CRSH1_ORYSJ
ID CRSH1_ORYSJ Reviewed; 583 AA.
AC Q6ATB4; A0A0P0WIB5; Q9AYT4;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=GTP diphosphokinase CRSH1, chloroplastic {ECO:0000305};
DE EC=2.7.6.5 {ECO:0000269|PubMed:17938177};
DE AltName: Full=Calcium-activated RelA/Spot homolog 1 {ECO:0000303|PubMed:17938177};
DE Short=OsCRSH1 {ECO:0000303|PubMed:17938177};
DE AltName: Full=ppGpp synthetase CRSH1 {ECO:0000303|PubMed:17938177};
DE Flags: Precursor;
GN Name=CRSH1 {ECO:0000303|PubMed:17938177};
GN Synonyms=RELA2 {ECO:0000303|Ref.1};
GN OrderedLocusNames=Os05g0161200 {ECO:0000312|EMBL:BAS92404.1},
GN LOC_Os05g06890 {ECO:0000305};
GN ORFNames=OsJ_17215 {ECO:0000312|EMBL:EEE62424.1},
GN OSJNBa0034O12.15 {ECO:0000312|EMBL:AAT93920.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare;
RA Tozawa Y., Satsu H., Ito Y., Ochi K.;
RT "Rice cDNA encoding plastid ribosomal protein L11.";
RL Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [8]
RP FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, SUBCELLULAR LOCATION,
RP DOMAIN, AND TISSUE SPECIFICITY.
RX PubMed=17938177; DOI=10.1074/jbc.m703820200;
RA Tozawa Y., Nozawa A., Kanno T., Narisawa T., Masuda S., Kasai K.,
RA Nanamiya H.;
RT "Calcium-activated (p)ppGpp synthetase in chloroplasts of land plants.";
RL J. Biol. Chem. 282:35536-35545(2007).
CC -!- FUNCTION: Possesses calcium-dependent ppGpp (guanosine 3'-diphosphate
CC 5'-diphosphate) synthetase activity in vitro and is able to
CC functionally complement E.coli relA mutants. May be involved in a rapid
CC plant ppGpp-mediated response to pathogens and other stresses.
CC {ECO:0000269|PubMed:17938177}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate;
CC Xref=Rhea:RHEA:22088, ChEBI:CHEBI:30616, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:142410, ChEBI:CHEBI:456215; EC=2.7.6.5;
CC Evidence={ECO:0000269|PubMed:17938177};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:22090;
CC Evidence={ECO:0000269|PubMed:17938177};
CC -!- ACTIVITY REGULATION: Activated by calcium.
CC {ECO:0000269|PubMed:17938177}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:17938177}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and shoots.
CC {ECO:0000269|PubMed:17938177}.
CC -!- DOMAIN: The calcium-binding sites of the 2 EF-hand domains are required
CC for enzyme activity. {ECO:0000305|PubMed:17938177}.
CC -!- SIMILARITY: Belongs to the RelA/SpoT family. {ECO:0000305}.
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DR EMBL; AB042936; BAB21485.1; -; mRNA.
DR EMBL; AC137614; AAT93920.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF16638.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS92404.1; -; Genomic_DNA.
DR EMBL; CM000142; EEE62424.1; -; Genomic_DNA.
DR EMBL; AK121808; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015637549.1; XM_015782063.1.
DR AlphaFoldDB; Q6ATB4; -.
DR SMR; Q6ATB4; -.
DR STRING; 4530.OS05T0161200-01; -.
DR PaxDb; Q6ATB4; -.
DR PRIDE; Q6ATB4; -.
DR EnsemblPlants; Os05t0161200-01; Os05t0161200-01; Os05g0161200.
DR GeneID; 4337888; -.
DR Gramene; Os05t0161200-01; Os05t0161200-01; Os05g0161200.
DR KEGG; osa:4337888; -.
DR eggNOG; KOG1157; Eukaryota.
DR HOGENOM; CLU_022292_1_0_1; -.
DR InParanoid; Q6ATB4; -.
DR OMA; ACYRTHE; -.
DR OrthoDB; 505873at2759; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000007752; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR Genevisible; Q6ATB4; OS.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0008728; F:GTP diphosphokinase activity; IDA:UniProtKB.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0015969; P:guanosine tetraphosphate metabolic process; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00051; EFh; 1.
DR CDD; cd05399; NT_Rel-Spo_like; 1.
DR Gene3D; 3.30.460.10; -; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR006674; HD_domain.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR007685; RelA_SpoT.
DR Pfam; PF13499; EF-hand_7; 1.
DR Pfam; PF04607; RelA_SpoT; 1.
DR SMART; SM00054; EFh; 2.
DR SMART; SM00954; RelA_SpoT; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR SUPFAM; SSF81301; SSF81301; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
DR PROSITE; PS51831; HD; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Calcium; Chloroplast; GTP-binding; Kinase; Metal-binding;
KW Nucleotide-binding; Plastid; Reference proteome; Repeat; Stress response;
KW Transferase; Transit peptide.
FT TRANSIT 1..69
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 70..583
FT /note="GTP diphosphokinase CRSH1, chloroplastic"
FT /id="PRO_0000429854"
FT DOMAIN 119..219
FT /note="HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01175"
FT DOMAIN 473..508
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 510..542
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 1..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..38
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 48..67
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 486
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 488
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 490
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 492
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 497
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 520
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 522
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 524
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 526
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 531
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT CONFLICT 223
FT /note="D -> N (in Ref. 1; BAB21485)"
FT /evidence="ECO:0000305"
FT CONFLICT 525
FT /note="G -> S (in Ref. 7; AK121808)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 583 AA; 64143 MW; 31C6AC538513C54E CRC64;
MATAATTSAA AIPTGGGGRR QHPHPRRPGL RPRRLHRLRL PAQAAAAAAA SSPSTSSSSS
SSSTPAEGGG RLVAELVGAF NELTGRMGEG LATSSSSRLL FRALKLALPA LRDGDGGRAL
ARALAIAASL ADLQMDAEVI SAGILREALD AGAISMRDVK SEIGISTAHL LHESLRLKHA
PSKLDVLDDE SASALRKFCL SYYDIRAVIL ELALKLDMMR HLDCLPRYLQ RIKSLEVLKI
YAPLAHAVGA GNLSLELEDL SFRYLFPHSY DHIDQWLRSQ ETENKLLIDS YKEQLLQALK
DDDELSQIVQ DISIQGRYKS RFSTMKKLVK DGRKPEEVND ILALRVILEP RCDGSSLDWG
PRACHRTHEI IQAMWKEVPG RTKNYVTRPK ENGYQSLHVA IDVSEPGKMR PLMEIQIRTK
EMHKFAVGGE ASHSLYKGGL TDPGEAKRLK AIMLAAAELA AMRLRDLPAS DQGDSNCTNR
AFCQLDKNGD GRISIEELTE VMEDLGAGGK DAKELMHLLD ANSDGSLSSD EFEAFQRQIE
LMRSLDDKDD RYRKILKEKL QTIDSAGLIQ VYRKQLGDKL LVS
