CRS1_ARATH
ID CRS1_ARATH Reviewed; 720 AA.
AC Q9LF10; F4KCP9;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 2.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic;
DE AltName: Full=Chloroplastic RNA splicing factor 1;
DE AltName: Full=Protein CHLOROPLAST RNA SPLICING 1;
DE Flags: Precursor;
GN OrderedLocusNames=At5g16180; ORFNames=T21H19.100;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17071648; DOI=10.1104/pp.106.088096;
RA Asakura Y., Barkan A.;
RT "Arabidopsis orthologs of maize chloroplast splicing factors promote
RT splicing of orthologous and species-specific group II introns.";
RL Plant Physiol. 142:1656-1663(2006).
CC -!- FUNCTION: Required for the splicing of group IIA introns in
CC chloroplasts, by regulating the intron folding. Forms splicing
CC particles with RNA. May also be involved in chloroplast protein
CC translation. {ECO:0000269|PubMed:17071648}.
CC -!- SUBUNIT: Homodimer. Interacts with RNA. Part of large ribonucleo-
CC protein complexes that include group IIA introns and CRS1 (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Plants are albinos.
CC {ECO:0000269|PubMed:17071648}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAC01859.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL391148; CAC01859.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED92258.1; -; Genomic_DNA.
DR PIR; T51488; T51488.
DR RefSeq; NP_197122.2; NM_121623.3.
DR AlphaFoldDB; Q9LF10; -.
DR SMR; Q9LF10; -.
DR STRING; 3702.AT5G16180.1; -.
DR PaxDb; Q9LF10; -.
DR PRIDE; Q9LF10; -.
DR ProteomicsDB; 222732; -.
DR EnsemblPlants; AT5G16180.1; AT5G16180.1; AT5G16180.
DR GeneID; 831476; -.
DR Gramene; AT5G16180.1; AT5G16180.1; AT5G16180.
DR KEGG; ath:AT5G16180; -.
DR Araport; AT5G16180; -.
DR TAIR; locus:2181372; AT5G16180.
DR eggNOG; KOG1990; Eukaryota.
DR HOGENOM; CLU_006310_2_0_1; -.
DR InParanoid; Q9LF10; -.
DR OMA; ELAMVKF; -.
DR OrthoDB; 505933at2759; -.
DR PRO; PR:Q9LF10; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LF10; baseline and differential.
DR Genevisible; Q9LF10; AT.
DR GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR GO; GO:0003729; F:mRNA binding; IEA:InterPro.
DR GO; GO:0000373; P:Group II intron splicing; IDA:TAIR.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR Gene3D; 3.30.110.60; -; 3.
DR InterPro; IPR045278; CRS1/CFM2/CFM3.
DR InterPro; IPR001890; RNA-binding_CRM.
DR InterPro; IPR035920; YhbY-like_sf.
DR PANTHER; PTHR31846; PTHR31846; 2.
DR Pfam; PF01985; CRS1_YhbY; 3.
DR SMART; SM01103; CRS1_YhbY; 3.
DR SUPFAM; SSF75471; SSF75471; 3.
DR PROSITE; PS51295; CRM; 3.
PE 3: Inferred from homology;
KW Chloroplast; Coiled coil; mRNA processing; mRNA splicing; Plastid;
KW Reference proteome; Repeat; Ribonucleoprotein; RNA-binding;
KW Transit peptide; Translation regulation.
FT TRANSIT 1..77
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 78..720
FT /note="Chloroplastic group IIA intron splicing facilitator
FT CRS1, chloroplastic"
FT /id="PRO_0000283624"
FT DOMAIN 205..301
FT /note="CRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00626"
FT DOMAIN 359..456
FT /note="CRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00626"
FT DOMAIN 570..670
FT /note="CRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00626"
FT COILED 131..159
FT /evidence="ECO:0000255"
FT COILED 447..477
FT /evidence="ECO:0000255"
FT COILED 517..553
FT /evidence="ECO:0000255"
SQ SEQUENCE 720 AA; 83591 MW; 4F3B4000FD77E3C3 CRC64;
MRNGINILSY PRFLSFCEVF VLLLCNFSDN PKLQTFTQML NSLFLSARAF PSLITNSSSR
RAKSSQFDQF RENRGVSDAA IKVPTAPWMK GPLLLRPDEI LDTKKRNKPR KVEEKTFKAL
NRRESGVRGK KAMKKIVRNV EKLDEDSDSE ETQMDDLSEF EYLGRIEEKV ESKDRFGGKM
PWEREEERFI LRRMKKESVP TTAELILDEG LLNRLRREAS KMRKWVNVRK AGVTELVVNK
IKSMWKLNEL AMVRFDVPLC RNMERAQEII EMKTGGLVVL SKKEFLVVYR GGPSYSSEGQ
DEISSSLYER EADRLLDGLG PRYMDWWMRR PFPVDADLLP EVVNGYMTPS RRCPPNTRAK
LTDEELTYLR NIAQPLPFHF VLGRNYGLQG LASAIVKLWE KCIIAKIAIK WGALNTNNEE
MADELRYLTG GVLILRNKYL IVLYRGKDFL SDEVADLVED RERLLSRYQH FEETKRESDI
ELLEVVTNGK QLKETNKSGT LLEFQELQRK FGEMDPRNLE TEAEKARLEK ELKSQEHKLS
ILKSKIEKSN MELFKLNSLW KPSEGDDDIE ILTNEERECL RRIGLKMNSS LVLGRRGVFF
GVMEGLHQHW KHREVAKVIT MQKLFSRVVY TAKALETESN GVLISIEKLK EGHAILIYRG
KNYKRPSSKL MAQNLLTKRK ALQRSVVMQR LGSLKFFAYQ RERAIEDLKV SLVNLQDSAF
