ID   CRTB_ENTAG              Reviewed;         295 AA.
AC   D5KXJ0;
DT   24-JUL-2013, integrated into UniProtKB/Swiss-Prot.
DT   15-JUN-2010, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=15-cis-phytoene synthase {ECO:0000305};
DE            EC=2.5.1.32 {ECO:0000269|PubMed:12641468};
DE   AltName: Full=Phytoene synthase;
DE            Short=PSase;
GN   Name=crtB;
OS   Enterobacter agglomerans (Erwinia herbicola) (Pantoea agglomerans).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Pantoea; Pantoea agglomerans group.
OX   NCBI_TaxID=549;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=206;
RA   Galinousky D.V.;
RT   "Sequencing of the bacterial phytoene synthase gene and comparing with psy-
RT   gene of plants.";
RL   Vestsi Natsyianalnai Akademii Navuk Belarusi Ser. Biyalagichnykh Navuk
RL   1:30-34(2010).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, AND
RP   ACTIVITY REGULATION.
RX   PubMed=12641468; DOI=10.1021/bi0206614;
RA   Iwata-Reuyl D., Math S.K., Desai S.B., Poulter C.D.;
RT   "Bacterial phytoene synthase: molecular cloning, expression, and
RT   characterization of Erwinia herbicola phytoene synthase.";
RL   Biochemistry 42:3359-3365(2003).
CC   -!- FUNCTION: Involved in the biosynthesis of carotenoids. Catalyzes
CC       stereoselectively the condensation of two molecules of geranylgeranyl
CC       diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the
CC       subsequent rearrangement of the cyclopropylcarbinyl intermediate to
CC       yield 15-cis-phytoene. {ECO:0000269|PubMed:12641468}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2
CC         diphosphate; Xref=Rhea:RHEA:34475, ChEBI:CHEBI:27787,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58756; EC=2.5.1.32;
CC         Evidence={ECO:0000269|PubMed:12641468};
CC   -!- COFACTOR:
CC       Name=ATP; Xref=ChEBI:CHEBI:30616;
CC         Evidence={ECO:0000269|PubMed:12641468};
CC       Note=ATP is required for the transferase activity but it does not seem
CC       to be hydrolyzed during the reaction. {ECO:0000269|PubMed:12641468};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000269|PubMed:12641468};
CC   -!- ACTIVITY REGULATION: Significant inhibition is seen at GGPP
CC       concentrations above 100 uM. {ECO:0000269|PubMed:12641468}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 8.2. {ECO:0000269|PubMed:12641468};
CC   -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis.
CC   -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC       {ECO:0000305}.
CC   -!- CAUTION: The enzyme produces 15-cis-phytoene. The conversion to all-
CC       trans-phytoene is due to photoisomerisation.
CC       {ECO:0000305|PubMed:12641468}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; GU721093; ADD97675.1; -; Genomic_DNA.
DR   AlphaFoldDB; D5KXJ0; -.
DR   SMR; D5KXJ0; -.
DR   STRING; 549.BW31_02395; -.
DR   KEGG; ag:ADD97675; -.
DR   eggNOG; COG1562; Bacteria.
DR   UniPathway; UPA00799; -.
DR   GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR   GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016765; F:transferase activity, transferring alkyl or aryl (other than methyl) groups; IDA:UniProtKB.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IDA:UniProtKB.
DR   CDD; cd00683; Trans_IPPS_HH; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR002060; Squ/phyt_synthse.
DR   InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR   InterPro; IPR044843; Trans_IPPS_bact-type.
DR   InterPro; IPR033904; Trans_IPPS_HH.
DR   Pfam; PF00494; SQS_PSY; 1.
DR   SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR   SFLD; SFLDG01018; Squalene/Phytoene_Synthase_Lik; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
DR   PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR   PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; Manganese; Metal-binding; Transferase.
FT   CHAIN           1..295
FT                   /note="15-cis-phytoene synthase"
FT                   /id="PRO_0000423168"
SQ   SEQUENCE   295 AA;  32786 MW;  7F366B6FFF83956C CRC64;
     MEVGSKSFAT ASKLFDAKTR RSVLMLYAWC RHCDDVIDDQ VLGFSNDTPS LQSAEQRLAQ
     LEMKTRQPMR IQMHEPAFAA FQEVAMAHDI LPAYAFDHLA GFAMDVHETR YQTLDDTLRY
     CYHVAGVVGL MMAQIMGVRD NATLDRACDL GLAFQLTNIA RDIVEDAEAG RCYLPAAWLA
     EEGLTRENLA DPQNRKALSR VARRLVETAE PYYRSASAGL PGLPLRSAWA IATAQQVYRK
     IGMKVVQAGS QAWEQRQSTS TPEKLALLVA ASGQAVTSRV ARHAPRSADL WQRPV