CRTI_CERS4
ID CRTI_CERS4 Reviewed; 518 AA.
AC P54980; Q3J190; Q9RFD0;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Phytoene desaturase (neurosporene-forming);
DE EC=1.3.99.28;
DE AltName: Full=3-step phytoene desaturase;
DE AltName: Full=Phytoene dehydrogenase;
GN Name=crtI; OrderedLocusNames=RHOS4_18760; ORFNames=RSP_0271;
OS Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG
OS 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) (Rhodobacter
OS sphaeroides).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Cereibacter.
OX NCBI_TaxID=272943;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203
RC / NCIMB 8253 / ATH 2.4.1.;
RX PubMed=8021167; DOI=10.1128/jb.176.13.3859-3869.1994;
RA Lang H.P., Cogdell R.J., Gardiner A.T., Hunter C.N.;
RT "Early steps in carotenoid biosynthesis: sequences and transcriptional
RT analysis of the crtI and crtB genes of Rhodobacter sphaeroides and
RT overexpression and reactivation of crtI in Escherichia coli and R.
RT sphaeroides.";
RL J. Bacteriol. 176:3859-3869(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7721699; DOI=10.1128/jb.177.8.2064-2073.1995;
RA Lang H.P., Cogdell R.J., Takaichi S., Hunter C.N.;
RT "Complete DNA sequence, specific Tn5 insertion map, and gene assignment of
RT the carotenoid biosynthesis pathway of Rhodobacter sphaeroides.";
RL J. Bacteriol. 177:2064-2073(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10648776; DOI=10.1093/nar/28.4.862;
RA Choudhary M., Kaplan S.;
RT "DNA sequence analysis of the photosynthesis region of Rhodobacter
RT sphaeroides 2.4.1.";
RL Nucleic Acids Res. 28:862-867(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203
RC / NCIMB 8253 / ATH 2.4.1.;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C.,
RA Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.;
RT "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1.";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Converts phytoene into all-trans-neurosporene as the major
CC product, via the intermediary of phytofluene and zeta-carotene, by the
CC introduction of three double bonds. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=15-cis-phytoene + 3 A = 3 AH2 + all-trans-neurosporene;
CC Xref=Rhea:RHEA:30599, ChEBI:CHEBI:13193, ChEBI:CHEBI:16833,
CC ChEBI:CHEBI:17499, ChEBI:CHEBI:27787; EC=1.3.99.28;
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC -!- PATHWAY: Carotenoid biosynthesis.
CC -!- SIMILARITY: Belongs to the carotenoid/retinoid oxidoreductase family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; S71770; AAB31138.1; -; Genomic_DNA.
DR EMBL; AJ010302; CAB38739.1; -; Genomic_DNA.
DR EMBL; AF195122; AAF24289.1; -; Genomic_DNA.
DR EMBL; CP000143; ABA79444.1; -; Genomic_DNA.
DR PIR; S49620; S49620.
DR PIR; T50745; T50745.
DR RefSeq; WP_002720435.1; NZ_CP030271.1.
DR RefSeq; YP_353345.1; NC_007493.2.
DR AlphaFoldDB; P54980; -.
DR SMR; P54980; -.
DR STRING; 272943.RSP_0271; -.
DR EnsemblBacteria; ABA79444; ABA79444; RSP_0271.
DR GeneID; 57470589; -.
DR KEGG; rsp:RSP_0271; -.
DR PATRIC; fig|272943.9.peg.2214; -.
DR eggNOG; COG1233; Bacteria.
DR OMA; INYPKGG; -.
DR PhylomeDB; P54980; -.
DR BRENDA; 1.3.99.28; 5383.
DR Proteomes; UP000002703; Chromosome 1.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.50.50.60; -; 2.
DR InterPro; IPR002937; Amino_oxidase.
DR InterPro; IPR014105; Carotenoid/retinoid_OxRdtase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR008150; Phytoene_DH_bac_CS.
DR Pfam; PF01593; Amino_oxidase; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR02734; crtI_fam; 1.
DR PROSITE; PS00982; PHYTOENE_DH; 1.
PE 3: Inferred from homology;
KW Carotenoid biosynthesis; FAD; Flavoprotein; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..518
FT /note="Phytoene desaturase (neurosporene-forming)"
FT /id="PRO_0000067691"
FT BINDING 14..47
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255"
FT CONFLICT 73
FT /note="R -> C (in Ref. 3; AAF24289)"
FT /evidence="ECO:0000305"
FT CONFLICT 174
FT /note="S -> T (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 292
FT /note="L -> F (in Ref. 3; AAF24289)"
FT /evidence="ECO:0000305"
FT CONFLICT 395
FT /note="Q -> P (in Ref. 3; AAF24289)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 518 AA; 57231 MW; 6D85BAA8CCD62CBB CRC64;
MPSISPASDA DRALVIGSGL GGLAAAMRLG AKGWRVTVID KLDVPGGRGS SITQEGHRFD
LGPTIVTVPQ SLRDLWKTCG RDFDADVELK PIDPFYEVRW PDGSHFTVRQ STEAMKAEVA
RLSPGDVAGY EKFLKDSEKR YWFGYEDLGR RSMHKLWDLI KVLPTFGMMR ADRSVYQHAA
LRVKDERLRM ALSFHPLFIG GDPFNVTSMY ILVSQLEKEF GVHYAIGGVA AIAAAMAKVI
EGQGGSFRMN TEVDEILVEK GTATGVRLAS GEVLRAGLVV SNADAGHTYM RLLRNHPRRR
WTDAHVKSRR WSMGLFVWYF GTKGTKGMWP DVGHHTIVNA PRYKGLVEDI FLKGKLAKDM
SLYIHRPSIT DPTVAPEGDD TFYALSPVPH LKQAQPVDWQ AVAEPYRESV LEVLEQSMPG
IGERIGPSLV FTPETFRDRY LSPWGAGFSI EPRILQSAWF RPHNISEEVA NLFLVGAGTH
PGAGVPGVIG SAEVMAKLAP DAPRARREAE PAERLAAE
