CRTF_DICDI
ID CRTF_DICDI Reviewed; 876 AA.
AC Q54YU6;
DT 15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=CAR1 transcription factor;
DE Short=CRTF;
DE AltName: Full=CAR1-binding factor;
GN Name=CRTF; ORFNames=DDB_G0278077;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=9716534; DOI=10.1242/dev.125.18.3689;
RA Mu X., Lee B., Louis J.M., Kimmel A.R.;
RT "Sequence-specific protein interaction with a transcriptional enhancer
RT involved in the autoregulated expression of cAMP receptor 1 in
RT Dictyostelium.";
RL Development 125:3689-3698(1998).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=11493573; DOI=10.1242/dev.128.13.2569;
RA Mu X., Spanos S.A., Shiloach J., Kimmel A.;
RT "CRTF is a novel transcription factor that regulates multiple stages of
RT Dictyostelium development.";
RL Development 128:2569-2579(2001).
RN [4]
RP INDUCTION [LARGE SCALE ANALYSIS].
RX PubMed=18590548; DOI=10.1186/1471-2180-8-109;
RA Carilla-Latorre S., Calvo-Garrido J., Bloomfield G., Skelton J., Kay R.R.,
RA Ivens A., Martinez J.L., Escalante R.;
RT "Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common
RT and specific effects from PAO1 and PA14 strains.";
RL BMC Microbiol. 8:109-109(2008).
CC -!- FUNCTION: Nuclear transcription factor with zinc-dependent sequence
CC binding specificity. Required for carA-1 and carA-2 expression in early
CC development. Also required throughout later developmental stages
CC - where it has a cell-autonomous role that is distinct from its
CC capacity to control carA-1 and carA-2 expression. Required for terminal
CC spore differentiation but not essential for cell differentiation and
CC cell sorting. May function to help insulate distinct pathways from
CC simultaneous and universal activation by cAMP.
CC {ECO:0000269|PubMed:11493573, ECO:0000269|PubMed:9716534}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11493573,
CC ECO:0000269|PubMed:9716534}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development.
CC {ECO:0000269|PubMed:11493573, ECO:0000269|PubMed:9716534}.
CC -!- INDUCTION: Up-regulated by P.aeruginosa, PAO1 strain and PA14 strain
CC infection. {ECO:0000269|PubMed:18590548}.
CC -!- DISRUPTION PHENOTYPE: Failure to develop under standard conditions and
CC failure to induce expression of carA-1 and carA-2 early in development.
CC Failure to sporulate, even under conditions that bypass the dependence
CC on early cAMP signaling pathways. Do not form mature spores and all
CC cells of the mutant spore mass are dark in phase with round or
CC irregular shape. These cells are fragile and easily disrupted under a
CC glass cover slip. Very low level of expression of carA-1 and carA-2
CC with resulting defects in cAMP signaling and thus impaired aggregation,
CC although aggregates develop normally in the presence of a wild-type
CC strain. Post-aggregation and sporulation defects are cell autonomous
CC and independent of carA-1 expression. Early developmental events of
CC CRTF-null strain can be rescued with exogenous cAMP treatment,
CC constitutive carA-1 expression or co-development with wild-type cells;
CC however, these treatments are insufficient to promote sporulation. In
CC shaking culture, null cells grow with the same doubling time as wild-
CC type cells and form normal size growth plaques on bacterial lawns;
CC however, development within these plaques is very poor. When cells are
CC developed directly on solid substrata, development of mutant cells is
CC completely blocked prior to aggregation. {ECO:0000269|PubMed:11493573}.
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DR EMBL; AAFI02000023; EAL68211.1; -; Genomic_DNA.
DR RefSeq; XP_642106.1; XM_637014.1.
DR AlphaFoldDB; Q54YU6; -.
DR SMR; Q54YU6; -.
DR STRING; 44689.DDB0216392; -.
DR PaxDb; Q54YU6; -.
DR EnsemblProtists; EAL68211; EAL68211; DDB_G0278077.
DR GeneID; 8621317; -.
DR KEGG; ddi:DDB_G0278077; -.
DR dictyBase; DDB_G0278077; crtf.
DR eggNOG; ENOG502RETY; Eukaryota.
DR HOGENOM; CLU_328299_0_0_1; -.
DR OMA; STISHWE; -.
DR PRO; PR:Q54YU6; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005634; C:nucleus; IDA:dictyBase.
DR GO; GO:0003677; F:DNA binding; IDA:dictyBase.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:dictyBase.
DR GO; GO:0043938; P:positive regulation of sporulation; IMP:dictyBase.
DR GO; GO:0010043; P:response to zinc ion; IDA:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IMP:dictyBase.
DR GO; GO:0006351; P:transcription, DNA-templated; IDA:dictyBase.
PE 2: Evidence at transcript level;
KW Coiled coil; Developmental protein; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation; Zinc.
FT CHAIN 1..876
FT /note="CAR1 transcription factor"
FT /id="PRO_0000390407"
FT REGION 76..120
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 199..336
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 360..395
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 409..478
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 530..552
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 565..643
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 654..870
FT /note="Interaction with the carA promoter element"
FT COILED 483..524
FT /evidence="ECO:0000255"
FT COMPBIAS 568..620
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 876 AA; 98085 MW; 9FB2B7E1BE65FE10 CRC64;
MSSIEQLYST TQFPSLSSCN TNINNTNNNN NINNINNNTN INTGNLLKNT FFKNNIIYNT
NNNSINNNSN NNIINNNNNN LLNSNNQNSF QNQNNLNSIH NHNNNNNNNN SNNNNTSNSE
NLDSLKSALL SSNIFNGSSS SIAHQNLINL IISSQNNNNN NGNSNSNTPD QKSTMADILL
NSSVPDYFEW CSDIINMPKD GNEKSNNSNG GNNNGNNNGN NNNNHNNNNN NNSNNNNNNN
SDSVPSPQNN YQSPQSDNQS SMVPSFNFSN QNSANSSNNN NNNNGSSNNN NNVRSEPTTP
NDESNNNSNN TNNNNNNNNN NNNNQQQQLS SNQNSSPLIY SVPSYYVPNY GVVLSAPMKG
NSSSSILPPQ AWPQNNNNNN GNSNSNNGNN NASGFSDFMF NGSNIDYNSI MNNNNNNNNN
NNNNNNNNNN NNNNSNNNNN NSNNNNGNNG GMFNGGFVNN NNHNNNNNNN NNNGNLNFHN
FLQFQNQLQN SNQQQQQQQL QQQQQLQQHQ QQQQQLQQQQ LQQQFNIGKP CNINQKKGRG
ANNNNNNMPI GNQASLVISH PSLKMEGHHH LQQQQQQQHH HQHQQHQQNG NGNNTQIVNS
AFLDLSSPDS HNQMSPSSPI CLPESPMGAQ HWESGLDPSS LQKQNEINPP ISVRSNVVFF
EPYLAGEPGR HKACWNLLEP MHRNYYQPIQ FKLPSFPDTS LPITQIDDKT GIFDSQRFLA
FNNPQAMSKY ESYRIYIHPS LGYSGNAKRF KQQPDVNEKA LILDGNVYDG HLNPIYNCKI
CTEYYQTKSY FSANPHAKGK VLLVKNNILT RVKDGGFTLS LKPMCCSGHN SHIPLYFHFT
LTNPLTNEVV LQSLINVNVK QWKKSVPNKS KKQRFE
