ID   CRTNC_METID             Reviewed;         491 AA.
AC   P0DPE9;
DT   28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT   28-MAR-2018, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=4,4'-diapolycopen-4-al dehydrogenase {ECO:0000305};
DE            EC=1.2.99.10 {ECO:0000269|PubMed:25326460};
DE   AltName: Full=4,4'-diapolycopen-4-al oxidase {ECO:0000305};
DE   AltName: Full=4,4'-diapolycopen-4-oate synthase {ECO:0000305};
DE   AltName: Full=4,4'-diapolycopene aldehyde oxidase {ECO:0000303|PubMed:25326460};
GN   Name=crtNc {ECO:0000303|PubMed:25326460};
OS   Metabacillus indicus (Bacillus indicus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Metabacillus.
OX   NCBI_TaxID=246786;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=HU36;
RX   PubMed=25326460; DOI=10.1099/mic.0.083519-0;
RA   Steiger S., Perez-Fons L., Cutting S.M., Fraser P.D., Sandmann G.;
RT   "Annotation and functional assignment of the genes for the C30 carotenoid
RT   pathways from the genomes of two bacteria: Bacillus indicus and Bacillus
RT   firmus.";
RL   Microbiology 161:194-202(2015).
CC   -!- FUNCTION: Involved in the biosynthesis of the major C30 carotenoid
CC       methyl 4'-[6-O-(acylglycosyl)oxy]-4,4'-diapolycopen-4-oic acid via
CC       4,4'-diapolycopen-4-oic acid intermediate. Catalyzes the oxidation of
CC       4,4'-diapolycopen-4-al to yield 4,4'-diapolycopen-4-oic acid.
CC       {ECO:0000269|PubMed:25326460}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=A + all-trans-4,4'-diapolycopen-4-al + H2O = AH2 + all-trans-
CC         4,4'-diapolycopen-4-oate + H(+); Xref=Rhea:RHEA:44716,
CC         ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17499, ChEBI:CHEBI:138599, ChEBI:CHEBI:138600;
CC         EC=1.2.99.10; Evidence={ECO:0000269|PubMed:25326460};
CC   -!- PATHWAY: Carotenoid biosynthesis. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the carotenoid/retinoid oxidoreductase family.
CC       CrtN subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0DPE9; -.
DR   SMR; P0DPE9; -.
DR   STRING; 246786.GS18_0221725; -.
DR   BRENDA; 1.2.99.10; 14726.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 2.
DR   InterPro; IPR002937; Amino_oxidase.
DR   InterPro; IPR014105; Carotenoid/retinoid_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   Pfam; PF01593; Amino_oxidase; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR02734; crtI_fam; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; Oxidoreductase.
FT   CHAIN           1..491
FT                   /note="4,4'-diapolycopen-4-al dehydrogenase"
FT                   /id="PRO_0000443510"
FT   ACT_SITE        208
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10007"
SQ   SEQUENCE   491 AA;  54648 MW;  E4674AEC00208916 CRC64;
     MKIAIAGGGI GGLISALMLR KKGYEVSLFE KRDRLGGRLA FTEEQGYRID EGPTIVLLPE
     MLTSILNEAG ISRDQYELIN INPLYKLHFK DGSSYTKYNS IERQIQEIKE NFPGNEEGFV
     QFMKDMEIRF NLGKDQFLEK SFHDKRTFWT RNNLKTLVHL KAYKSVNNSL KAYFQDERIR
     QAYSLQTLYI GGNPLDSPAL YSLISFSEHK HGIYYLKGGY ASLVTVLENA LLNSGVKVMK
     NATVERVVTE GEQAAALIVN GEEVKADAFV LNGDFPGASK MIPKESMPAR NYTASSSCVL
     LYFGLDKVYR DSPVHQFFMG SNFQQHMKEI FETKEVPSDP SIYAFHPSVI DSSLAPEGHG
     VLYALVPVPS GSPINWGEQE GFVEKVIDQL EERGFPGLRK SIQWKKVRTP DDKEMEGLFQ
     GGSFGIAPTL FQSGVFRPQV KPSKLTNVYA AGASIHPGGG IPIVMQGAKL MVSAILSDHQ
     NKEREGVSLS G