CRTB_MYCBO
ID CRTB_MYCBO Reviewed; 302 AA.
AC P65861; A0A1R3Y446; Q50728; X2BN51;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=Phytoene synthase;
DE Short=PSase;
DE EC=2.5.1.-;
GN Name=crtB; Synonyms=phyA; OrderedLocusNames=BQ2027_MB3430C;
OS Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=233413;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT "The complete genome sequence of Mycobacterium bovis.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA Robbe-Austerman S., Gordon S.V.;
RT "Updated reference genome sequence and annotation of Mycobacterium bovis
RT AF2122/97.";
RL Genome Announc. 5:E00157-E00157(2017).
CC -!- FUNCTION: Involved in the biosynthesis of carotenoids. Catalyzes the
CC condensation of two molecules of geranylgeranyl diphosphate (GGPP) to
CC give prephytoene diphosphate (PPPP) and the subsequent rearrangement of
CC the cyclopropylcarbinyl intermediate to yield phytoene (Probable).
CC {ECO:0000305}.
CC -!- COFACTOR:
CC Name=ATP; Xref=ChEBI:CHEBI:30616; Evidence={ECO:0000250};
CC Note=ATP is required for the transferase activity but it does not seem
CC to be hydrolyzed during the reaction. {ECO:0000250};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis.
CC -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC {ECO:0000305}.
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DR EMBL; LT708304; SIU02059.1; -; Genomic_DNA.
DR RefSeq; NP_857071.1; NC_002945.3.
DR RefSeq; WP_003417954.1; NC_002945.4.
DR AlphaFoldDB; P65861; -.
DR SMR; P65861; -.
DR EnsemblBacteria; SIU02059; SIU02059; BQ2027_MB3430C.
DR GeneID; 45427393; -.
DR PATRIC; fig|233413.5.peg.3765; -.
DR OMA; VKAPRIM; -.
DR UniPathway; UPA00799; -.
DR Proteomes; UP000001419; Chromosome.
DR GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016117; P:carotenoid biosynthetic process; ISS:UniProtKB.
DR CDD; cd00683; Trans_IPPS_HH; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR017828; SQ_synth_HpnD-like.
DR InterPro; IPR002060; Squ/phyt_synthse.
DR InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR InterPro; IPR044843; Trans_IPPS_bact-type.
DR InterPro; IPR033904; Trans_IPPS_HH.
DR Pfam; PF00494; SQS_PSY; 1.
DR SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
DR TIGRFAMs; TIGR03465; HpnD; 1.
DR PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE 3: Inferred from homology;
KW Carotenoid biosynthesis; Magnesium; Manganese; Metal-binding; Transferase.
FT CHAIN 1..302
FT /note="Phytoene synthase"
FT /id="PRO_0000067432"
SQ SEQUENCE 302 AA; 33142 MW; C214605A7B93AFFD CRC64;
MTEIEQAYRI TESITRTAAR NFYYGIRLLP REKRAALSAV YALGRRIDDV ADGELAPETK
ITELDAIRKS LDNIDDSSDP VLVALADAAR RFPVPIAMFA ELIDGARMEI DWTGCRDFDE
LIVYCRRGAG TIGKLCLSIF GPVSTATSRY AEQLGIALQQ TNILRDVRED FLNGRIYLPR
DELDRLGVRL RLDDTGALDD PDGRLAALLR FSADRAADWY SLGLRLIPHL DRRSAACCAA
MSGIYRRQLA LIRASPAVVY DRRISLSGLK KAQVAAAALA SSVTCGPAHG PLPADLGSHP
SH