ID   CRTN_METSP              Reviewed;         511 AA.
AC   Q4VKV1;
DT   28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=4,4'-diapophytoene desaturase (4,4'-diapolycopene-forming) {ECO:0000305};
DE            EC=1.3.8.2 {ECO:0000305|PubMed:15933032};
DE   AltName: Full=4,4'-diapophytoene desaturase {ECO:0000303|PubMed:15933032};
DE   AltName: Full=Dehydrosqualene desaturase {ECO:0000305};
GN   Name=crtN {ECO:0000312|EMBL:AAX46183.1};
OS   Methylomonas sp.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales;
OC   Methylococcaceae; Methylomonas.
OX   NCBI_TaxID=418;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, DISRUPTION
RP   PHENOTYPE, AND PATHWAY.
RC   STRAIN=16a {ECO:0000312|EMBL:AAX46183.1};
RX   PubMed=15933032; DOI=10.1128/aem.71.6.3294-3301.2005;
RA   Tao L., Schenzle A., Odom J.M., Cheng Q.;
RT   "Novel carotenoid oxidase involved in biosynthesis of 4,4'-diapolycopene
RT   dialdehyde.";
RL   Appl. Environ. Microbiol. 71:3294-3301(2005).
CC   -!- FUNCTION: Involved in the biosynthesis of C30 carotenoids. Catalyzes
CC       four successive dehydrogenation reactions that lead to the introduction
CC       of four double bonds into 4,4'-diapophytoene (dehydrosqualene) to yield
CC       4,4'-diapolycopene. {ECO:0000269|PubMed:15933032}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=15-cis-4,4'-diapophytoene + 4 FAD + 4 H(+) = all-trans-4,4'-
CC         diapolycopene + 4 FADH2; Xref=Rhea:RHEA:31391, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57692, ChEBI:CHEBI:58307, ChEBI:CHEBI:62449,
CC         ChEBI:CHEBI:62738; EC=1.3.8.2;
CC         Evidence={ECO:0000305|PubMed:15933032};
CC   -!- PATHWAY: Carotenoid biosynthesis. {ECO:0000305|PubMed:15933032}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene accumulate the colorless
CC       C30 carotenoid precursor 4,4'-diapophytoene.
CC       {ECO:0000269|PubMed:15933032}.
CC   -!- SIMILARITY: Belongs to the carotenoid/retinoid oxidoreductase family.
CC       {ECO:0000305}.
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DR   EMBL; AY841893; AAX46183.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q4VKV1; -.
DR   SMR; Q4VKV1; -.
DR   BioCyc; MetaCyc:MON-16325; -.
DR   GO; GO:0102223; F:4,4'-diapophytoene desaturase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 2.
DR   InterPro; IPR002937; Amino_oxidase.
DR   InterPro; IPR014105; Carotenoid/retinoid_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR008150; Phytoene_DH_bac_CS.
DR   Pfam; PF01593; Amino_oxidase; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR02734; crtI_fam; 1.
DR   PROSITE; PS00982; PHYTOENE_DH; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; FAD; Flavoprotein; Oxidoreductase.
FT   CHAIN           1..511
FT                   /note="4,4'-diapophytoene desaturase (4,4'-diapolycopene-
FT                   forming)"
FT                   /id="PRO_0000443501"
SQ   SEQUENCE   511 AA;  58116 MW;  B4F7C2ED1F7066E9 CRC64;
     MANTKHIIIV GAGPGGLCAG MLLSQRGFKV SIFDKHAEIG GRNRPINMNG FTFDTGPTFL
     LMKGVLDEMF ELCERRSEDY LEFLPLSPMY RLLYDDRDIF VYSDRENMRA ELQRVFDEGT
     DGYEQFMEQE RKRFNALYPC ITRDYSSLKS FLSLDLIKAL PWLAFPKSVF NNLGQYFNQE
     KMRLAFCFQS KYLGMSPWEC PALFTMLPYL EHEYGIYHVK GGLNRIAAAM AQVIAENGGE
     IHLNSEIESL IIENGAAKGV KLQHGAELRG DEVIINADFA HAMTHLVKPG VLKKYTPENL
     KQREYSCSTF MLYLGLDKIY DLPHHTIVFA KDYTTNIRNI FDNKTLTDDF SFYVQNASAS
     DDSLAPAGKS ALYVLVPMPN NDSGLDWQAH CQNVREQVLD TLGARLGLSD IRAHIECEKI
     ITPQTWETDE HVYKGATFSL SHKFSQMLYW RPHNRFEELA NCYLVGGGTH PGSGLPTIYE
     SARISAKLIS QKHRVRFKDI AHSAWLKKAK A