CRTE_CERS4
ID CRTE_CERS4 Reviewed; 288 AA.
AC P54976; Q3J195; Q9RFC5;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 3.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Geranylgeranyl diphosphate synthase;
DE Short=GGPP synthase;
DE EC=2.5.1.29;
DE AltName: Full=Farnesyltranstransferase;
GN Name=crtE; OrderedLocusNames=RHOS4_18710; ORFNames=RSP_0265;
OS Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG
OS 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) (Rhodobacter
OS sphaeroides).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Cereibacter.
OX NCBI_TaxID=272943;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7721699; DOI=10.1128/jb.177.8.2064-2073.1995;
RA Lang H.P., Cogdell R.J., Takaichi S., Hunter C.N.;
RT "Complete DNA sequence, specific Tn5 insertion map, and gene assignment of
RT the carotenoid biosynthesis pathway of Rhodobacter sphaeroides.";
RL J. Bacteriol. 177:2064-2073(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10648776; DOI=10.1093/nar/28.4.862;
RA Choudhary M., Kaplan S.;
RT "DNA sequence analysis of the photosynthesis region of Rhodobacter
RT sphaeroides 2.4.1.";
RL Nucleic Acids Res. 28:862-867(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203
RC / NCIMB 8253 / ATH 2.4.1.;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C.,
RA Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.;
RT "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1.";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the condensation of farnesyl diphosphate (FPP) and
CC isopentenyl diphosphate (IPP) to yield geranylgeranyl diphosphate
CC (GGPP) needed for biosynthesis of carotenoids and diterpenes.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate =
CC (2E,6E,10E)-geranylgeranyl diphosphate + diphosphate;
CC Xref=Rhea:RHEA:17653, ChEBI:CHEBI:33019, ChEBI:CHEBI:58756,
CC ChEBI:CHEBI:128769, ChEBI:CHEBI:175763; EC=2.5.1.29;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 2 Mg(2+) ions per subunit. {ECO:0000250};
CC -!- PATHWAY: Isoprenoid biosynthesis; geranylgeranyl diphosphate
CC biosynthesis; geranylgeranyl diphosphate from farnesyl diphosphate and
CC isopentenyl diphosphate: step 1/1.
CC -!- SIMILARITY: Belongs to the FPP/GGPP synthase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABA79439.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AJ010302; CAB38744.1; -; Genomic_DNA.
DR EMBL; AF195122; AAF24294.1; -; Genomic_DNA.
DR EMBL; CP000143; ABA79439.2; ALT_INIT; Genomic_DNA.
DR PIR; S49625; S49625.
DR PIR; T50750; T50750.
DR RefSeq; WP_011338112.1; NZ_AKVW01000001.1.
DR RefSeq; YP_353340.2; NC_007493.2.
DR AlphaFoldDB; P54976; -.
DR SMR; P54976; -.
DR STRING; 272943.RSP_0265; -.
DR EnsemblBacteria; ABA79439; ABA79439; RSP_0265.
DR KEGG; rsp:RSP_0265; -.
DR PATRIC; fig|272943.9.peg.2209; -.
DR eggNOG; COG0142; Bacteria.
DR PhylomeDB; P54976; -.
DR UniPathway; UPA00389; UER00564.
DR Proteomes; UP000002703; Chromosome 1.
DR GO; GO:0004311; F:farnesyltranstransferase activity; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0033386; P:geranylgeranyl diphosphate biosynthetic process; ISS:UniProtKB.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR CDD; cd00685; Trans_IPPS_HT; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR000092; Polyprenyl_synt.
DR InterPro; IPR033749; Polyprenyl_synt_CS.
DR Pfam; PF00348; polyprenyl_synt; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
DR PROSITE; PS00723; POLYPRENYL_SYNTHASE_1; 1.
DR PROSITE; PS00444; POLYPRENYL_SYNTHASE_2; 1.
PE 3: Inferred from homology;
KW Carotenoid biosynthesis; Chlorophyll biosynthesis; Isoprene biosynthesis;
KW Magnesium; Metal-binding; Photosynthesis; Reference proteome; Transferase.
FT CHAIN 1..288
FT /note="Geranylgeranyl diphosphate synthase"
FT /id="PRO_0000123995"
FT BINDING 43
FT /ligand="isopentenyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:128769"
FT /evidence="ECO:0000250|UniProtKB:P14324"
FT BINDING 73
FT /ligand="isopentenyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:128769"
FT /evidence="ECO:0000250|UniProtKB:Q12051"
FT BINDING 80
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P14324"
FT BINDING 80
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:P14324"
FT BINDING 86
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P14324"
FT BINDING 86
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:P14324"
FT BINDING 91
FT /ligand="(2E,6E)-farnesyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:175763"
FT /evidence="ECO:0000250"
FT BINDING 92
FT /ligand="isopentenyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:128769"
FT /evidence="ECO:0000250|UniProtKB:P14324"
FT BINDING 170
FT /ligand="(2E,6E)-farnesyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:175763"
FT /evidence="ECO:0000250"
FT BINDING 171
FT /ligand="(2E,6E)-farnesyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:175763"
FT /evidence="ECO:0000250"
FT BINDING 205
FT /ligand="(2E,6E)-farnesyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:175763"
FT /evidence="ECO:0000250"
FT CONFLICT 53
FT /note="A -> R (in Ref. 1; CAB38744)"
FT /evidence="ECO:0000305"
FT CONFLICT 57
FT /note="D -> S (in Ref. 1; CAB38744)"
FT /evidence="ECO:0000305"
FT CONFLICT 60
FT /note="A -> V (in Ref. 2; AAF24294)"
FT /evidence="ECO:0000305"
FT CONFLICT 123
FT /note="R -> G (in Ref. 1; CAB38744)"
FT /evidence="ECO:0000305"
FT CONFLICT 128
FT /note="Q -> R (in Ref. 1; CAB38744)"
FT /evidence="ECO:0000305"
FT CONFLICT 234
FT /note="N -> S (in Ref. 1; CAB38744)"
FT /evidence="ECO:0000305"
FT CONFLICT 247
FT /note="R -> G (in Ref. 1; CAB38744)"
FT /evidence="ECO:0000305"
FT CONFLICT 264
FT /note="G -> A (in Ref. 1; CAB38744)"
FT /evidence="ECO:0000305"
FT CONFLICT 277
FT /note="E -> D (in Ref. 1; CAB38744)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 288 AA; 30229 MW; FAB8D98C8557A8A2 CRC64;
MAFEQRIEAA MAAAIARGQG SEAPSKLATA LDYAVTPGGA RIRPTLLLSV ATACGDDRPA
LSDAAAVALE LIHCASLVHD DLPCFDDAEI RRGKPTVHRA YSEPLAILTG DSLIVMGFEV
LARAAADQPQ RALQLVTALA VRTGMPMGIC AGQGWESESQ INLSAYHRAK TGALFIAATQ
MGAIAAGYEA EPWEELGARI GEAFQVADDL RDALCDAETL GKPAGQDEIH ARPNAVREYG
VEGAAKRLKD ILGGAIASIP SCPGEAMLAE MVRRYAEKIV PAQVAARV
