ID   CRTC_RHOCB              Reviewed;         281 AA.
AC   P17058; D5AP75;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Acyclic carotenoid 1,2-hydratase;
DE            EC=4.2.1.131;
DE   AltName: Full=Hydroxyneurosporene synthase;
DE   AltName: Full=Lycopene hydratase;
DE   AltName: Full=Neurosporene hydratase;
GN   Name=crtC; OrderedLocusNames=RCAP_rcc00682;
OS   Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=272942;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX   PubMed=2747617; DOI=10.1007/bf00334364;
RA   Armstrong G.A., Alberti M., Leach F., Hearst J.E.;
RT   "Nucleotide sequence, organization, and nature of the protein products of
RT   the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus.";
RL   Mol. Gen. Genet. 216:254-268(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX   PubMed=20418398; DOI=10.1128/jb.00366-10;
RA   Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V.,
RA   Haselkorn R.;
RT   "Complete genome sequence of the photosynthetic purple nonsulfur bacterium
RT   Rhodobacter capsulatus SB 1003.";
RL   J. Bacteriol. 192:3545-3546(2010).
RN   [3]
RP   FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBSTRATE
RP   SPECIFICITY.
RX   PubMed=12745254; DOI=10.1016/s0003-9861(03)00099-7;
RA   Steiger S., Mazet A., Sandmann G.;
RT   "Heterologous expression, purification, and enzymatic characterization of
RT   the acyclic carotenoid 1,2-hydratase from Rubrivivax gelatinosus.";
RL   Arch. Biochem. Biophys. 414:51-58(2003).
CC   -!- FUNCTION: Involved in the biosynthesis of carotenoids spheroidene and
CC       spirilloxanthin. Catalyzes the hydration of neurosporene to the
CC       corresponding hydroxylated carotenoids 1-HO-neurosporene and that of
CC       lycopene to 1-HO-lycopene. It exhibits negligible activity in
CC       converting HO-neurosporene, HO-lycopene, or any other derivative such
CC       as spheroidene, 1-HO-3,4-didehydrolycopene.
CC       {ECO:0000269|PubMed:12745254}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=rhodopin = all-trans-lycopene + H2O; Xref=Rhea:RHEA:31607,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15948, ChEBI:CHEBI:35331;
CC         EC=4.2.1.131; Evidence={ECO:0000269|PubMed:12745254};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1,1'-dihydroxy-1,1',2,2'-tetrahydrolycopene = H2O + rhodopin;
CC         Xref=Rhea:RHEA:31611, ChEBI:CHEBI:15377, ChEBI:CHEBI:35331,
CC         ChEBI:CHEBI:63065; EC=4.2.1.131;
CC         Evidence={ECO:0000269|PubMed:12745254};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=22.1 uM for lycopene (at pH 8 and at 28 degrees Celsius)
CC         {ECO:0000269|PubMed:12745254};
CC         KM=24.5 uM for neurosporene (at pH 8 and at 28 degrees Celsius)
CC         {ECO:0000269|PubMed:12745254};
CC         Vmax=0.38 nmol/h/mg enzyme with neurosporene as substrate (at pH 8
CC         and at 28 degrees Celsius) {ECO:0000269|PubMed:12745254};
CC         Vmax=1.46 nmol/h/mg enzyme with lycopene as substrate (at pH 8 and at
CC         28 degrees Celsius) {ECO:0000269|PubMed:12745254};
CC   -!- PATHWAY: Carotenoid biosynthesis; spheroidene biosynthesis.
CC   -!- SIMILARITY: Belongs to the CrtC hydratase family. {ECO:0000305}.
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DR   EMBL; X52291; CAA36536.1; -; Genomic_DNA.
DR   EMBL; Z11165; CAA77543.1; -; Genomic_DNA.
DR   EMBL; CP001312; ADE84447.1; -; Genomic_DNA.
DR   PIR; S04405; S04405.
DR   AlphaFoldDB; P17058; -.
DR   STRING; 272942.RCAP_rcc00682; -.
DR   EnsemblBacteria; ADE84447; ADE84447; RCAP_rcc00682.
DR   KEGG; rcp:RCAP_rcc00682; -.
DR   eggNOG; COG5621; Bacteria.
DR   HOGENOM; CLU_056173_0_0_5; -.
DR   OMA; MLPFRMP; -.
DR   BioCyc; MetaCyc:MON-14930; -.
DR   SABIO-RK; P17058; -.
DR   UniPathway; UPA00683; -.
DR   Proteomes; UP000002361; Chromosome.
DR   GO; GO:0016836; F:hydro-lyase activity; IDA:UniProtKB.
DR   GO; GO:0016116; P:carotenoid metabolic process; IDA:UniProtKB.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   GO; GO:1901180; P:spheroidene biosynthetic process; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; Chlorophyll biosynthesis; Lyase; Photosynthesis;
KW   Reference proteome.
FT   CHAIN           1..281
FT                   /note="Acyclic carotenoid 1,2-hydratase"
FT                   /id="PRO_0000079365"
SQ   SEQUENCE   281 AA;  31857 MW;  3CA97D3B51C33F47 CRC64;
     MIAFIGSVFS PWYRWSGRRE PQNHCCINMV TTGTDGRFTM TDRGRSALRQ SRDSFQVGPS
     KLTWTGKELV IDVDEWGALP KLGKLKGRVV LTPRAVTGVE VRLTPDAGHT WRPFAPIADV
     EVDLAPGHKW TGHGYFDANF GTRALEEDFS FWTWGRFPLK DRTVCFYDAT RLDRTKVALA
     VQINPDGSVC EIDSPPPLVK MKRTPWFVRR ETRCDAGAHP AGVYSLLEAP FYSRALMRTQ
     IDGEETVGMH EALDLVRFRQ PVLKPMLAVR VPRRAGWKHK D