CRTB_CERS4
ID CRTB_CERS4 Reviewed; 355 AA.
AC P54905; Q3J191; Q9RFC9;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 2.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=Phytoene synthase;
DE Short=PSase;
DE EC=2.5.1.-;
GN Name=crtB; OrderedLocusNames=RHOS4_18750; ORFNames=RSP_0270;
OS Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG
OS 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) (Rhodobacter
OS sphaeroides).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Cereibacter.
OX NCBI_TaxID=272943;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8021167; DOI=10.1128/jb.176.13.3859-3869.1994;
RA Lang H.P., Cogdell R.J., Gardiner A.T., Hunter C.N.;
RT "Early steps in carotenoid biosynthesis: sequences and transcriptional
RT analysis of the crtI and crtB genes of Rhodobacter sphaeroides and
RT overexpression and reactivation of crtI in Escherichia coli and R.
RT sphaeroides.";
RL J. Bacteriol. 176:3859-3869(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7721699; DOI=10.1128/jb.177.8.2064-2073.1995;
RA Lang H.P., Cogdell R.J., Takaichi S., Hunter C.N.;
RT "Complete DNA sequence, specific Tn5 insertion map, and gene assignment of
RT the carotenoid biosynthesis pathway of Rhodobacter sphaeroides.";
RL J. Bacteriol. 177:2064-2073(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10648776; DOI=10.1093/nar/28.4.862;
RA Choudhary M., Kaplan S.;
RT "DNA sequence analysis of the photosynthesis region of Rhodobacter
RT sphaeroides 2.4.1.";
RL Nucleic Acids Res. 28:862-867(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203
RC / NCIMB 8253 / ATH 2.4.1.;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C.,
RA Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.;
RT "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1.";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the biosynthesis of carotenoids. Catalyzes the
CC condensation of two molecules of geranylgeranyl diphosphate (GGPP) to
CC give prephytoene diphosphate (PPPP) and the subsequent rearrangement of
CC the cyclopropylcarbinyl intermediate to yield phytoene (By similarity).
CC {ECO:0000250}.
CC -!- COFACTOR:
CC Name=ATP; Xref=ChEBI:CHEBI:30616; Evidence={ECO:0000250};
CC Note=ATP is required for the transferase activity but it does not seem
CC to be hydrolyzed during the reaction. {ECO:0000250};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis.
CC -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC {ECO:0000305}.
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DR EMBL; S71770; AAB31139.1; -; Genomic_DNA.
DR EMBL; AJ010302; CAB38740.1; -; Genomic_DNA.
DR EMBL; AF195122; AAF24290.1; -; Genomic_DNA.
DR EMBL; CP000143; ABA79443.1; -; Genomic_DNA.
DR PIR; S49621; S49621.
DR PIR; T50746; T50746.
DR RefSeq; WP_011338116.1; NZ_CP030271.1.
DR RefSeq; YP_353344.1; NC_007493.2.
DR AlphaFoldDB; P54905; -.
DR SMR; P54905; -.
DR STRING; 272943.RSP_0270; -.
DR EnsemblBacteria; ABA79443; ABA79443; RSP_0270.
DR KEGG; rsp:RSP_0270; -.
DR PATRIC; fig|272943.9.peg.2213; -.
DR eggNOG; COG1562; Bacteria.
DR OMA; KLYCYRV; -.
DR PhylomeDB; P54905; -.
DR UniPathway; UPA00799; -.
DR Proteomes; UP000002703; Chromosome 1.
DR GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016117; P:carotenoid biosynthetic process; ISS:UniProtKB.
DR CDD; cd00683; Trans_IPPS_HH; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR002060; Squ/phyt_synthse.
DR InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR InterPro; IPR044843; Trans_IPPS_bact-type.
DR InterPro; IPR033904; Trans_IPPS_HH.
DR Pfam; PF00494; SQS_PSY; 1.
DR SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR SFLD; SFLDG01018; Squalene/Phytoene_Synthase_Lik; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
DR PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE 3: Inferred from homology;
KW Carotenoid biosynthesis; Magnesium; Manganese; Metal-binding;
KW Reference proteome; Transferase.
FT CHAIN 1..355
FT /note="Phytoene synthase"
FT /id="PRO_0000067434"
FT CONFLICT 219
FT /note="L -> Q (in Ref. 3; AAF24290)"
FT /evidence="ECO:0000305"
FT CONFLICT 286
FT /note="A -> S (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 324
FT /note="G -> R (in Ref. 3; AAF24290)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 355 AA; 38987 MW; F5437EBDBF661825 CRC64;
MIASADLDAC REMIRTGSYS FHAASRLLPE RVRAPSLALY AFCRVADDAV DEAVNDGQRE
EDAEVKRRAV LSLRDRLDLV YGGRPRNAPA DRAFAAVVEE FEMPRALPEA LLEGLAWDAV
GRSYDSFSGV LDYSARVAAA VGAMMCVLMR VRDPDVLARA CDLGLAMQLT NIARDVGTDA
RSGRIYLPRD WMEEEGLPVE EFLARPVVDD RIRAVTHRLL RAADRLYLRS EAGVCGLPLA
CRPGIYAARH IYAGIGDEIA RNGYDSVTRR AFTTRRQKLV WLGLSATRAA LSPFGPGCAT
LHAAPEPEVA FLVNAAARAR PQRGRSEALI SVLAQLEAQD RQISRQRLGN RANPI
