ID   CRS2A_ARATH             Reviewed;         246 AA.
AC   Q9FKN4; Q8GX67;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Chloroplastic group IIB intron splicing facilitator CRS2-A, chloroplastic;
DE   AltName: Full=CRS2-like protein A;
DE   AltName: Full=Chloroplastic RNA splicing factor 2-A;
DE   Flags: Precursor;
GN   Name=CRS2A; OrderedLocusNames=At5g38290; ORFNames=MSI17.10;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA   Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT   features of the regions of 1,381,565 bp covered by twenty one physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:131-145(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11910074; DOI=10.1126/science.1071006;
RA   Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA   Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA   Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA   Shinagawa A., Shinozaki K.;
RT   "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL   Science 296:141-145(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for the splicing of group IIB introns in
CC       chloroplasts. {ECO:0000250}.
CC   -!- SUBUNIT: Part of large ribonucleo-protein complexes that include group
CC       IIB introns and either CAF1 or CAF2. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q9FKN4-1; Sequence=Displayed;
CC   -!- SIMILARITY: Belongs to the PTH family. CRS2 subfamily. {ECO:0000305}.
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DR   EMBL; AB011481; BAB09443.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED94291.1; -; Genomic_DNA.
DR   EMBL; AK118408; BAC43017.1; -; mRNA.
DR   EMBL; BT025030; ABE02405.1; -; mRNA.
DR   RefSeq; NP_198645.1; NM_123190.3. [Q9FKN4-1]
DR   AlphaFoldDB; Q9FKN4; -.
DR   SMR; Q9FKN4; -.
DR   STRING; 3702.AT5G38290.2; -.
DR   PaxDb; Q9FKN4; -.
DR   PRIDE; Q9FKN4; -.
DR   ProteomicsDB; 224508; -. [Q9FKN4-1]
DR   EnsemblPlants; AT5G38290.1; AT5G38290.1; AT5G38290. [Q9FKN4-1]
DR   GeneID; 833811; -.
DR   Gramene; AT5G38290.1; AT5G38290.1; AT5G38290. [Q9FKN4-1]
DR   KEGG; ath:AT5G38290; -.
DR   Araport; AT5G38290; -.
DR   eggNOG; KOG2255; Eukaryota.
DR   HOGENOM; CLU_062456_5_0_1; -.
DR   InParanoid; Q9FKN4; -.
DR   PhylomeDB; Q9FKN4; -.
DR   PRO; PR:Q9FKN4; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FKN4; baseline and differential.
DR   Genevisible; Q9FKN4; AT.
DR   GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR   GO; GO:0004045; F:aminoacyl-tRNA hydrolase activity; IBA:GO_Central.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1470; -; 1.
DR   HAMAP; MF_00083; Pept_tRNA_hydro_bact; 1.
DR   InterPro; IPR001328; Pept_tRNA_hydro.
DR   InterPro; IPR018171; Pept_tRNA_hydro_CS.
DR   InterPro; IPR036416; Pept_tRNA_hydro_sf.
DR   PANTHER; PTHR17224; PTHR17224; 1.
DR   Pfam; PF01195; Pept_tRNA_hydro; 1.
DR   SUPFAM; SSF53178; SSF53178; 1.
DR   TIGRFAMs; TIGR00447; pth; 1.
DR   PROSITE; PS01195; PEPT_TRNA_HYDROL_1; 1.
DR   PROSITE; PS01196; PEPT_TRNA_HYDROL_2; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Chloroplast; mRNA processing; mRNA splicing; Plastid;
KW   Reference proteome; Ribonucleoprotein; Transit peptide.
FT   TRANSIT         1..34
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           35..246
FT                   /note="Chloroplastic group IIB intron splicing facilitator
FT                   CRS2-A, chloroplastic"
FT                   /id="PRO_0000280530"
FT   CONFLICT        143
FT                   /note="M -> L (in Ref. 3; BAC43017)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   246 AA;  27259 MW;  BECEE7A02B679B40 CRC64;
     MFCASSSPIT SPLYPKAYKF SQTKSNSKRF SSLRASLPVS DNKLLKFEYT PWLIVGLGNP
     GLKYYGTRHN IGFEMIDHIA RATDISMNTI QSKALVGIGS VGEVPILLVK PQGYMNFSGE
     SVGPLAAYYQ IPLRHILMIY DDMGLSNGVL RLQPKGGHSQ HNGLKNVTEH LNGCRGYPRL
     SIGIGNPPGN MDMKAFLLQK FSPLERKQMD EGLEQGVEGV KTLVEEGFSD SISRFNLGQK
     YKFHKV