ID   CRTB_PANAN              Reviewed;         309 AA.
AC   P21683;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2003, sequence version 2.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=15-cis-phytoene synthase {ECO:0000305};
DE            Short=PSase;
DE            EC=2.5.1.32 {ECO:0000269|PubMed:9593819};
GN   Name=crtB;
OS   Pantoea ananas (Erwinia uredovora).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Pantoea.
OX   NCBI_TaxID=553;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 19321 / DSM 30080 / NCPPB 800 / NRRL B-14773 / 20D3;
RX   PubMed=2254247; DOI=10.1128/jb.172.12.6704-6712.1990;
RA   Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K.,
RA   Harashima K.;
RT   "Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by
RT   functional analysis of gene products expressed in Escherichia coli.";
RL   J. Bacteriol. 172:6704-6712(1990).
RN   [2]
RP   SEQUENCE REVISION TO N-TERMINUS.
RA   Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K.,
RA   Harashima K.;
RL   Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ACTIVITY
RP   REGULATION, AND COFACTOR.
RX   PubMed=9593819; DOI=10.1016/s0005-2760(98)00017-4;
RA   Neudert U., Martinez-Ferez I.M., Fraser P.D., Sandmann G.;
RT   "Expression of an active phytoene synthase from Erwinia uredovora and
RT   biochemical properties of the enzyme.";
RL   Biochim. Biophys. Acta 1392:51-58(1998).
CC   -!- FUNCTION: Involved in the biosynthesis of carotenoids. Catalyzes the
CC       condensation of two molecules of geranylgeranyl diphosphate (GGPP) to
CC       give prephytoene diphosphate (PPPP) and the subsequent rearrangement of
CC       the cyclopropylcarbinyl intermediate to yield the 15-cis-phytoene
CC       isomer. {ECO:0000269|PubMed:9593819}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2
CC         diphosphate; Xref=Rhea:RHEA:34475, ChEBI:CHEBI:27787,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58756; EC=2.5.1.32;
CC         Evidence={ECO:0000269|PubMed:9593819};
CC   -!- COFACTOR:
CC       Name=ATP; Xref=ChEBI:CHEBI:30616;
CC         Evidence={ECO:0000269|PubMed:9593819};
CC       Note=ATP is required for the transferase activity but it does not seem
CC       to be hydrolyzed during the reaction. {ECO:0000269|PubMed:9593819};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000269|PubMed:9593819};
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000269|PubMed:9593819};
CC   -!- ACTIVITY REGULATION: Inhibited by phosphate ions and squalestatin.
CC       {ECO:0000269|PubMed:9593819}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=41 uM for GGPP (at pH 8 and 37 degrees Celsius)
CC         {ECO:0000269|PubMed:9593819};
CC   -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis.
CC   -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC       {ECO:0000305}.
CC   -!- CAUTION: The enzyme produces 15-cis-phytoene. The conversion to all-
CC       trans-phytoene is due to photoisomerisation.
CC       {ECO:0000250|UniProtKB:D5KXJ0}.
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DR   EMBL; D90087; BAA14128.2; -; Genomic_DNA.
DR   PIR; E37802; E37802.
DR   RefSeq; WP_013027995.1; NZ_WHOW01000007.1.
DR   AlphaFoldDB; P21683; -.
DR   SMR; P21683; -.
DR   GeneID; 57270484; -.
DR   SABIO-RK; P21683; -.
DR   UniPathway; UPA00799; -.
DR   GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR   GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IDA:UniProtKB.
DR   CDD; cd00683; Trans_IPPS_HH; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR002060; Squ/phyt_synthse.
DR   InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR   InterPro; IPR044843; Trans_IPPS_bact-type.
DR   InterPro; IPR033904; Trans_IPPS_HH.
DR   Pfam; PF00494; SQS_PSY; 1.
DR   SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR   SFLD; SFLDG01018; Squalene/Phytoene_Synthase_Lik; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
DR   PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR   PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; Magnesium; Manganese; Metal-binding; Transferase.
FT   CHAIN           1..309
FT                   /note="15-cis-phytoene synthase"
FT                   /id="PRO_0000067429"
FT   REGION          290..309
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   309 AA;  34472 MW;  9AA381A7376BBFC9 CRC64;
     MNNPSLLNHA VETMAVGSKS FATASKLFDA KTRRSVLMLY AWCRHCDDVI DDQTLGFQAR
     QPALQTPEQR LMQLEMKTRQ AYAGSQMHEP AFAAFQEVAM AHDIAPAYAF DHLEGFAMDV
     REAQYSQLDD TLRYCYHVAG VVGLMMAQIM GVRDNATLDR ACDLGLAFQL TNIARDIVDD
     AHAGRCYLPA SWLEHEGLNK ENYAAPENRQ ALSRIARRLV QEAEPYYLSA TAGLAGLPLR
     SAWAIATAKQ VYRKIGVKVE QAGQQAWDQR QSTTTPEKLT LLLAASGQAL TSRMRAHPPR
     PAHLWQRPL