CRTI_CERNC
ID CRTI_CERNC Reviewed; 621 AA.
AC P48537;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=Phytoene desaturase;
DE EC=1.3.99.30;
DE AltName: Full=Phytoene desaturase (3,4-didehydrolycopene-forming);
DE Flags: Precursor;
GN Name=PDH1;
OS Cercospora nicotianae (Barn spot disease fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Cercospora.
OX NCBI_TaxID=29003;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 18366;
RX PubMed=8085820; DOI=10.1128/aem.60.8.2766-2771.1994;
RA Ehrenshaft M., Daub M.E.;
RT "Isolation, sequence, and characterization of the Cercospora nicotianae
RT phytoene dehydrogenase gene.";
RL Appl. Environ. Microbiol. 60:2766-2771(1994).
CC -!- FUNCTION: Phytoene desaturase involved in the carotenoid biosynthesis
CC pathway. Converts phytoene into 3,4-didehydrolycopene via the
CC intermediary of phytofluene, zeta-carotene, neurosporene and lycopene,
CC by introducing up to five double bonds into phytoene (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=15-cis-phytoene + 5 A = 5 AH2 + all-trans-3,4-
CC didehydrolycopene; Xref=Rhea:RHEA:30975, ChEBI:CHEBI:13193,
CC ChEBI:CHEBI:17499, ChEBI:CHEBI:27787, ChEBI:CHEBI:62474;
CC EC=1.3.99.30;
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC -!- PATHWAY: Carotenoid biosynthesis; lycopene biosynthesis.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the carotenoid/retinoid oxidoreductase family.
CC {ECO:0000305}.
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DR EMBL; U03903; AAB86988.1; -; Genomic_DNA.
DR PIR; T48646; T48646.
DR AlphaFoldDB; P48537; -.
DR SMR; P48537; -.
DR UniPathway; UPA00803; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.50.50.60; -; 2.
DR InterPro; IPR002937; Amino_oxidase.
DR InterPro; IPR014105; Carotenoid/retinoid_OxRdtase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR008150; Phytoene_DH_bac_CS.
DR Pfam; PF01593; Amino_oxidase; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR02734; crtI_fam; 1.
DR PROSITE; PS00982; PHYTOENE_DH; 1.
PE 3: Inferred from homology;
KW Carotenoid biosynthesis; Membrane; NAD; Oxidoreductase; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..621
FT /note="Phytoene desaturase"
FT /id="PRO_0000067694"
FT TRANSMEM 598..618
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 394..425
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 399..420
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 621 AA; 69530 MW; 61B9EA7784963CA8 CRC64;
MPSTSKRPTA IVIGSGVGGV STAARLARAG FHVTVLEKNN FTGGRCSLIH HEGYRFDQGP
SLLLLPGLFH RTFAELGTSL EQEGVKLLKC EPNYMIHFSD GEKFTLSSDL SVMKTEVEKW
EGKEGYTRYL EFLKESHGHY ELSVREVLLR NFEGLTAMLR PEFLRHLLQL HPFESIWTRA
GKYFWTERLR RVFTFGSMYM GMSPFDAPGT YSLLQYTELA EGIWYPVGGF HRVVEALVKI
GEREGVDFRM ETAVKKILLS EDGGVAKGVE LEDGRRLEAD VVVNNSDLVY AYEKLLPIKT
PYAESLKGRP GSCSSISFYW ALDRQVPELE AHNIFLADEY RESFDSIFKK HLIPDEPSFY
VNVPSRVDST AAPEGKDSVV VLVPVGHLLE EDRHASQAHQ LSASRNGHIS SASPPDQPGL
TPTEKQDWPA MISLARKTIL STIQSRTNVD LTPLIIHEST NSPLSWKQTF NLDRGAILGL
SHSFFNVLCF RPTTRARKPG AFDAQLLKFG VLGRAAEVII DAFRGRGKDI KGLYMVGASA
HPGTGVPICL AGGALVAEQI CGDYGVDIPW KEEERKGRDV GGKRKLDVLE SPMWLDSWEQ
WVSVLIYLLV GIFAWLWMKF R
