ID   CRTI_PSEVU              Reviewed;         492 AA.
AC   P22871;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Phytoene desaturase (lycopene-forming);
DE            EC=1.3.99.31;
DE   AltName: Full=4-step phytoene desaturase;
DE   AltName: Full=Phytoene dehydrogenase;
GN   Name=crtI;
OS   Pseudescherichia vulneris (Escherichia vulneris).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Pseudescherichia.
OX   NCBI_TaxID=566;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 39368 / Eho10;
RX   PubMed=2263648; DOI=10.1073/pnas.87.24.9975;
RA   Armstrong G.A., Alberti M., Hearst J.E.;
RT   "Conserved enzymes mediate the early reactions of carotenoid biosynthesis
RT   in nonphotosynthetic and photosynthetic prokaryotes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:9975-9979(1990).
CC   -!- FUNCTION: This enzyme converts phytoene into lycopene via the
CC       intermediaries of phytofluene, zeta-carotene and neurosporene by the
CC       introduction of four double bonds.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=15-cis-phytoene + 4 A = 4 AH2 + all-trans-lycopene;
CC         Xref=Rhea:RHEA:15585, ChEBI:CHEBI:13193, ChEBI:CHEBI:15948,
CC         ChEBI:CHEBI:17499, ChEBI:CHEBI:27787; EC=1.3.99.31;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000305};
CC   -!- PATHWAY: Carotenoid biosynthesis; lycopene biosynthesis.
CC   -!- SIMILARITY: Belongs to the carotenoid/retinoid oxidoreductase family.
CC       {ECO:0000305}.
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DR   EMBL; M38423; AAA24820.1; -; Genomic_DNA.
DR   EMBL; M87280; AAA64981.1; -; Genomic_DNA.
DR   PIR; A39273; A33120.
DR   AlphaFoldDB; P22871; -.
DR   SMR; P22871; -.
DR   PRIDE; P22871; -.
DR   UniPathway; UPA00803; -.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 3.
DR   InterPro; IPR002937; Amino_oxidase.
DR   InterPro; IPR014105; Carotenoid/retinoid_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR008150; Phytoene_DH_bac_CS.
DR   Pfam; PF01593; Amino_oxidase; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR02734; crtI_fam; 1.
DR   PROSITE; PS00982; PHYTOENE_DH; 1.
PE   3: Inferred from homology;
KW   Carotenoid biosynthesis; FAD; Flavoprotein; Oxidoreductase.
FT   CHAIN           1..492
FT                   /note="Phytoene desaturase (lycopene-forming)"
FT                   /id="PRO_0000067685"
FT   BINDING         5..38
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   492 AA;  54503 MW;  8EDC5DB1562083F2 CRC64;
     MKKTVVIGAG FGGLALAIRL QAAGIPTVLL EQRDKPGGRA YVWHDQGFTF DAGPTVITDP
     TALEALFTLA GRRMEDYVRL LPVKPFYRLC WESGKTLDYA NDSAELEAQI TQFNPRDVEG
     YRRFLAYSQA VFQEGYLRLG SVPFLSFRDM LRAGPQLLKL QAWQSVYQSV SRFIEDEHLR
     QAFSFHSLLV GGNPFTTSSI YTLIHALERE WGVWFPEGGT GALVNGMVKL FTDLGGEIEL
     NARVEELVVA DNRVSQVRLA DGRIFDTDAV ASNADVVNTY KKLLGHHPVG QKRAAALERK
     SMSNSLFVLY FGLNQPHSQL AHHTICFGPR YRELIDEIFT GSALADDFSL YLHSPCVTDP
     SLAPPGCASF YVLAPVPHLG NAPLDWAQEG PKLRDRIFDY LEERYMPGLR SQLVTQRIFT
     PADFHDTLDA HLGSAFSIEP LLTQSAWFRP HNRDSDIANL YLVGAGTHPG AGIPGVVASA
     KATASLMIED LQ