CRTEB_CORGL
ID CRTEB_CORGL Reviewed; 287 AA.
AC Q8NSP8; Q6M7E4;
DT 12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Lycopene elongase/hydratase {ECO:0000305};
DE EC=2.5.1.149 {ECO:0000250|UniProtKB:Q93QX2};
GN Name=crtEb {ECO:0000303|PubMed:22963379};
GN OrderedLocusNames=Cgl0620 {ECO:0000312|EMBL:BAB98013.1};
OS Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 /
OS JCM 1318 / LMG 3730 / NCIMB 10025).
OC Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC Corynebacterium.
OX NCBI_TaxID=196627;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=12743753; DOI=10.1007/s00253-003-1328-1;
RA Ikeda M., Nakagawa S.;
RT "The Corynebacterium glutamicum genome: features and impacts on
RT biotechnological processes.";
RL Appl. Microbiol. Biotechnol. 62:99-109(2003).
RN [2]
RP FUNCTION, PATHWAY, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=22963379; DOI=10.1186/1471-2180-12-198;
RA Heider S.A., Peters-Wendisch P., Wendisch V.F.;
RT "Carotenoid biosynthesis and overproduction in Corynebacterium
RT glutamicum.";
RL BMC Microbiol. 12:198-198(2012).
CC -!- FUNCTION: Catalyzes the elongation of the C(40) carotenoid all-trans-
CC lycopene to the acyclic C(50) carotenoid flavuxanthin during
CC decaprenoxanthin biosynthesis (PubMed:22963379). Acts as a bifunctional
CC enzyme that catalyzes the elongation of lycopene by attaching a C(5)
CC isoprene unit at C-2, as well as the hydroxylation of the new isoprene
CC unit. The enzyme acts at both ends of the substrate, forming the C(50)
CC carotenoid flavuxanthin via the C(45) intermediate nonaflavuxanthin (By
CC similarity). {ECO:0000250|UniProtKB:Q93QX2,
CC ECO:0000269|PubMed:22963379}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=A + all-trans-lycopene + dimethylallyl diphosphate + H2O = AH2
CC + diphosphate + nonaflavuxanthin; Xref=Rhea:RHEA:56124,
CC ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:15948,
CC ChEBI:CHEBI:17499, ChEBI:CHEBI:33019, ChEBI:CHEBI:57623,
CC ChEBI:CHEBI:139514; EC=2.5.1.149;
CC Evidence={ECO:0000250|UniProtKB:Q93QX2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=A + dimethylallyl diphosphate + H2O + nonaflavuxanthin = AH2 +
CC diphosphate + flavuxanthin; Xref=Rhea:RHEA:56128, ChEBI:CHEBI:13193,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:17499, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:57623, ChEBI:CHEBI:139514, ChEBI:CHEBI:139515;
CC EC=2.5.1.149; Evidence={ECO:0000250|UniProtKB:Q93QX2};
CC -!- PATHWAY: Carotenoid biosynthesis. {ECO:0000269|PubMed:22963379}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Mutant accumulates lycopene and cannot produce
CC neither flavuxanthin nor decaprenoxanthin.
CC {ECO:0000269|PubMed:22963379}.
CC -!- SIMILARITY: Belongs to the UbiA prenyltransferase family.
CC {ECO:0000305}.
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DR EMBL; BA000036; BAB98013.1; -; Genomic_DNA.
DR RefSeq; NP_599855.1; NC_003450.3.
DR RefSeq; WP_011013770.1; NC_006958.1.
DR AlphaFoldDB; Q8NSP8; -.
DR STRING; 196627.cg0717; -.
DR KEGG; cgl:Cgl0620; -.
DR PATRIC; fig|196627.13.peg.610; -.
DR eggNOG; COG0382; Bacteria.
DR HOGENOM; CLU_058976_1_0_11; -.
DR OMA; PANVFLY; -.
DR Proteomes; UP000000582; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016765; F:transferase activity, transferring alkyl or aryl (other than methyl) groups; IEA:InterPro.
DR Gene3D; 1.10.357.140; -; 1.
DR InterPro; IPR000537; UbiA_prenyltransferase.
DR InterPro; IPR044878; UbiA_sf.
DR Pfam; PF01040; UbiA; 1.
PE 3: Inferred from homology;
KW Cell membrane; Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..287
FT /note="Lycopene elongase/hydratase"
FT /id="PRO_0000450584"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 37..57
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 287 AA; 31705 MW; E16831C77874C2CA CRC64;
MMEKIRLILL SSRPISWINT AYPFGLAYLL NAGEIDWLFW LGIVFFLIPY NIAMYGINDV
FDYESDMRNP RKGGVEGAVL PKSSHSTLLW ASAISTIPFL VILFIFGTWM SSLWLTLSVL
AVIAYSAPKL RFKERPFIDA LTSSTHFTSP ALIGATITGT SPSAAMWIAL GSFFLWGMAS
QILGAVQDVN ADREANLSSI ATVIGARGAI RLSVVLYLLA AVLVTTLPNP AWIIGIAILT
YVFNAARFWN ITDASCEQAN RSWKVFLWLN YFVGAVITIL LIAIHQI
