.1; -; Genomic_DNA.
DR EMBL; AY070904; AAL48526.1; -; mRNA.
DR EMBL; BT133259; AFC17503.1; -; mRNA.
DR PIR; S43137; S43137.
DR RefSeq; NP_001245823.1; NM_001258894.1.
DR RefSeq; NP_001259824.1; NM_001272895.1.
DR RefSeq; NP_001259825.1; NM_001272896.1.
DR RefSeq; NP_787957.1; NM_175943.2.
DR RefSeq; NP_787958.1; NM_175944.3.
DR AlphaFoldDB; Q27367; -.
DR SMR; Q27367; -.
DR BioGRID; 59475; 2.
DR IntAct; Q27367; 1.
DR STRING; 7227.FBpp0077747; -.
DR GlyGen; Q27367; 9 sites.
DR iPTMnet; Q27367; -.
DR PaxDb; Q27367; -.
DR EnsemblMetazoa; FBtr0078087; FBpp0077747; FBgn0015924.
DR EnsemblMetazoa; FBtr0078088; FBpp0077748; FBgn0015924.
DR EnsemblMetazoa; FBtr0304741; FBpp0293283; FBgn0015924.
DR EnsemblMetazoa; FBtr0331232; FBpp0303674; FBgn0015924.
DR EnsemblMetazoa; FBtr0331233; FBpp0303675; FBgn0015924.
DR GeneID; 33219; -.
DR KEGG; dme:Dmel_CG4280; -.
DR UCSC; CG4280-RA; d. melanogaster.
DR CTD; 33219; -.
DR FlyBase; FBgn0015924; crq.
DR VEuPathDB; VectorBase:FBgn0015924; -.
DR eggNOG; KOG3776; Eukaryota.
DR GeneTree; ENSGT00940000153372; -.
DR HOGENOM; CLU_019853_5_2_1; -.
DR InParanoid; Q27367; -.
DR OMA; VEMGPYT; -.
DR OrthoDB; 1106566at2759; -.
DR PhylomeDB; Q27367; -.
DR Reactome; R-DME-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-DME-8856828; Clathrin-mediated endocytosis.
DR BioGRID-ORCS; 33219; 1 hit in 1 CRISPR screen.
DR ChiTaRS; crq; fly.
DR GenomeRNAi; 33219; -.
DR PRO; PR:Q27367; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0015924; Expressed in motile cell and 52 other tissues.
DR Genevisible; Q27367; DM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IDA:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005044; F:scavenger receptor activity; ISS:FlyBase.
DR GO; GO:0043277; P:apoptotic cell clearance; IMP:FlyBase.
DR GO; GO:0006915; P:apoptotic process; IMP:FlyBase.
DR GO; GO:0048102; P:autophagic cell death; TAS:FlyBase.
DR GO; GO:0042832; P:defense response to protozoan; IMP:FlyBase.
DR GO; GO:0043652; P:engulfment of apoptotic cell; IDA:FlyBase.
DR GO; GO:0002433; P:immune response-regulating cell surface receptor signaling pathway involved in phagocytosis; IDA:FlyBase.
DR GO; GO:0042116; P:macrophage activation; TAS:FlyBase.
DR GO; GO:0006909; P:phagocytosis; IMP:FlyBase.
DR GO; GO:0006911; P:phagocytosis, engulfment; TAS:FlyBase.
DR GO; GO:0090382; P:phagosome maturation; IMP:FlyBase.
DR GO; GO:0050764; P:regulation of phagocytosis; TAS:FlyBase.
DR InterPro; IPR002159; CD36_fam.
DR PANTHER; PTHR11923; PTHR11923; 1.
DR Pfam; PF01130; CD36; 1.
DR PRINTS; PR01609; CD36FAMILY.
PE 1: Evidence at protein level;
KW Apoptosis; Cell membrane; Disulfide bond; Glycoprotein; Lipoprotein;
KW Membrane; Palmitate; Receptor; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..491
FT /note="Protein croquemort"
FT /id="PRO_0000144164"
FT TOPO_DOM 1..12
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 34..451
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 452..472
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 473..491
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT LIPID 2
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT LIPID 3
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT LIPID 5
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT LIPID 6
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT CARBOHYD 72
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 104
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17893096"
FT CARBOHYD 110
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17893096"
FT CARBOHYD 129
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 189
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 198
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 216
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 260
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 446
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 256..324
FT /evidence="ECO:0000250"
FT DISULFID 285..345
FT /evidence="ECO:0000250"
FT DISULFID 326..334
FT /evidence="ECO:0000250"
FT CONFLICT 247
FT /note="S -> A (in Ref. 1; CAA83455/CAA83454)"
FT /evidence="ECO:0000305"
FT CONFLICT 257
FT /note="G -> S (in Ref. 4; AAL48526)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 491 AA; 56154 MW; 7136F28653281706 CRC64;
MCCKCCGETQ RKVWVFGLGS VFLLLGILIV VFWPGIADNL VEDGLTLKPG TDAYESWLEA
PIPIYLSFYM FNWTNPEDIR NPDIKPNFVE MGPYTFLEKH KKENYTFYDN ATVAYYERRT
WFFDPERSNG TLDDMVTAAH AITATVADEM RDQRKIVKKI INFMLNHEGG KLYVTKPVGE
WIFEGYQDNI TDFLNLFNTT KIDIPYKRFG WLADRNESLT YDGLFTIHTG TDDISNLGRL
THWNGKSETG FYEMPCGIVN GTTGDMFPPK MNVNDEITIF ATDACRFMNL RPRGTYENHG
LTATKWVGTE ETLDSGENYP NQACFCDEAR FDECPKTGVV ECKACRDKAP IYSSFPHFYL
ADQSYVDAVS GMKPEKEKHE FFLAVEPITG VPVQVHGRIQ INMMIEPDDD FDIYRGVQKV
LMPMFWFDQY AELSSELASK AKLAINLSSY GIIFGYSMIA FASVFLITGI TLTVTKKWVR
RTPEDEDILT N
