2SS_FAGES
ID 2SS_FAGES Reviewed; 149 AA.
AC Q2PS07;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 25-MAY-2022, entry version 47.
DE RecName: Full=2S seed storage albumin protein {ECO:0000305};
DE AltName: Full=16 kDa buckwheat protein {ECO:0000303|PubMed:12372998, ECO:0000303|PubMed:20054125, ECO:0000303|PubMed:20407269};
DE Short=BWp16 {ECO:0000303|PubMed:16549935, ECO:0000303|PubMed:18520034, ECO:0000303|PubMed:20054125, ECO:0000303|PubMed:20407269};
DE AltName: Full=2S albumin {ECO:0000305};
DE AltName: Full=2S seed storage protein {ECO:0000305};
DE AltName: Full=Buckwheat 16 kDa major allergen {ECO:0000303|PubMed:16549935, ECO:0000303|PubMed:18520034, ECO:0000303|PubMed:20054125, ECO:0000303|PubMed:20407269};
DE AltName: Allergen=Fag e 2.0101 {ECO:0000305};
DE Flags: Precursor;
OS Fagopyrum esculentum (Common buckwheat) (Polygonum fagopyrum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Polygonaceae; Polygonoideae; Fagopyreae; Fagopyrum.
OX NCBI_TaxID=3617 {ECO:0000312|EMBL:ABC18306.1};
RN [1] {ECO:0000312|EMBL:ABC18306.1}
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND ALLERGEN.
RC TISSUE=Seed {ECO:0000303|PubMed:16549935};
RX PubMed=16549935; DOI=10.1159/000092038;
RA Koyano S., Takagi K., Teshima R., Sawada J.;
RT "Molecular cloning of cDNA, recombinant protein expression and
RT characterization of a buckwheat 16-kDa major allergen.";
RL Int. Arch. Allergy Immunol. 140:73-81(2006).
RN [2] {ECO:0000312|EMBL:ACJ48243.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Wang X., Wu Y., Liu Z.;
RT "Cloning and prokaryotic expression vector construction of common buckwheat
RT 16 kDa major allergen gene.";
RL Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PROTEIN SEQUENCE OF 23-37, AND ALLERGEN.
RX PubMed=12372998; DOI=10.1159/000065173;
RA Tanaka K., Matsumoto K., Akasawa A., Nakajima T., Nagasu T., Iikura Y.,
RA Saito H.;
RT "Pepsin-resistant 16-kD buckwheat protein is associated with immediate
RT hypersensitivity reaction in patients with buckwheat allergy.";
RL Int. Arch. Allergy Immunol. 129:49-56(2002).
RN [4]
RP ALLERGEN, TISSUE SPECIFICITY, BIOTECHNOLOGY, AND MUTAGENESIS OF CYS-38;
RP CYS-52; CYS-77; CYS-83; CYS-87; CYS-88; CYS-98; CYS-100; CYS-133 AND
RP CYS-140.
RX PubMed=18520034; DOI=10.1248/bpb.31.1079;
RA Satoh R., Koyano S., Takagi K., Nakamura R., Teshima R., Sawada J.;
RT "Immunological characterization and mutational analysis of the recombinant
RT protein BWp16, a major allergen in buckwheat.";
RL Biol. Pharm. Bull. 31:1079-1085(2008).
RN [5]
RP CRYSTALLIZATION OF 35-149, ALLERGEN, AND MUTAGENESIS OF 1-MET--MET-34.
RX PubMed=20054125; DOI=10.1107/s1744309109043127;
RA Kezuka Y., Itagaki T., Satoh R., Teshima R., Nonaka T.;
RT "Purification, crystallization and preliminary X-ray analysis of a deletion
RT mutant of a major buckwheat allergen.";
RL Acta Crystallogr. F 65:1267-1270(2009).
RN [6]
RP ALLERGEN, BIOTECHNOLOGY, REGION, SITE, AND MUTAGENESIS OF LYS-120; GLU-121;
RP GLY-122; VAL-123; ARG-124; ASP-125; LEU-126; LYS-127 AND GLU-128.
RX PubMed=20407269; DOI=10.1159/000312630;
RA Satoh R., Koyano S., Takagi K., Nakamura R., Teshima R.;
RT "Identification of an IgE-binding epitope of a major buckwheat allergen,
RT BWp16, by SPOTs assay and mimotope screening.";
RL Int. Arch. Allergy Immunol. 153:133-140(2010).
RN [7]
RP ALLERGEN, DEVELOPMENTAL STAGE, AND BIOTECHNOLOGY.
RX PubMed=21461893; DOI=10.1007/s00403-011-1142-z;
RA Tohgi K., Kohno K., Takahashi H., Matsuo H., Nakayama S., Morita E.;
RT "Usability of Fag e 2 ImmunoCAP in the diagnosis of buckwheat allergy.";
RL Arch. Dermatol. Res. 303:635-642(2011).
CC -!- FUNCTION: Seed storage protein. {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in seeds (at protein level)
CC (PubMed:16549935, PubMed:18520034). {ECO:0000269|PubMed:16549935,
CC ECO:0000269|PubMed:18520034}.
CC -!- DEVELOPMENTAL STAGE: Expressed in shoots 10 days after seeding.
CC {ECO:0000269|PubMed:21461893}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE of
CC patients allergic to Japanese buckwheat (PubMed:16549935,
CC PubMed:12372998, PubMed:18520034, PubMed:20054125, PubMed:20407269,
CC PubMed:21461893). Binds to IgE in 90% of the 10 patients tested having
CC immediate hypersensitivity reactions (IRH) including anaphylaxis, but
CC not to IgE from other 10 patients tested without IHR (PubMed:12372998).
CC Natural protein binds to IgE in 76% of the 17 patients tested
CC (PubMed:16549935). Recombinant protein binds to IgE in 83% of the 29
CC Japanese patients tested (PubMed:18520034). IgE-binding is unaffected
CC by pepsin digestion (PubMed:12372998). {ECO:0000269|PubMed:12372998,
CC ECO:0000269|PubMed:16549935, ECO:0000269|PubMed:18520034,
CC ECO:0000269|PubMed:20054125, ECO:0000269|PubMed:20407269,
CC ECO:0000269|PubMed:21461893}.
CC -!- BIOTECHNOLOGY: This protein may be helpful in engineering hypoallergens
CC for buckwheat allergy (PubMed:18520034, PubMed:20407269). Determining
CC IgE antibody titer of the natural protein in the blood serum using the
CC ImmunoCAP method could be a reliable way to diagnose buckwheat allergy
CC with a sensitivity of 90% and a specificity of 89.6% using a cut-off
CC value of 2.74 kUA/L (PubMed:21461893). {ECO:0000305|PubMed:18520034,
CC ECO:0000305|PubMed:20407269, ECO:0000305|PubMed:21461893}.
CC -!- SIMILARITY: Belongs to the 2S seed storage albumins family.
CC {ECO:0000305}.
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DR EMBL; DQ304682; ABC18306.1; -; mRNA.
DR EMBL; FJ430161; ACJ48243.1; -; mRNA.
DR AlphaFoldDB; Q2PS07; -.
DR Allergome; 1187; Fag e 2.
DR Allergome; 8711; Fag e 2.0101.
DR GO; GO:0043245; C:extraorganismal space; IDA:UniProtKB.
DR GO; GO:0019863; F:IgE binding; IEA:UniProtKB-KW.
DR GO; GO:0045735; F:nutrient reservoir activity; IC:UniProtKB.
DR GO; GO:0048367; P:shoot system development; IEP:UniProtKB.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR000617; Napin/2SS/CON.
DR PANTHER; PTHR35496; PTHR35496; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
PE 1: Evidence at protein level;
KW Allergen; Direct protein sequencing; Disulfide bond; IgE-binding protein;
KW Seed storage protein; Signal; Storage protein.
FT SIGNAL 1..22
FT /evidence="ECO:0000269|PubMed:12372998"
FT CHAIN 23..149
FT /note="2S seed storage albumin protein"
FT /evidence="ECO:0000305|PubMed:12372998"
FT /id="PRO_5010973053"
FT REGION 121..128
FT /note="IgE-binding"
FT /evidence="ECO:0000269|PubMed:20407269"
FT SITE 125
FT /note="Critical for IgE-binding activity"
FT /evidence="ECO:0000269|PubMed:20407269"
FT DISULFID 38..98
FT /evidence="ECO:0000250|UniProtKB:Q647G9"
FT DISULFID 52..87
FT /evidence="ECO:0000250|UniProtKB:Q647G9"
FT DISULFID 88..133
FT /evidence="ECO:0000250|UniProtKB:Q647G9"
FT DISULFID 100..140
FT /evidence="ECO:0000250|UniProtKB:Q647G9"
FT MUTAGEN 1..34
FT /note="Missing: No effect in IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:20054125"
FT MUTAGEN 38
FT /note="C->S: No effect in IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:18520034"
FT MUTAGEN 52
FT /note="C->S: Decreased IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:18520034"
FT MUTAGEN 77
FT /note="C->S: Decreased IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:18520034"
FT MUTAGEN 83
FT /note="C->S: Decreased IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:18520034"
FT MUTAGEN 87
FT /note="C->S: Decreased IgE-binding activity. Susceptible to
FT pepsin digestion by simulated gastric fluid (SGF)."
FT /evidence="ECO:0000269|PubMed:18520034"
FT MUTAGEN 88
FT /note="C->S: Decreased IgE-binding activity. Susceptible to
FT pepsin digestion by simulated gastric fluid (SGF)."
FT /evidence="ECO:0000269|PubMed:18520034"
FT MUTAGEN 98
FT /note="C->S: Decreased IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:18520034"
FT MUTAGEN 100
FT /note="C->S: No effect in IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:18520034"
FT MUTAGEN 120
FT /note="K->A: Decreased IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:20407269"
FT MUTAGEN 121
FT /note="E->A: Decreased IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:20407269"
FT MUTAGEN 122
FT /note="G->A: Decreased IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:20407269"
FT MUTAGEN 123
FT /note="V->A: Decreased IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:20407269"
FT MUTAGEN 124
FT /note="R->A: Decreased IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:20407269"
FT MUTAGEN 125
FT /note="D->A: Significantly decreased IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:20407269"
FT MUTAGEN 126
FT /note="L->A: Decreased IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:20407269"
FT MUTAGEN 127
FT /note="K->A: Decreased IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:20407269"
FT MUTAGEN 128
FT /note="E->A: Decreased IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:20407269"
FT MUTAGEN 133
FT /note="C->S: Decreased IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:18520034"
FT MUTAGEN 140
FT /note="C->S: No effect in IgE-binding activity."
FT /evidence="ECO:0000269|PubMed:18520034"
SQ SEQUENCE 149 AA; 16941 MW; C2F613F49DDBC10C CRC64;
MKLFIILATA TLLIAATQAT YPRDEGFDLG ETQMSSKCMR QVKMNEPHLK KCNRYIAMDI
LDDKYAEALS RVEGEGCKSE ESCMRGCCVA MKEMDDECVC EWMKMMVENQ KGRIGERLIK
EGVRDLKELP SKCGLSELEC GSRGNRYFV
