CRTD_RHOCB
ID CRTD_RHOCB Reviewed; 494 AA.
AC P17059; D5AP76;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 2.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Hydroxyneurosporene desaturase;
DE Short=HND;
DE AltName: Full=1-hydroxycarotenoid 3,4-dehydrogenase;
DE AltName: Full=1-hydroxycarotenoid 3,4-desaturase;
DE EC=1.3.99.27;
GN Name=crtD; OrderedLocusNames=RCAP_rcc00683;
OS Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Rhodobacter.
OX NCBI_TaxID=272942;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX PubMed=2747617; DOI=10.1007/bf00334364;
RA Armstrong G.A., Alberti M., Leach F., Hearst J.E.;
RT "Nucleotide sequence, organization, and nature of the protein products of
RT the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus.";
RL Mol. Gen. Genet. 216:254-268(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX PubMed=20418398; DOI=10.1128/jb.00366-10;
RA Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V.,
RA Haselkorn R.;
RT "Complete genome sequence of the photosynthetic purple nonsulfur bacterium
RT Rhodobacter capsulatus SB 1003.";
RL J. Bacteriol. 192:3545-3546(2010).
RN [3]
RP FUNCTION.
RX PubMed=2144293; DOI=10.1016/s0021-9258(18)55500-9;
RA Bartley G.E., Schmidhauser T.J., Yanofsky C., Scolnik P.A.;
RT "Carotenoid desaturases from Rhodobacter capsulatus and Neurospora crassa
RT are structurally and functionally conserved and contain domains homologous
RT to flavoprotein disulfide oxidoreductases.";
RL J. Biol. Chem. 265:16020-16024(1990).
CC -!- FUNCTION: Catalyzes the introduction of C-3,4 double bonds into 1-
CC hydroxyneurosporene (1-HO-Neu) to yield demethylspheroidene (DMS).
CC {ECO:0000305|PubMed:2144293}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=A + rhodopin = (3E)-3,4-didehydrorhodopin + AH2;
CC Xref=Rhea:RHEA:30919, ChEBI:CHEBI:13193, ChEBI:CHEBI:17499,
CC ChEBI:CHEBI:35331, ChEBI:CHEBI:62481; EC=1.3.99.27;
CC -!- PATHWAY: Carotenoid biosynthesis; spheroidene biosynthesis.
CC -!- SIMILARITY: Belongs to the carotenoid/retinoid oxidoreductase family.
CC {ECO:0000305}.
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DR EMBL; X52291; CAA36537.1; -; Genomic_DNA.
DR EMBL; Z11165; CAA77544.1; -; Genomic_DNA.
DR EMBL; CP001312; ADE84448.1; -; Genomic_DNA.
DR PIR; S04406; S04406.
DR RefSeq; WP_013066427.1; NC_014034.1.
DR AlphaFoldDB; P17059; -.
DR SMR; P17059; -.
DR STRING; 272942.RCAP_rcc00683; -.
DR EnsemblBacteria; ADE84448; ADE84448; RCAP_rcc00683.
DR GeneID; 31489629; -.
DR KEGG; rcp:RCAP_rcc00683; -.
DR eggNOG; COG1233; Bacteria.
DR HOGENOM; CLU_019722_2_1_5; -.
DR OMA; FTMRWVF; -.
DR OrthoDB; 418913at2; -.
DR BioCyc; MetaCyc:MON-14932; -.
DR UniPathway; UPA00683; -.
DR Proteomes; UP000002361; Chromosome.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR Gene3D; 3.50.50.60; -; 2.
DR InterPro; IPR002937; Amino_oxidase.
DR InterPro; IPR014105; Carotenoid/retinoid_OxRdtase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR008150; Phytoene_DH_bac_CS.
DR Pfam; PF01593; Amino_oxidase; 2.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR02734; crtI_fam; 1.
DR PROSITE; PS00982; PHYTOENE_DH; 1.
PE 3: Inferred from homology;
KW Carotenoid biosynthesis; Chlorophyll biosynthesis; Oxidoreductase;
KW Photosynthesis; Reference proteome.
FT CHAIN 1..494
FT /note="Hydroxyneurosporene desaturase"
FT /id="PRO_0000067683"
FT CONFLICT 13
FT /note="G -> R (in Ref. 1; CAA77544/CAA36537)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 494 AA; 52213 MW; 300CD1722255C5B4 CRC64;
MRSETDVVVI GAGMGGLAAA IGAAAAGLRV TVVEAGDAPG GKARAVPTPG GPADTGPTVL
TMRHVLDALF AACGTRAEEH LTLIPLPRLA RHFWPDGSSL DLFTDTEANI EAIRAFAGDK
EAAAFRRFDH LTTGLWEAFH RSVIAAPKPD LWRIAAATVT RPQLWPALRP GLTMRDLLAH
HFKDPRLAQL FGRYATYVGG RPGATPAVLS LIWQAEVQGV WAIREGMHGV AAALARVAEA
KGVRFHYGAK AKRIVRKEGR VTAVEIETGV SIPCGACIFN GDPGALRDGL LGDAARASME
KSPRPAPSLS AWVWAFGATP IGVDLAHHNV FFTADPELEF GPIGAGEMPE EPTLYICAQD
REMQAPVPEI ERFEIIMNGP AGHQPFPQEE AQCRARTFPM LAAMGLTFSP DPETRALTTP
ALLSRRFPGS LGAIYGGSPE GTLATFRRPL ARTGLKGLYL AGGGTHPGAG VPMALTSGTH
AARALLADRI SAAK
