CRTE_PANAN
ID CRTE_PANAN Reviewed; 302 AA.
AC P21684;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1991, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Geranylgeranyl diphosphate synthase;
DE Short=GGPP synthase;
DE EC=2.5.1.29;
DE AltName: Full=Farnesyltranstransferase;
GN Name=crtE;
OS Pantoea ananas (Erwinia uredovora).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Pantoea.
OX NCBI_TaxID=553;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=ATCC 19321 / DSM 30080 / NCPPB 800 / NRRL B-14773 / 20D3;
RX PubMed=2254247; DOI=10.1128/jb.172.12.6704-6712.1990;
RA Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K.,
RA Harashima K.;
RT "Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by
RT functional analysis of gene products expressed in Escherichia coli.";
RL J. Bacteriol. 172:6704-6712(1990).
CC -!- FUNCTION: Catalyzes the condensation of farnesyl diphosphate (FPP) and
CC isopentenyl diphosphate (IPP) to yield geranylgeranyl diphosphate
CC (GGPP) needed for biosynthesis of carotenoids and diterpenes.
CC {ECO:0000305|PubMed:2254247}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate =
CC (2E,6E,10E)-geranylgeranyl diphosphate + diphosphate;
CC Xref=Rhea:RHEA:17653, ChEBI:CHEBI:33019, ChEBI:CHEBI:58756,
CC ChEBI:CHEBI:128769, ChEBI:CHEBI:175763; EC=2.5.1.29;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 2 Mg(2+) ions per subunit. {ECO:0000250};
CC -!- PATHWAY: Isoprenoid biosynthesis; geranylgeranyl diphosphate
CC biosynthesis; geranylgeranyl diphosphate from farnesyl diphosphate and
CC isopentenyl diphosphate: step 1/1.
CC -!- SIMILARITY: Belongs to the FPP/GGPP synthase family. {ECO:0000305}.
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DR EMBL; D90087; BAA14124.1; -; Genomic_DNA.
DR PIR; A37802; A37802.
DR AlphaFoldDB; P21684; -.
DR SMR; P21684; -.
DR UniPathway; UPA00389; UER00564.
DR GO; GO:0004311; F:farnesyltranstransferase activity; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0033386; P:geranylgeranyl diphosphate biosynthetic process; IDA:UniProtKB.
DR CDD; cd00685; Trans_IPPS_HT; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR000092; Polyprenyl_synt.
DR InterPro; IPR033749; Polyprenyl_synt_CS.
DR Pfam; PF00348; polyprenyl_synt; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
DR PROSITE; PS00723; POLYPRENYL_SYNTHASE_1; 1.
DR PROSITE; PS00444; POLYPRENYL_SYNTHASE_2; 1.
PE 3: Inferred from homology;
KW Carotenoid biosynthesis; Isoprene biosynthesis; Magnesium; Metal-binding;
KW Transferase.
FT CHAIN 1..302
FT /note="Geranylgeranyl diphosphate synthase"
FT /id="PRO_0000123993"
FT BINDING 53
FT /ligand="isopentenyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:128769"
FT /evidence="ECO:0000250|UniProtKB:P14324"
FT BINDING 56
FT /ligand="isopentenyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:128769"
FT /evidence="ECO:0000250|UniProtKB:P14324"
FT BINDING 87
FT /ligand="isopentenyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:128769"
FT /evidence="ECO:0000250|UniProtKB:Q12051"
FT BINDING 94
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P14324"
FT BINDING 94
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:P14324"
FT BINDING 100
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P14324"
FT BINDING 100
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:P14324"
FT BINDING 105
FT /ligand="(2E,6E)-farnesyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:175763"
FT /evidence="ECO:0000250"
FT BINDING 106
FT /ligand="isopentenyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:128769"
FT /evidence="ECO:0000250|UniProtKB:P14324"
FT BINDING 189
FT /ligand="(2E,6E)-farnesyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:175763"
FT /evidence="ECO:0000250"
FT BINDING 190
FT /ligand="(2E,6E)-farnesyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:175763"
FT /evidence="ECO:0000250"
FT BINDING 227
FT /ligand="(2E,6E)-farnesyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:175763"
FT /evidence="ECO:0000250"
SQ SEQUENCE 302 AA; 32583 MW; CADB04699D2EBA4A CRC64;
MTVCAKKHVH LTRDAAEQLL ADIDRRLDQL LPVEGERDVV GAAMREGALA PGKRIRPMLL
LLTARDLGCA VSHDGLLDLA CAVEMVHAAS LILDDMPCMD DAKLRRGRPT IHSHYGEHVA
ILAAVALLSK AFGVIADADG LTPLAKNRAV SELSNAIGMQ GLVQGQFKDL SEGDKPRSAE
AILMTNHFKT STLFCASMQM ASIVANASSE ARDCLHRFSL DLGQAFQLLD DLTDGMTDTG
KDSNQDAGKS TLVNLLGPRA VEERLRQHLQ LASEHLSAAC QHGHATQHFI QAWFDKKLAA
VS
