ID   CRTF_RHOCA              Reviewed;          77 AA.
AC   P0CY89; P17061;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 32.
DE   RecName: Full=Demethylspheroidene O-methyltransferase;
DE            EC=2.1.1.210;
DE   AltName: Full=Hydroxyneurosporene methyltransferase;
DE   Flags: Fragment;
GN   Name=crtF;
OS   Rhodobacter capsulatus (Rhodopseudomonas capsulata).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=1061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2550757; DOI=10.1007/bf00330558;
RA   Young D.A., Bauer C.E., Williams J.C., Marrs B.L.;
RT   "Genetic evidence for superoperonal organization of genes for
RT   photosynthetic pigments and pigment-binding proteins in Rhodobacter
RT   capsulatus.";
RL   Mol. Gen. Genet. 218:1-12(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 72-77.
RC   STRAIN=ATCC 33303 / B10;
RX   PubMed=2555268; DOI=10.1016/0378-1119(89)90111-x;
RA   Wellington C.L., Beatty J.T.;
RT   "Promoter mapping and nucleotide sequence of the bchC bacteriochlorophyll
RT   biosynthesis gene from Rhodobacter capsulatus.";
RL   Gene 83:251-261(1989).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=7358679; DOI=10.1016/s0021-9258(19)85909-4;
RA   Scolnik P.A., Walker M.A., Marrs B.L.;
RT   "Biosynthesis of carotenoids derived from neurosporene in Rhodopseudomonas
RT   capsulata.";
RL   J. Biol. Chem. 255:2427-2432(1980).
RN   [4]
RP   FUNCTION.
RX   PubMed=12664193; DOI=10.1007/s00203-003-0538-3;
RA   Pinta V., Ouchane S., Picaud M., Takaichi S., Astier C., Reiss-Husson F.;
RT   "Characterization of unusual hydroxy- and ketocarotenoids in Rubrivivax
RT   gelatinosus: involvement of enzyme CrtF or CrtA.";
RL   Arch. Microbiol. 179:354-362(2003).
CC   -!- FUNCTION: Methyltransferase that mediates the O-methylation of 1-
CC       hydroxy carotenoids. Converts hydroxyneurosporene to
CC       methoxyneurosporene or demethylspheroidene to spheroidene. Also able to
CC       produce spirilloxanthin. {ECO:0000269|PubMed:12664193,
CC       ECO:0000269|PubMed:7358679}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=demethylspheroidene + S-adenosyl-L-methionine = H(+) + S-
CC         adenosyl-L-homocysteine + spheroidene; Xref=Rhea:RHEA:30903,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:35330, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:62505; EC=2.1.1.210;
CC   -!- PATHWAY: Carotenoid biosynthesis; spheroidene biosynthesis.
CC   -!- DISRUPTION PHENOTYPE: Cells accumulate demethylspheroidene as their
CC       major carotenoid during anaerobic growth and demethylspheroidenone when
CC       grown in the presence of oxygen. {ECO:0000269|PubMed:7358679}.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. Cation-independent O-methyltransferase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU01020}.
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DR   EMBL; X16164; CAA34289.1; -; Genomic_DNA.
DR   EMBL; M29966; AAA26095.1; -; Genomic_DNA.
DR   PIR; S04408; S04408.
DR   AlphaFoldDB; P0CY89; -.
DR   SMR; P0CY89; -.
DR   BioCyc; MetaCyc:MON-14933; -.
DR   UniPathway; UPA00683; -.
DR   GO; GO:0043803; F:hydroxyneurosporene-O-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008171; F:O-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR016461; COMT-like.
DR   InterPro; IPR001077; O_MeTrfase_dom.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF00891; Methyltransf_2; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   3: Inferred from homology;
KW   Carotenoid biosynthesis; Chlorophyll biosynthesis; Methyltransferase;
KW   Photosynthesis; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..77
FT                   /note="Demethylspheroidene O-methyltransferase"
FT                   /id="PRO_0000079367"
FT   NON_TER         1
SQ   SEQUENCE   77 AA;  8394 MW;  654121351E5F092D CRC64;
     ALPPGGRLII SEAMAGGAKP DRACDVYFAF YTMAMSSGRT RSPEEIKQML EKAGFTKVSK
     PRTLRPFITS VIEAERG