CRT10_YEAST
ID CRT10_YEAST Reviewed; 957 AA.
AC Q08226; D6W204; Q6B2N4; Q7LGP7;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Protein CRT10;
DE AltName: Full=Constitutive RNR transcription regulator 10;
GN Name=CRT10; OrderedLocusNames=YOL063C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP FUNCTION, AND INDUCTION.
RX PubMed=16600900; DOI=10.1093/nar/gkl100;
RA Fu Y., Xiao W.;
RT "Identification and characterization of CRT10 as a novel regulator of
RT Saccharomyces cerevisiae ribonucleotide reductase genes.";
RL Nucleic Acids Res. 34:1876-1883(2006).
RN [6]
RP FUNCTION, INTERACTION WITH MMS1, AND IDENTIFICATION IN A COMPLEX WITH
RP RTT101.
RX PubMed=18704118; DOI=10.1038/embor.2008.155;
RA Zaidi I.W., Rabut G., Poveda A., Scheel H., Malmstrom J., Ulrich H.,
RA Hofmann K., Pasero P., Peter M., Luke B.;
RT "Rtt101 and Mms1 in budding yeast form a CUL4(DDB1)-like ubiquitin ligase
RT that promotes replication through damaged DNA.";
RL EMBO Rep. 9:1034-1040(2008).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-704, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Substrate targeting component of a cullin-RING-based E3
CC ubiquitin-protein ligase complex RTT101(MMS1-CRT10). RTT101(MMS1-CRT10)
CC may regulate nucleotide synthesis through transcriptional regulation of
CC RNR genes encoding ribonucleotide reductases.
CC {ECO:0000269|PubMed:16600900, ECO:0000269|PubMed:18704118}.
CC -!- SUBUNIT: Component of a cullin-RING ligase (CRL) composed of 4
CC subunits: the RING protein HRT1, the cullin RTT101, a linker protein
CC MMS1, and the substrate receptor CRT10. Interacts with MMS1.
CC {ECO:0000269|PubMed:18704118}.
CC -!- INDUCTION: By DNA damage and hydroxyurea (HU).
CC {ECO:0000269|PubMed:16600900}.
CC -!- MISCELLANEOUS: Present with 125 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z74804; CAA99072.1; -; Genomic_DNA.
DR EMBL; Z74805; CAA99073.1; -; Genomic_DNA.
DR EMBL; AY692696; AAT92715.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA10720.1; -; Genomic_DNA.
DR PIR; S66755; S66755.
DR RefSeq; NP_014578.1; NM_001183318.1.
DR AlphaFoldDB; Q08226; -.
DR BioGRID; 34338; 59.
DR DIP; DIP-2832N; -.
DR IntAct; Q08226; 10.
DR MINT; Q08226; -.
DR STRING; 4932.YOL063C; -.
DR iPTMnet; Q08226; -.
DR MaxQB; Q08226; -.
DR PaxDb; Q08226; -.
DR PRIDE; Q08226; -.
DR EnsemblFungi; YOL063C_mRNA; YOL063C; YOL063C.
DR GeneID; 854091; -.
DR KEGG; sce:YOL063C; -.
DR SGD; S000005424; CRT10.
DR VEuPathDB; FungiDB:YOL063C; -.
DR eggNOG; ENOG502RAS5; Eukaryota.
DR HOGENOM; CLU_013329_0_0_1; -.
DR InParanoid; Q08226; -.
DR OMA; YSFRQEY; -.
DR BioCyc; YEAST:G3O-33471-MON; -.
DR PRO; PR:Q08226; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q08226; protein.
DR GO; GO:0070651; P:nonfunctional rRNA decay; IMP:SGD.
DR InterPro; IPR014839; Crt10.
DR Pfam; PF08728; CRT10; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..957
FT /note="Protein CRT10"
FT /id="PRO_0000235920"
FT REGION 695..719
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 704
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT CONFLICT 604
FT /note="P -> S (in Ref. 3; AAT92715)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 957 AA; 109715 MW; EBBB9627C335EFD3 CRC64;
MPPQIPNEND DLFTRWLKSR AIIQRAVSTR ECFDSEVFLA SGGWNITNEI ITLKKYYQLK
WPNSSCNSFH PKTVEFIKER LHNLEEHDSS WKIPNPAYSF KKAFLEDTKS AFSNLEPVWG
PSRLLNPAEL LLPQDEKLLV QEIPLEFAPF QYTNRFAYGG LQFKNNLFVT YGSYSFLAAG
QCVEVHNFDI LLNVSSLEIC HALLPVIIPD DGDVRNFRNS SYVKFKDTQF NSIPELCSIN
FMKICNFMHQ DFLLACGDNG IVYIWEINKV IKIFNKFTSD ILGGKDNSRE RYINVDPYMV
LRVEESCWSV DVIDINGIIY IAVGHNKPGV TVFAFDKDVK KERRYIRPLD LPSSHNVPCV
NFVPNSKDSV GYITLSYCSI FGNVVTVKLK EHDCTILTSF LDTQFFGDDL WTITPLTKKD
FAKVDNFELL NLNYQDGFKE SMLYSICRDD FLLGYYCDNA YLSGNFGIGT LLNQFQVPVT
DLRLTSSAGI PDEVIPLRFT SFDRNYTTTG SIKYEYSRED FALILHAGDL DDMNDAVTKN
TSCEQHLHQW TFWEDSGYKH YRATERGFSK YKDIINTFPQ LITPSGRNKT SQYQNTSGRK
ICEPSTYKLT DLENDIEDIS REFNRSIRNL KMDKQRQLRT SKEFKSLSSV NHIPNIESGN
FLWYNTDAAA DWRTLFGKDL NTVLKDPEIC SLQLNSTEED DVNSDPENEE SGSSLTSFQR
RYRDTEQRAH LKSESQKSWG FHNYVRNVKR LLESAVPGSE DSPLGYQLSE MHDEFFFLTT
AHRLVLMKAN PLIIISATHH EIFPLDGVVT CASKSLLQAL NRINFVCHIK ELNCIAVASQ
LGLISLLRLT EYRGIYSFRQ EYILGWEVQD PVNPSPECRC NRNLFDAPMY GADGESSDTY
CGVCDVYFPM GDICGLDYTY ASDSEELKRK GYATLYVASR GSLRAFKITT EHGTTQQ
