ID   CRTJ_MYXXA              Reviewed;         517 AA.
AC   P54979;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=zeta-carotene-forming phytoene desaturase;
DE            EC=1.3.99.29;
DE   AltName: Full=2-step phytoene desaturase;
GN   Name=carA2; Synonyms=crtIa;
OS   Myxococcus xanthus.
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Myxococcaceae; Myxococcus.
OX   NCBI_TaxID=34;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=DK1050;
RX   PubMed=7588751; DOI=10.1111/j.1432-1033.1995.238_1.x;
RA   Botella J.A., Murillo F.J., Ruiz-Vazquez R.M.;
RT   "A cluster of structural and regulatory genes for light-induced
RT   carotenogenesis in Myxococcus xanthus.";
RL   Eur. J. Biochem. 233:238-248(1995).
RN   [2]
RP   FUNCTION, PATHWAY, AND CATALYTIC ACTIVITY.
RX   PubMed=17662111; DOI=10.1111/j.1742-4658.2007.05960.x;
RA   Iniesta A.A., Cervantes M., Murillo F.J.;
RT   "Cooperation of two carotene desaturases in the production of lycopene in
RT   Myxococcus xanthus.";
RL   FEBS J. 274:4306-4314(2007).
CC   -!- FUNCTION: Dehydrogenates carotenes in the cis conformation: has cis-to-
CC       trans isomerase activity and mediates dehydrogenation of cis-phytoene,
CC       producing zeta-carotene via the intermediary of phytofluene by the
CC       symmetrical introduction of 2 double bonds at the C-11 and C-11'
CC       positions of phytoene. {ECO:0000269|PubMed:17662111,
CC       ECO:0000269|PubMed:7588751}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=15-cis-phytoene + 2 A = 2 AH2 + all-trans-zeta-carotene;
CC         Xref=Rhea:RHEA:30615, ChEBI:CHEBI:13193, ChEBI:CHEBI:17499,
CC         ChEBI:CHEBI:27787, ChEBI:CHEBI:28068; EC=1.3.99.29;
CC         Evidence={ECO:0000269|PubMed:17662111};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000305};
CC   -!- PATHWAY: Carotenoid biosynthesis; lycopene biosynthesis.
CC       {ECO:0000269|PubMed:17662111}.
CC   -!- SIMILARITY: Belongs to the carotenoid/retinoid oxidoreductase family.
CC       {ECO:0000305}.
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DR   EMBL; Z21955; CAA79956.1; -; Genomic_DNA.
DR   PIR; S32169; S32169.
DR   AlphaFoldDB; P54979; -.
DR   SMR; P54979; -.
DR   BioCyc; MetaCyc:MON-16379; -.
DR   UniPathway; UPA00803; -.
DR   GO; GO:0016627; F:oxidoreductase activity, acting on the CH-CH group of donors; IDA:UniProtKB.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IDA:UniProtKB.
DR   Gene3D; 3.50.50.60; -; 2.
DR   InterPro; IPR002937; Amino_oxidase.
DR   InterPro; IPR014105; Carotenoid/retinoid_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR008150; Phytoene_DH_bac_CS.
DR   Pfam; PF01593; Amino_oxidase; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR02734; crtI_fam; 1.
DR   PROSITE; PS00982; PHYTOENE_DH; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; FAD; Flavoprotein; NAD; Oxidoreductase.
FT   CHAIN           1..517
FT                   /note="zeta-carotene-forming phytoene desaturase"
FT                   /id="PRO_0000067689"
FT   BINDING         11..44
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   517 AA;  57784 MW;  CECDD74AB9F9F8CB CRC64;
     MSASTQGRRI VVVGAGVGGL AAAARLAHQG FDVQVFEKTQ GPGGRCNRLQ VDGFTWDLGP
     TIVLMPEVFE ETFRAVGRRI EDYLTLLRCD PNYRVHFRDR SDVTFTSELC AMGRELERVE
     PGSYARYLAF LAQGRVQYRT SLDHLVGRNY AGLRDYLSPR VLARIFQVRA HRRMYADVSR
     FFQDERLRAA MTFQTMYLGV SPYASPAVYG LLPFTELGVG IWFPKGGLYA IPQALERLAR
     EEGVRFHYGA PVERILTDGG RTRGVRLEGG EVVEADAVLC NADLPYAYEK LLDPKATTLK
     RKEKLRYTSS GYMLYLGMKR RYPELLHHNV VFGRDYKGSF DDIFEFRVPE DPSFYVNAPT
     RTDASLAPEG KDALYVLVPV PHQHPDLDWK VEGPKVRAKF FARMAELGFP SLESDIEVER
     RSSTPDDWAG TFNLARGSGF GLSQNFTQIG PFRPSNQDAR VKNLFFVGAS TQPGTGLPTV
     LISARLVTER LMTWAHAQGV SLSPRTAAAT PLEGVAA