2SS_FAGTA
ID 2SS_FAGTA Reviewed; 149 AA.
AC E9NX73;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 1.
DT 03-AUG-2022, entry version 23.
DE RecName: Full=2S seed storage albumin protein {ECO:0000305};
DE AltName: Full=16 kDa allergen Fag t 2 {ECO:0000303|PubMed:33412487};
DE AltName: Full=16 kDa major allergen {ECO:0000303|PubMed:21645012, ECO:0000303|PubMed:29306152, ECO:0000312|EMBL:ADW27428.1};
DE AltName: Full=2S albumin {ECO:0000303|PubMed:21645012, ECO:0000303|PubMed:29306152};
DE AltName: Full=2S seed storage protein {ECO:0000305};
DE AltName: Full=Major allergen Fag t 2 {ECO:0000303|PubMed:21645012};
DE AltName: Allergen=Fag t 2.0101 {ECO:0000305};
DE Flags: Precursor;
OS Fagopyrum tataricum (Tartarian buckwheat) (Polygonum tataricum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Polygonaceae; Polygonoideae; Fagopyreae; Fagopyrum.
OX NCBI_TaxID=62330 {ECO:0000312|EMBL:ADW27428.1};
RN [1] {ECO:0000312|EMBL:ADW27428.1}
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 23-34, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND ALLERGEN.
RC TISSUE=Seed {ECO:0000303|PubMed:21645012};
RX PubMed=21645012; DOI=10.1111/j.1398-9995.2011.02657.x;
RA Chen P., Guo Y.F., Yan Q., Li Y.H.;
RT "Molecular cloning and characterization of Fag t 2: a 16-kDa major allergen
RT from Tartary buckwheat seeds.";
RL Allergy 66:1393-1395(2011).
RN [2]
RP PROTEIN SEQUENCE OF 23-34, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP IDENTIFICATION BY MASS SPECTROMETRY, ALLERGEN, 3D-STRUCTURE MODELING, AND
RP PHYLOGENETIC ANALYSIS.
RX PubMed=29306152; DOI=10.1016/j.molimm.2017.12.024;
RA Zheng B., Zhang H., Wang L., Guo Y., Chen P.;
RT "Characterization of 16-kDa major allergen with alpha-amylase inhibitor
RT domain in tartary buckwheat seeds.";
RL Mol. Immunol. 94:121-130(2018).
RN [3]
RP ALLERGEN, REGIONS, MUTAGENESIS OF CYS-38; CYS-52; ARG-71; 87-CYS-CYS-88;
RP CYS-100 AND LYS-132, 3D-STRUCTURE MODELING, AND CIRCULAR DICHROISM
RP ANALYSIS.
RX PubMed=33412487; DOI=10.1016/j.foodchem.2020.128953;
RA Zheng B., Zhang H., Shen W., Wang L., Chen P.;
RT "Core epitope analysis of 16 kDa allergen from tartary buckwheat.";
RL Food Chem. 346:128953-128953(2021).
CC -!- FUNCTION: Seed storage protein. {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in seeds (at protein level)
CC (PubMed:21645012, PubMed:29306152). Expressed in seeds
CC (PubMed:29306152). {ECO:0000269|PubMed:21645012,
CC ECO:0000269|PubMed:29306152}.
CC -!- DEVELOPMENTAL STAGE: Expressed in developing seeds. Expressed in
CC embryo, but not in endosperm (at protein level).
CC {ECO:0000269|PubMed:21645012, ECO:0000269|PubMed:29306152}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE of
CC patients allergic to buckwheat (PubMed:21645012, PubMed:29306152,
CC PubMed:33412487). Binds to IgE in 74% of the 23 tartarian buckwheat-
CC allergic patients tested (PubMed:29306152). IgE-binding of the natural
CC and recombinant protein is unaffected by heat treatment or pepsin
CC digestion (PubMed:21645012, PubMed:29306152).
CC {ECO:0000269|PubMed:21645012, ECO:0000269|PubMed:29306152,
CC ECO:0000269|PubMed:33412487}.
CC -!- SIMILARITY: Belongs to the 2S seed storage albumins family.
CC {ECO:0000303|PubMed:21645012, ECO:0000303|PubMed:29306152}.
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DR EMBL; HQ829975; ADW27428.1; -; mRNA.
DR Allergome; 9488; Fag t 2.
DR Allergome; 9511; Fag t 2.0101.
DR GO; GO:0019863; F:IgE binding; IEA:UniProtKB-KW.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR000617; Napin/2SS/CON.
DR PANTHER; PTHR35496; PTHR35496; 1.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
PE 1: Evidence at protein level;
KW Allergen; Direct protein sequencing; Disulfide bond; IgE-binding protein;
KW Seed storage protein; Signal; Storage protein.
FT SIGNAL 1..22
FT /evidence="ECO:0000269|PubMed:21645012,
FT ECO:0000269|PubMed:29306152"
FT CHAIN 23..149
FT /note="2S seed storage albumin protein"
FT /evidence="ECO:0000305|PubMed:21645012,
FT ECO:0000305|PubMed:29306152"
FT /id="PRO_5003243888"
FT REGION 41..53
FT /note="No IgE-binding"
FT /evidence="ECO:0000269|PubMed:33412487"
FT REGION 68..81
FT /note="No IgE-binding"
FT /evidence="ECO:0000269|PubMed:33412487"
FT REGION 84..95
FT /note="No IgE-binding"
FT /evidence="ECO:0000269|PubMed:33412487"
FT REGION 97..105
FT /note="No IgE-binding"
FT /evidence="ECO:0000269|PubMed:33412487"
FT REGION 108..117
FT /note="IgE-binding"
FT /evidence="ECO:0000269|PubMed:33412487"
FT REGION 121..131
FT /note="No IgE-binding"
FT /evidence="ECO:0000269|PubMed:33412487"
FT REGION 132..141
FT /note="IgE-binding"
FT /evidence="ECO:0000269|PubMed:33412487"
FT DISULFID 38..98
FT /evidence="ECO:0000250|UniProtKB:Q647G9"
FT DISULFID 52..87
FT /evidence="ECO:0000250|UniProtKB:Q647G9"
FT DISULFID 88..133
FT /evidence="ECO:0000250|UniProtKB:Q647G9"
FT DISULFID 100..140
FT /evidence="ECO:0000250|UniProtKB:Q647G9"
FT MUTAGEN 38
FT /note="C->G: No effect in a competitive IgE inhibition
FT ELISA."
FT /evidence="ECO:0000269|PubMed:33412487"
FT MUTAGEN 52
FT /note="C->G: 21% inhibitory efficiency in a competitive IgE
FT inhibition ELISA."
FT /evidence="ECO:0000269|PubMed:33412487"
FT MUTAGEN 71
FT /note="R->N: No effect in a competitive IgE inhibition
FT ELISA."
FT /evidence="ECO:0000269|PubMed:33412487"
FT MUTAGEN 87..88
FT /note="CC->GG: 33% inhibitory efficiency in a competitive
FT IgE inhibition ELISA."
FT /evidence="ECO:0000269|PubMed:33412487"
FT MUTAGEN 100
FT /note="C->Y: 43% inhibitory efficiency in a competitive IgE
FT inhibition ELISA."
FT /evidence="ECO:0000269|PubMed:33412487"
FT MUTAGEN 132
FT /note="K->N: 55% inhibitory efficiency in a competitive IgE
FT inhibition ELISA."
FT /evidence="ECO:0000269|PubMed:33412487"
SQ SEQUENCE 149 AA; 17280 MW; AAC1B76E740AA260 CRC64;
MKLFIILATA TLLIAATQAK YLRDEGFDLG ETQMSSKCTR QVKMMEPELV KCNRYIAMDI
MDDKYEEALS RIQGEGCESE EKFLRGCCVA MKEMEDECVC EWMKMMVENQ KGRIGETLMR
KGIRDLKELP NKCGISEMEC HSRGNWYYV
