ID   CRTI_MYXXA              Reviewed;         529 AA.
AC   Q02861;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=All-trans-zeta-carotene desaturase;
DE            EC=1.3.99.26;
DE   AltName: Full=Phytoene dehydrogenase;
DE   AltName: Full=Phytoene desaturase;
GN   Name=carC; Synonyms=crtIb;
OS   Myxococcus xanthus.
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Myxococcaceae; Myxococcus.
OX   NCBI_TaxID=34;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MR403;
RX   PubMed=8467787; DOI=10.1002/j.1460-2075.1993.tb05771.x;
RA   Fontes M., Ruiz-Vazquez R.M., Murillo F.J.;
RT   "Growth phase dependence of the activation of a bacterial gene for
RT   carotenoid synthesis by blue light.";
RL   EMBO J. 12:1265-1275(1993).
RN   [2]
RP   FUNCTION, PATHWAY, AND CATALYTIC ACTIVITY.
RX   PubMed=17662111; DOI=10.1111/j.1742-4658.2007.05960.x;
RA   Iniesta A.A., Cervantes M., Murillo F.J.;
RT   "Cooperation of two carotene desaturases in the production of lycopene in
RT   Myxococcus xanthus.";
RL   FEBS J. 274:4306-4314(2007).
CC   -!- FUNCTION: Dehydrogenates carotenes in the trans conformation: converts
CC       all-trans-zeta-carotene into all-trans-lycopene, one of the last
CC       dehydrogenation steps of lycopene biosynthesis.
CC       {ECO:0000269|PubMed:17662111}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 A + all-trans-zeta-carotene = 2 AH2 + all-trans-lycopene;
CC         Xref=Rhea:RHEA:30619, ChEBI:CHEBI:13193, ChEBI:CHEBI:15948,
CC         ChEBI:CHEBI:17499, ChEBI:CHEBI:28068; EC=1.3.99.26;
CC         Evidence={ECO:0000269|PubMed:17662111};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000305};
CC   -!- PATHWAY: Carotenoid biosynthesis; lycopene biosynthesis.
CC       {ECO:0000269|PubMed:17662111}.
CC   -!- INDUCTION: By blue light.
CC   -!- SIMILARITY: Belongs to the carotenoid/retinoid oxidoreductase family.
CC       {ECO:0000305}.
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DR   EMBL; M94727; AAA25390.1; -; Genomic_DNA.
DR   PIR; S35306; S35306.
DR   AlphaFoldDB; Q02861; -.
DR   SMR; Q02861; -.
DR   PRIDE; Q02861; -.
DR   BioCyc; MetaCyc:MON-16371; -.
DR   UniPathway; UPA00803; -.
DR   GO; GO:0016627; F:oxidoreductase activity, acting on the CH-CH group of donors; IDA:UniProtKB.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IDA:UniProtKB.
DR   Gene3D; 3.50.50.60; -; 2.
DR   InterPro; IPR002937; Amino_oxidase.
DR   InterPro; IPR014105; Carotenoid/retinoid_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR008150; Phytoene_DH_bac_CS.
DR   Pfam; PF01593; Amino_oxidase; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR02734; crtI_fam; 1.
DR   PROSITE; PS00982; PHYTOENE_DH; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; FAD; Flavoprotein; NAD; Oxidoreductase.
FT   CHAIN           1..529
FT                   /note="All-trans-zeta-carotene desaturase"
FT                   /id="PRO_0000067688"
FT   BINDING         12..45
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   529 AA;  58421 MW;  53536A8DFD0D24BC CRC64;
     MASEGGSVRH VIVVGAGPGG LSAAINLAGQ GFRVTVVEKD AVPGGRMKGL TLGASGEYAV
     DTGPSILQLP GVLEQIFRRA ARRLEDYVKL LPLDVNTRVH FWDGTHLDTT RHLDRMEAEL
     AKFGPRQASA LRQWMEDGRE KYGIAYQKFI CTSADNLGYY APWRLAPTLR FKPWQTLYRQ
     LDGFFHDDRV TYALAYPSKY LGLHPTTCSS VFSVIPFLEL AFGVWHVEGG FRELSRGMMR
     CARDLGATFR MGTPVEKVRV DAGRAVGVKL VGGEVLDADA VVVNADLAYA ARSLIPAEAR
     EGSRLTDAAL ERAKYSCSTF MAYYGLDTVY ADLPHHLIYL SESARRTDRD ALEDRHVDLE
     DPPFYVCNPG VTDPSGAPAG HSTLYVLVPT PNTGRPVDWV KTEQALRERI PAMLEKVGLK
     GVREHIREER YFTAETWRDD FNVFRGAVFN LSHTWLQLGP LRPKVKNRDI EGLYFVGGGT
     HPGSGLLTIM ESANIAADYL TREAGKGPLP GWPYVPPLEP ESPVQARAG