CRS2B_ARATH
ID CRS2B_ARATH Reviewed; 240 AA.
AC Q9LF14;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic;
DE AltName: Full=CRS2-like protein B;
DE AltName: Full=Chloroplastic RNA splicing factor 2-B;
DE Flags: Precursor;
GN Name=CRS2B; OrderedLocusNames=At5g16140; ORFNames=T21H19.60;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Required for the splicing of group IIB introns in
CC chloroplasts. {ECO:0000250}.
CC -!- SUBUNIT: Part of large ribonucleo-protein complexes that include group
CC IIB introns and either CAF1 or CAF2. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PTH family. CRS2 subfamily. {ECO:0000305}.
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DR EMBL; AL391148; CAC01855.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92251.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92252.1; -; Genomic_DNA.
DR EMBL; BT010650; AAR07514.1; -; mRNA.
DR EMBL; AK175292; BAD43055.1; -; mRNA.
DR PIR; T51484; T51484.
DR RefSeq; NP_001078593.1; NM_001085124.3.
DR RefSeq; NP_197118.1; NM_121619.4.
DR AlphaFoldDB; Q9LF14; -.
DR SMR; Q9LF14; -.
DR STRING; 3702.AT5G16140.2; -.
DR iPTMnet; Q9LF14; -.
DR PaxDb; Q9LF14; -.
DR PRIDE; Q9LF14; -.
DR ProteomicsDB; 224411; -.
DR EnsemblPlants; AT5G16140.1; AT5G16140.1; AT5G16140.
DR EnsemblPlants; AT5G16140.2; AT5G16140.2; AT5G16140.
DR GeneID; 831471; -.
DR Gramene; AT5G16140.1; AT5G16140.1; AT5G16140.
DR Gramene; AT5G16140.2; AT5G16140.2; AT5G16140.
DR KEGG; ath:AT5G16140; -.
DR Araport; AT5G16140; -.
DR TAIR; locus:2181412; AT5G16140.
DR eggNOG; KOG2255; Eukaryota.
DR HOGENOM; CLU_062456_5_0_1; -.
DR InParanoid; Q9LF14; -.
DR OMA; HVLSKFH; -.
DR OrthoDB; 1514615at2759; -.
DR PhylomeDB; Q9LF14; -.
DR PRO; PR:Q9LF14; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LF14; baseline and differential.
DR Genevisible; Q9LF14; AT.
DR GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR GO; GO:0004045; F:aminoacyl-tRNA hydrolase activity; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1470; -; 1.
DR HAMAP; MF_00083; Pept_tRNA_hydro_bact; 1.
DR InterPro; IPR001328; Pept_tRNA_hydro.
DR InterPro; IPR018171; Pept_tRNA_hydro_CS.
DR InterPro; IPR036416; Pept_tRNA_hydro_sf.
DR PANTHER; PTHR17224; PTHR17224; 1.
DR Pfam; PF01195; Pept_tRNA_hydro; 1.
DR SUPFAM; SSF53178; SSF53178; 1.
DR TIGRFAMs; TIGR00447; pth; 1.
DR PROSITE; PS01195; PEPT_TRNA_HYDROL_1; 1.
DR PROSITE; PS01196; PEPT_TRNA_HYDROL_2; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; mRNA processing; mRNA splicing; Plastid; Reference proteome;
KW Ribonucleoprotein; Transit peptide.
FT TRANSIT 1..?
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN ?..240
FT /note="Chloroplastic group IIB intron splicing facilitator
FT CRS2-B, chloroplastic"
FT /id="PRO_0000280531"
SQ SEQUENCE 240 AA; 26935 MW; 4A8C368E1A55DDA7 CRC64;
MLCPTYTPSV YHFQTQKPVF RKQRFRVCSS TSGGEDDRFK VEYTPWLIVG LGNPGNKYHG
TRHNVGFEMI DVLARKEGVL MNTIQSKALI GIGAIEDVPI LLAKPQTYMN FSGESVGSLA
SHYRVPLRHI LMIYDEMALP NGVLRLQPKG GQGYHNGVKS VMGHLDGRRN FPRLSIGIGK
PPGNMDMKAF LLQKFSPLEQ KQIEEALEQG SEAVKTLVLN GFNQGISRFN LVQKYKFHKV
