CRTI_PHYB8
ID CRTI_PHYB8 Reviewed; 583 AA.
AC P54982;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=Phytoene desaturase;
DE EC=1.3.99.30;
DE AltName: Full=Phytoene desaturase (3,4-didehydrolycopene-forming);
DE Flags: Precursor;
GN Name=carB;
OS Phycomyces blakesleeanus (strain ATCC 8743b / DSM 1359 / FGSC 10004 / NBRC
OS 33097 / NRRL 1555).
OC Eukaryota; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina;
OC Mucoromycetes; Mucorales; Phycomycetaceae; Phycomyces.
OX NCBI_TaxID=763407;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 8743b / DSM 1359 / FGSC 10004 / NBRC 33097 / NRRL 1555;
RX PubMed=9079885; DOI=10.1007/s004380050378;
RA Ruiz-Hidalgo M.J., Benito E.P., Sandmann G., Eslava A.P.;
RT "The phytoene dehydrogenase gene of Phycomyces: regulation of its
RT expression by blue light and vitamin A.";
RL Mol. Gen. Genet. 253:734-744(1997).
CC -!- FUNCTION: Phytoene desaturase involved in the carotenoid biosynthesis
CC pathway. Converts phytoene into 3,4-didehydrolycopene via the
CC intermediary of phytofluene, zeta-carotene, neurosporene and lycopene,
CC by introducing up to five double bonds into phytoene (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=15-cis-phytoene + 5 A = 5 AH2 + all-trans-3,4-
CC didehydrolycopene; Xref=Rhea:RHEA:30975, ChEBI:CHEBI:13193,
CC ChEBI:CHEBI:17499, ChEBI:CHEBI:27787, ChEBI:CHEBI:62474;
CC EC=1.3.99.30;
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC -!- PATHWAY: Carotenoid biosynthesis; lycopene biosynthesis.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the carotenoid/retinoid oxidoreductase family.
CC {ECO:0000305}.
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DR EMBL; X78434; CAA55197.1; -; Genomic_DNA.
DR PIR; S43139; S43139.
DR RefSeq; XP_018294564.1; XM_018440097.1.
DR AlphaFoldDB; P54982; -.
DR SMR; P54982; -.
DR GeneID; 29001003; -.
DR VEuPathDB; FungiDB:PHYBL_37852; -.
DR UniPathway; UPA00803; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.50.50.60; -; 2.
DR InterPro; IPR002937; Amino_oxidase.
DR InterPro; IPR014105; Carotenoid/retinoid_OxRdtase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR008150; Phytoene_DH_bac_CS.
DR Pfam; PF01593; Amino_oxidase; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR02734; crtI_fam; 1.
DR PROSITE; PS00982; PHYTOENE_DH; 1.
PE 3: Inferred from homology;
KW Carotenoid biosynthesis; Membrane; NAD; Oxidoreductase; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..583
FT /note="Phytoene desaturase"
FT /id="PRO_0000067696"
FT TRANSMEM 531..551
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 583 AA; 65984 MW; B0E8F682B12FB591 CRC64;
MAPPKHVIII GAGAGGTATA ARLAREGIKV TVVEKNNFGG GRCSLINHNG HRFDQGPSLY
LMPKLFEEAF EALDEKIEDH VELLRCHNNY KVHFDDGDKI QLSSDLSRMK PEMERIEGPD
GFLRFLDFMK ESHTHYEGGV EMAIKQNFET IWKLIRLQYV PALFRLHIFD FVYSRAAKYF
KTKKMRMAFT FQSMYMGMSP YDSPAVYNLL QYTEFAEGIW YPKGGFNTVI QKLENIATEK
FGARFIYEAP VAKINTDDKG KKVTGVTLQS GEVIEADAVV CNADLVYAYH NLLPPCRWTT
NTLAEKKLTS SSISFYWSLK RVVPELDVHN IFLAEAFKES FDEIFTDHKM PSELSFYVNL
PSRIDPTAAP PGKDSMIVLV PIGHMKSKTN EAEDYTMIVK RARKMVLEVL ERRLGLTNFI
DLVEHEEVND PSIWQKKFNL WRGSILGLSH DVLQVLWFRP STQDSTGRYK NLFFVGASTH
PGTGVPIVLA GSKLTSDQVC DHFGVKVRPS AITSSKRTYA PEDSKSFIWD IIWFLLIALF
AATLVLFIAF PQYSEVNQTA ASYINNLLPA AFRVPVANLS LTS
