ID   CRTB_RHOCB              Reviewed;         339 AA.
AC   P17056; D5AP73;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Phytoene synthase;
DE            Short=PSase;
DE            EC=2.5.1.-;
GN   Name=crtB; OrderedLocusNames=RCAP_rcc00680;
OS   Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=272942;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX   PubMed=2747617; DOI=10.1007/bf00334364;
RA   Armstrong G.A., Alberti M., Leach F., Hearst J.E.;
RT   "Nucleotide sequence, organization, and nature of the protein products of
RT   the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus.";
RL   Mol. Gen. Genet. 216:254-268(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX   PubMed=20418398; DOI=10.1128/jb.00366-10;
RA   Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V.,
RA   Haselkorn R.;
RT   "Complete genome sequence of the photosynthetic purple nonsulfur bacterium
RT   Rhodobacter capsulatus SB 1003.";
RL   J. Bacteriol. 192:3545-3546(2010).
CC   -!- FUNCTION: Involved in the biosynthesis of carotenoids. Catalyzes the
CC       condensation of two molecules of geranylgeranyl diphosphate (GGPP) to
CC       give prephytoene diphosphate (PPPP) and the subsequent rearrangement of
CC       the cyclopropylcarbinyl intermediate to yield phytoene (By similarity).
CC       {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=ATP; Xref=ChEBI:CHEBI:30616; Evidence={ECO:0000250};
CC       Note=ATP is required for the transferase activity but it does not seem
CC       to be hydrolyzed during the reaction. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis.
CC   -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC       {ECO:0000305}.
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DR   EMBL; X52291; CAA36534.1; -; Genomic_DNA.
DR   EMBL; Z11165; CAA77541.1; -; Genomic_DNA.
DR   EMBL; CP001312; ADE84445.1; -; Genomic_DNA.
DR   PIR; S04403; S04403.
DR   RefSeq; WP_013066424.1; NC_014034.1.
DR   AlphaFoldDB; P17056; -.
DR   SMR; P17056; -.
DR   STRING; 272942.RCAP_rcc00680; -.
DR   EnsemblBacteria; ADE84445; ADE84445; RCAP_rcc00680.
DR   GeneID; 31489626; -.
DR   KEGG; rcp:RCAP_rcc00680; -.
DR   eggNOG; COG1562; Bacteria.
DR   HOGENOM; CLU_037269_1_0_5; -.
DR   OMA; KLYCYRV; -.
DR   OrthoDB; 1449625at2; -.
DR   BioCyc; MetaCyc:MON-14934; -.
DR   UniPathway; UPA00799; -.
DR   Proteomes; UP000002361; Chromosome.
DR   GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR   GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; ISS:UniProtKB.
DR   CDD; cd00683; Trans_IPPS_HH; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR002060; Squ/phyt_synthse.
DR   InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR   InterPro; IPR044843; Trans_IPPS_bact-type.
DR   InterPro; IPR033904; Trans_IPPS_HH.
DR   Pfam; PF00494; SQS_PSY; 1.
DR   SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
DR   PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR   PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE   3: Inferred from homology;
KW   Carotenoid biosynthesis; Magnesium; Manganese; Metal-binding;
KW   Reference proteome; Transferase.
FT   CHAIN           1..339
FT                   /note="Phytoene synthase"
FT                   /id="PRO_0000067433"
SQ   SEQUENCE   339 AA;  37302 MW;  6D7052994934BF4F CRC64;
     MIAEADMEVC RELIRTGSYS FHAASRVLPA RVRDPALALY AFCRVADDEV DEVGAPRDKA
     AAVLKLGDRL EDIYAGRPRN APSDRAFAAV VEEFEMPREL PEALLEGFAW DAEGRWYHTL
     SDVQAYSARV AAAVGAMMCV LMRVRNPDAL ARACDLGLAM QMSNIARDVG EDARAGRLFL
     PTDWMVEEGI DPQAFLADPQ PTKGIRRVTE RLLNRADRLY WRAATGVRLL PFDCRPGIMA
     AGKIYAAIGA EVAKAKYDNI TRRAHTTKGR KLWLVANSAM SATATSMLPL SPRVHAKPEP
     EVAHLVDAAA HRNLHPERSE VLISALMALK ARDRGLAMD