ID   CRTB_MYCTU              Reviewed;         302 AA.
AC   P9WHP3; L0TCN1; P65860; Q50728;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Phytoene synthase;
DE            Short=PSase;
DE            EC=2.5.1.-;
GN   Name=crtB; Synonyms=phyA; OrderedLocusNames=Rv3397c; ORFNames=MTCY78.31;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Involved in the biosynthesis of carotenoids. Catalyzes the
CC       condensation of two molecules of geranylgeranyl diphosphate (GGPP) to
CC       give prephytoene diphosphate (PPPP) and the subsequent rearrangement of
CC       the cyclopropylcarbinyl intermediate to yield phytoene (By similarity).
CC       {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=ATP; Xref=ChEBI:CHEBI:30616; Evidence={ECO:0000250};
CC       Note=ATP is required for the transferase activity but it does not seem
CC       to be hydrolyzed during the reaction. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis.
CC   -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC       {ECO:0000305}.
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DR   EMBL; AL123456; CCP46219.1; -; Genomic_DNA.
DR   PIR; B70735; B70735.
DR   RefSeq; NP_217914.1; NC_000962.3.
DR   RefSeq; WP_003417954.1; NZ_NVQJ01000027.1.
DR   AlphaFoldDB; P9WHP3; -.
DR   SMR; P9WHP3; -.
DR   STRING; 83332.Rv3397c; -.
DR   PaxDb; P9WHP3; -.
DR   DNASU; 887911; -.
DR   GeneID; 45427393; -.
DR   GeneID; 887911; -.
DR   KEGG; mtu:Rv3397c; -.
DR   TubercuList; Rv3397c; -.
DR   eggNOG; COG1562; Bacteria.
DR   OMA; VKAPRIM; -.
DR   PhylomeDB; P9WHP3; -.
DR   UniPathway; UPA00799; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR   GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; ISS:UniProtKB.
DR   CDD; cd00683; Trans_IPPS_HH; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR017828; SQ_synth_HpnD-like.
DR   InterPro; IPR002060; Squ/phyt_synthse.
DR   InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR   InterPro; IPR044843; Trans_IPPS_bact-type.
DR   InterPro; IPR033904; Trans_IPPS_HH.
DR   Pfam; PF00494; SQS_PSY; 1.
DR   SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
DR   TIGRFAMs; TIGR03465; HpnD; 1.
DR   PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR   PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; Magnesium; Manganese; Metal-binding;
KW   Reference proteome; Transferase.
FT   CHAIN           1..302
FT                   /note="Phytoene synthase"
FT                   /id="PRO_0000067431"
SQ   SEQUENCE   302 AA;  33142 MW;  C214605A7B93AFFD CRC64;
     MTEIEQAYRI TESITRTAAR NFYYGIRLLP REKRAALSAV YALGRRIDDV ADGELAPETK
     ITELDAIRKS LDNIDDSSDP VLVALADAAR RFPVPIAMFA ELIDGARMEI DWTGCRDFDE
     LIVYCRRGAG TIGKLCLSIF GPVSTATSRY AEQLGIALQQ TNILRDVRED FLNGRIYLPR
     DELDRLGVRL RLDDTGALDD PDGRLAALLR FSADRAADWY SLGLRLIPHL DRRSAACCAA
     MSGIYRRQLA LIRASPAVVY DRRISLSGLK KAQVAAAALA SSVTCGPAHG PLPADLGSHP
     SH