ID   CRTO_STAHJ              Reviewed;         171 AA.
AC   Q4L979;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Glycosyl-4,4'-diaponeurosporenoate acyltransferase;
DE            EC=2.3.1.-;
DE   Flags: Precursor;
GN   Name=crtO; OrderedLocusNames=SH0487;
OS   Staphylococcus haemolyticus (strain JCSC1435).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=279808;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCSC1435;
RX   PubMed=16237012; DOI=10.1128/jb.187.21.7292-7308.2005;
RA   Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y.,
RA   Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C.,
RA   Hiramatsu K.;
RT   "Whole-genome sequencing of Staphylococcus haemolyticus uncovers the
RT   extreme plasticity of its genome and the evolution of human-colonizing
RT   staphylococcal species.";
RL   J. Bacteriol. 187:7292-7308(2005).
CC   -!- FUNCTION: Catalyzes the acylation of glycosyl-4,4'-
CC       diaponeurosporenoate, i.e. the esterification of glucose at the C6''
CC       position with the carboxyl group of the C(15) fatty acid 12-
CC       methyltetradecanoic acid, to yield staphyloxanthin. This is the last
CC       step in the biosynthesis of this orange pigment, present in most
CC       staphylococci strains (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Carotenoid biosynthesis; staphyloxanthin biosynthesis;
CC       staphyloxanthin from farnesyl diphosphate: step 5/5.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the acyltransferase CrtO family. {ECO:0000305}.
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DR   EMBL; AP006716; BAE03796.1; -; Genomic_DNA.
DR   RefSeq; WP_011274812.1; NC_007168.1.
DR   AlphaFoldDB; Q4L979; -.
DR   STRING; 279808.SH0487; -.
DR   EnsemblBacteria; BAE03796; BAE03796; SH0487.
DR   KEGG; sha:SH0487; -.
DR   eggNOG; ENOG503340V; Bacteria.
DR   HOGENOM; CLU_133300_0_0_9; -.
DR   OMA; FNLPCLW; -.
DR   OrthoDB; 1881045at2; -.
DR   UniPathway; UPA00029; UER00560.
DR   Proteomes; UP000000543; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR044021; CrtO.
DR   Pfam; PF18927; CrtO; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Carotenoid biosynthesis; Cell membrane; Membrane; Signal;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..171
FT                   /note="Glycosyl-4,4'-diaponeurosporenoate acyltransferase"
FT                   /id="PRO_0000284856"
FT   TRANSMEM        125..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   171 AA;  21014 MW;  DA24650795825771 CRC64;
     MSLWKVMKLG LIHSAFWTTI QLSVSNMMLR CPTSVFVKYE TFFKIWSWEK RGALWNKLFR
     INKWKHYIPE AAQFNYRIYN KRRLASFKLE DIHFMILEMR RAELVHWLSM IPIVIFIKAP
     KYILFINVCY VISAKLPIIL TQRYNRPRLE HYYQLRMKRD ERTCRKRKSS L