ID   CRTB_PSEVU              Reviewed;         309 AA.
AC   P22872;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Phytoene synthase;
DE            Short=PSase;
DE            EC=2.5.1.-;
GN   Name=crtB;
OS   Pseudescherichia vulneris (Escherichia vulneris).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Pseudescherichia.
OX   NCBI_TaxID=566;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 39368 / Eho10;
RX   PubMed=2263648; DOI=10.1073/pnas.87.24.9975;
RA   Armstrong G.A., Alberti M., Hearst J.E.;
RT   "Conserved enzymes mediate the early reactions of carotenoid biosynthesis
RT   in nonphotosynthetic and photosynthetic prokaryotes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:9975-9979(1990).
RN   [2]
RP   FUNCTION.
RX   PubMed=1495965; DOI=10.1073/pnas.89.15.6761;
RA   Math S.K., Hearst J.E., Poulter C.D.;
RT   "The crtE gene in Erwinia herbicola encodes geranylgeranyl diphosphate
RT   synthase.";
RL   Proc. Natl. Acad. Sci. U.S.A. 89:6761-6764(1992).
CC   -!- FUNCTION: Involved in the biosynthesis of carotenoids. Catalyzes the
CC       condensation of two molecules of geranylgeranyl diphosphate (GGPP) to
CC       give prephytoene diphosphate (PPPP) and the subsequent rearrangement of
CC       the cyclopropylcarbinyl intermediate to yield phytoene (Probable).
CC       {ECO:0000305|PubMed:1495965}.
CC   -!- COFACTOR:
CC       Name=ATP; Xref=ChEBI:CHEBI:30616; Evidence={ECO:0000250};
CC       Note=ATP is required for the transferase activity but it does not seem
CC       to be hydrolyzed during the reaction. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis.
CC   -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC       {ECO:0000305}.
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DR   EMBL; M38423; AAA24821.1; -; Genomic_DNA.
DR   EMBL; M87280; AAA64982.1; -; Genomic_DNA.
DR   PIR; B39273; B39273.
DR   AlphaFoldDB; P22872; -.
DR   SMR; P22872; -.
DR   UniPathway; UPA00799; -.
DR   GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR   GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; ISS:UniProtKB.
DR   CDD; cd00683; Trans_IPPS_HH; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR002060; Squ/phyt_synthse.
DR   InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR   InterPro; IPR044843; Trans_IPPS_bact-type.
DR   InterPro; IPR033904; Trans_IPPS_HH.
DR   Pfam; PF00494; SQS_PSY; 1.
DR   SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR   SFLD; SFLDG01018; Squalene/Phytoene_Synthase_Lik; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
DR   PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR   PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE   3: Inferred from homology;
KW   Carotenoid biosynthesis; Magnesium; Manganese; Metal-binding; Transferase.
FT   CHAIN           1..309
FT                   /note="Phytoene synthase"
FT                   /id="PRO_0000067428"
SQ   SEQUENCE   309 AA;  34123 MW;  B64235C9E0C06F38 CRC64;
     MSQPPLLDHA TQTMANGSKS FATAAKLFDP ATRRSVLMLY TWCRHCDDVI DDQTHGFASE
     AAAEEEATQR LARLRTLTLA AFEGAEMQDP AFAAFQEVAL THGITPRMAL DHLDGFAMDV
     AQTRYVTFED TLRYCYHVAG VVGLMMARVM GVRDERVLDR ACDLGLAFQL TNIARDIIDD
     AAIDRCYLPA EWLQDAGLTP ENYAARENRA ALARVAERLI DAAEPYYISS QAGLHDLPPR
     CAWAIATARS VYREIGIKVK AAGGSAWDRR QHTSKGEKIA MLMAAPGQVI RAKTTRVTPR
     PAGLWQRPV