ID   CRTI_SYNY3              Reviewed;         472 AA.
AC   P29273;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   03-AUG-2022, entry version 133.
DE   RecName: Full=Phytoene dehydrogenase;
DE            EC=1.3.99.-;
DE   AltName: Full=Phytoene desaturase;
GN   Name=pds; Synonyms=crtD; OrderedLocusNames=slr1254;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC   unclassified Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1581575; DOI=10.1007/bf00019213;
RA   Martinez-Ferez I.M., Vioque A.;
RT   "Nucleotide sequence of the phytoene desaturase gene from Synechocystis sp.
RT   PCC 6803 and characterization of a new mutation which confers resistance to
RT   the herbicide norflurazon.";
RL   Plant Mol. Biol. 18:981-983(1992).
RN   [2]
RP   SEQUENCE REVISION TO C-TERMINUS.
RA   Vioque A.;
RL   Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
CC   -!- FUNCTION: This enzyme converts phytoene into zeta-carotene via the
CC       intermediary of phytofluene by the symmetrical introduction of two
CC       double bonds at the C-11 and C-11' positions of phytoene.
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000305};
CC       Name=NADP(+); Xref=ChEBI:CHEBI:58349; Evidence={ECO:0000305};
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000305};
CC   -!- ACTIVITY REGULATION: Inhibited by the herbicide norflurazon in a non-
CC       competitive way.
CC   -!- PATHWAY: Carotenoid biosynthesis; lycopene biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Peripheral membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the carotenoid/retinoid oxidoreductase family.
CC       {ECO:0000305}.
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DR   EMBL; X62574; CAA44452.1; -; Genomic_DNA.
DR   EMBL; BA000022; BAA17847.1; -; Genomic_DNA.
DR   PIR; S74886; S74886.
DR   AlphaFoldDB; P29273; -.
DR   SMR; P29273; -.
DR   IntAct; P29273; 3.
DR   STRING; 1148.1652929; -.
DR   PaxDb; P29273; -.
DR   EnsemblBacteria; BAA17847; BAA17847; BAA17847.
DR   KEGG; syn:slr1254; -.
DR   eggNOG; COG0654; Bacteria.
DR   eggNOG; COG3349; Bacteria.
DR   InParanoid; P29273; -.
DR   OMA; HSMIFNQ; -.
DR   PhylomeDB; P29273; -.
DR   UniPathway; UPA00803; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR   GO; GO:0016166; F:phytoene dehydrogenase activity; IEA:InterPro.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0009635; P:response to herbicide; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR002937; Amino_oxidase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR001613; Flavin_amine_oxidase.
DR   InterPro; IPR014102; Phytoene_desaturase.
DR   Pfam; PF01593; Amino_oxidase; 1.
DR   PRINTS; PR00757; AMINEOXDASEF.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR02731; phytoene_desat; 1.
PE   3: Inferred from homology;
KW   Carotenoid biosynthesis; Cell membrane; FAD; Flavoprotein;
KW   Herbicide resistance; Membrane; NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..472
FT                   /note="Phytoene dehydrogenase"
FT                   /id="PRO_0000067698"
FT   BINDING         7..23
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
FT   VARIANT         195
FT                   /note="R -> C (confers resistance to the herbicide
FT                   norflurazon)"
SQ   SEQUENCE   472 AA;  52921 MW;  81D089A6DAA28758 CRC64;
     MRVVIAGAGL AGLACAKYLA DAGFTPVVLE RRDVLGGKIA AWKDEDGDWY ETGLHIFFGA
     YPNMLQLFKE LDIEDRLQWK EHSMIFNQPE KPGTYSRFDF PDIPAPINGL VAILRNNDML
     TWPEKIRFGL GLLPAIVQGQ SYVEEMDKYT WSEWMAKQNI PPRIEKEVFI AMSKALNFID
     PDEISATILL TALNRFLQEK NGSKMAFLDG APPERLCQPL VDYITERGGE VHINKPLKEI
     LLNEDGSVKG YLIRGLDGAP DEVITADLYV SAMPVDPLKT MVPAPWREYP EFKQIQGLEG
     VPVINLHLWF DRKLTDIDHL LFSRSPLLSV YADMSNTCRE YSDPDKSMLE LVLAPAQDWI
     GKSDEEIVAA TMAEIKQLFP QHFNGDNPAR LLKSHVVKTP RSVYKATPGR QACRPDQRTS
     VPNFYLAGDF TMQKYLGSME GAVLSGKQCA QAIAADFNPQ TVPPTREIVT VG