CRTA_RHOCB
ID CRTA_RHOCB Reviewed; 241 AA.
AC P17055; D5AP71;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 2.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Spheroidene monooxygenase;
DE EC=1.14.15.9 {ECO:0000269|PubMed:19136077, ECO:0000269|PubMed:20851979};
GN Name=crtA; OrderedLocusNames=RCAP_rcc00678;
OS Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Rhodobacter.
OX NCBI_TaxID=272942;
RN [1]
RP PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX PubMed=2747617; DOI=10.1007/bf00334364;
RA Armstrong G.A., Alberti M., Leach F., Hearst J.E.;
RT "Nucleotide sequence, organization, and nature of the protein products of
RT the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus.";
RL Mol. Gen. Genet. 216:254-268(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RA Burke D.H., Alberti M., Armstrong G.A., Hearst J.E.;
RL Submitted (NOV-1991) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX PubMed=20418398; DOI=10.1128/jb.00366-10;
RA Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V.,
RA Haselkorn R.;
RT "Complete genome sequence of the photosynthetic purple nonsulfur bacterium
RT Rhodobacter capsulatus SB 1003.";
RL J. Bacteriol. 192:3545-3546(2010).
RN [4]
RP FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=19136077; DOI=10.1016/j.bbalip.2008.12.006;
RA Gerjets T., Steiger S., Sandmann G.;
RT "Catalytic properties of the expressed acyclic carotenoid 2-ketolases from
RT Rhodobacter capsulatus and Rubrivivax gelatinosus.";
RL Biochim. Biophys. Acta 1791:125-131(2009).
RN [5]
RP FUNCTION, CATALYTIC ACTIVITY, COFACTOR, AND SUBSTRATE SPECIFICITY.
RX PubMed=20851979; DOI=10.1128/aem.01606-10;
RA Lee P.C., Holtzapple E., Schmidt-Dannert C.;
RT "Novel activity of Rhodobacter sphaeroides spheroidene monooxygenase CrtA
RT expressed in Escherichia coli.";
RL Appl. Environ. Microbiol. 76:7328-7331(2010).
CC -!- FUNCTION: Catalyzes the asymmetrical introduction of one keto group at
CC the C-2 position of spheroidene and two keto groups at the C-2 and C-2'
CC positions of spirilloxanthin. {ECO:0000269|PubMed:19136077,
CC ECO:0000269|PubMed:20851979}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4 H(+) + 2 O2 + 4 reduced [2Fe-2S]-[ferredoxin] + spheroidene
CC = 3 H2O + 4 oxidized [2Fe-2S]-[ferredoxin] + spheroiden-2-one;
CC Xref=Rhea:RHEA:33027, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:35330,
CC ChEBI:CHEBI:62480; EC=1.14.15.9;
CC Evidence={ECO:0000269|PubMed:19136077, ECO:0000269|PubMed:20851979};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4 H(+) + 2 O2 + 4 reduced [2Fe-2S]-[ferredoxin] +
CC spirilloxanthin = 2-oxospirilloxanthin + 3 H2O + 4 oxidized [2Fe-2S]-
CC [ferredoxin]; Xref=Rhea:RHEA:33039, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:35328,
CC ChEBI:CHEBI:64792; EC=1.14.15.9;
CC Evidence={ECO:0000269|PubMed:19136077, ECO:0000269|PubMed:20851979};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-oxospirilloxanthin + 4 H(+) + 2 O2 + 4 reduced [2Fe-2S]-
CC [ferredoxin] = 2,2'-dioxospirilloxanthin + 3 H2O + 4 oxidized [2Fe-
CC 2S]-[ferredoxin]; Xref=Rhea:RHEA:33035, Rhea:RHEA-COMP:10000,
CC Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738,
CC ChEBI:CHEBI:64792, ChEBI:CHEBI:64793; EC=1.14.15.9;
CC Evidence={ECO:0000269|PubMed:19136077, ECO:0000269|PubMed:20851979};
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000269|PubMed:20851979};
CC Note=Binds 1 heme per subunit. {ECO:0000269|PubMed:20851979};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=19.1 uM for spheroidene {ECO:0000269|PubMed:19136077};
CC KM=10.1 uM for HO-spheroidene {ECO:0000269|PubMed:19136077};
CC KM=13.6 uM for spirilloxanthin {ECO:0000269|PubMed:19136077};
CC Vmax=1.31 umol/h/mg enzyme toward spheroiden-2-one
CC {ECO:0000269|PubMed:19136077};
CC Vmax=1.212 umol/h/mg enzyme toward HO-spheroiden-2-one
CC {ECO:0000269|PubMed:19136077};
CC Vmax=0.357 umol/h/mg enzyme toward 2,2'-dioxospirilloxanthin
CC {ECO:0000269|PubMed:19136077};
CC -!- PATHWAY: Carotenoid biosynthesis; spheroidene biosynthesis.
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DR EMBL; X52291; CAA36532.1; -; Genomic_DNA.
DR EMBL; Z11165; CAA77539.1; -; Genomic_DNA.
DR EMBL; CP001312; ADE84443.1; -; Genomic_DNA.
DR PIR; S04401; S04401.
DR RefSeq; WP_013066422.1; NC_014034.1.
DR AlphaFoldDB; P17055; -.
DR STRING; 272942.RCAP_rcc00678; -.
DR EnsemblBacteria; ADE84443; ADE84443; RCAP_rcc00678.
DR GeneID; 31489624; -.
DR KEGG; rcp:RCAP_rcc00678; -.
DR eggNOG; ENOG5030G1W; Bacteria.
DR HOGENOM; CLU_064051_0_0_5; -.
DR OMA; VTFSIWP; -.
DR OrthoDB; 1663924at2; -.
DR BioCyc; MetaCyc:MON-14936; -.
DR UniPathway; UPA00683; -.
DR Proteomes; UP000002361; Chromosome.
DR GO; GO:0043823; F:spheroidene monooxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Carotenoid biosynthesis; Chlorophyll biosynthesis; Oxidoreductase;
KW Photosynthesis; Reference proteome.
FT CHAIN 1..241
FT /note="Spheroidene monooxygenase"
FT /id="PRO_0000079363"
SQ SEQUENCE 241 AA; 27004 MW; 45531D8636D4D8CB CRC64;
MPVASLSLFR FDGTSSLPWV ISQMILSRRP LNDEPRVKFY KLCGSGTGEG FTPKPNWRVW
AIMAAFDTEA DARDVTANHP VWKRWRAHAA ETLVLHLQPL SARGTWGGVN PFLPEQVAEP
SPDEPVVALT RAAIKPHKAN AFWSRVPKIS EKVGEDQNLM FKIGIGEIPL FHQVTFSIWP
DVAKMNAFAR GDTPHGKAIR AAREEGWFTE ELYARFRLLG TEGSWMGKDP LASKVLERET
A
