CRS1_ORYSJ
ID CRS1_ORYSJ Reviewed; 725 AA.
AC Q6YYA3; A0A0P0XFH0;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic;
DE AltName: Full=Chloroplastic RNA splicing factor 1;
DE AltName: Full=Protein CHLOROPLAST RNA SPLICING 1;
DE Flags: Precursor;
GN OrderedLocusNames=Os08g0360100, LOC_Os08g27150; ORFNames=OJ1014_E02.14;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Required for the splicing of group IIA introns in
CC chloroplasts, by regulating the intron folding. Forms splicing
CC particles with RNA. Also involved in chloroplast protein translation
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. Interacts with RNA. Part of large ribonucleo-
CC protein complexes that include group IIA introns and CRS1 (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000250}.
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DR EMBL; AP005628; BAD03815.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF23549.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT05104.1; -; Genomic_DNA.
DR EMBL; AK066365; -; NOT_ANNOTATED_CDS; mRNA.
DR AlphaFoldDB; Q6YYA3; -.
DR SMR; Q6YYA3; -.
DR STRING; 4530.OS08T0360100-01; -.
DR PaxDb; Q6YYA3; -.
DR PRIDE; Q6YYA3; -.
DR EnsemblPlants; Os08t0360100-01; Os08t0360100-01; Os08g0360100.
DR Gramene; Os08t0360100-01; Os08t0360100-01; Os08g0360100.
DR eggNOG; KOG1990; Eukaryota.
DR HOGENOM; CLU_006310_2_1_1; -.
DR InParanoid; Q6YYA3; -.
DR OMA; ELAMVKF; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR Genevisible; Q6YYA3; OS.
DR GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR GO; GO:0003729; F:mRNA binding; IEA:InterPro.
DR GO; GO:0000373; P:Group II intron splicing; IEA:EnsemblPlants.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR Gene3D; 3.30.110.60; -; 3.
DR InterPro; IPR045278; CRS1/CFM2/CFM3.
DR InterPro; IPR001890; RNA-binding_CRM.
DR InterPro; IPR035920; YhbY-like_sf.
DR PANTHER; PTHR31846; PTHR31846; 1.
DR Pfam; PF01985; CRS1_YhbY; 3.
DR SMART; SM01103; CRS1_YhbY; 3.
DR SUPFAM; SSF75471; SSF75471; 3.
DR PROSITE; PS51295; CRM; 3.
PE 2: Evidence at transcript level;
KW Chloroplast; Coiled coil; mRNA processing; mRNA splicing; Plastid;
KW Reference proteome; Repeat; Ribonucleoprotein; RNA-binding;
KW Transit peptide; Translation regulation.
FT TRANSIT 1..71
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 72..725
FT /note="Chloroplastic group IIA intron splicing facilitator
FT CRS1, chloroplastic"
FT /id="PRO_0000283626"
FT DOMAIN 153..250
FT /note="CRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00626"
FT DOMAIN 352..449
FT /note="CRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00626"
FT DOMAIN 564..664
FT /note="CRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00626"
FT REGION 1..121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 259..295
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 451..473
FT /evidence="ECO:0000255"
FT COILED 508..545
FT /evidence="ECO:0000255"
FT COILED 691..721
FT /evidence="ECO:0000255"
FT COMPBIAS 1..44
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 77..98
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 107..121
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 259..278
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 279..295
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 279
FT /note="E -> V (in Ref. 4; AK066365)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 725 AA; 82861 MW; DF1045556F4AA6EC CRC64;
MAPPPLHLFS PHVPSTSVSP PDPATEAPPA PKQHRGPRPA PRNPGSAKPL TAGVPGGRTR
RAVLGIIRKV RSLELSDPRT PSPNGGGGSS SSSTATARVP FHLPIHPPPP EREEEEEKKG
IRRAVPWAAA RDEETKVVLR REKKTRVPTR AETELEAGEL ERLRRAARGK ERWARAKKAG
ITDEVVEEVR GQWAKGQELA GVRIVEPLRR CMDRAREILE IKTGGLVVWT RGGIHFVYRG
SSYLENAKRH RDFVNYNEEL SPVTSNNPTS QGKYWSKDET LTNDNDEADD KDDKPIKGTL
YEREVNRLLD SLGPRFIDWW WNTPLPVDAD LLPEVVPDFK TPFRQCPPGV RPALADEELT
YLRKHARPLP THFVLGRNTK LQGLAAAILK LWEKSLIAKV AVKVGIQNTN HEQMARNLKR
LTGGTVILRN KDYIIIYRGK DFLPGGVAES VIERESQVHD QQAKEEEARL KMADSLQMIV
GLSSERSYVG TFREYQDFHD SHARRTTENN FRIQLEAKKH RLEKELKDQE WRLSMLTKKI
ERSNQVLAKL HSSWSPSKKD GDRELLTEEE RRIFRKIGLK MDEHVLLGRR GVFEGVIEEI
HQHWKHKEVV KVITKQNQAS QITYTSMMLE VETGGTLIAI ERFTTSHAII LYRGKNYRRP
TKSAPSNLLT KREALQRSIE VQRRGSMKYF AQERKKSIDE LKRELRNVTW EIRKLNHDTE
QSWTA
