CRTP1_DICDI
ID CRTP1_DICDI Reviewed; 473 AA.
AC Q9GSB0; Q550H3;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=Crt homolog 1;
DE AltName: Full=Chloroquine resistance transporter paralog 1;
DE Short=DdCRTp1;
GN Name=crtp1; Synonyms=ssa662; ORFNames=DDB_G0276943;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=AX4;
RX PubMed=11343215; DOI=10.1086/320707;
RA Nomura T., Carlton J.M.-R., Baird J.K., del Portillo H.A., Fryauff D.J.,
RA Rathore D., Fidock D.A., Su X.-Z., Collins W.E., McCutchan T.F.,
RA Wootton J.C., Wellems T.E.;
RT "Evidence for different mechanisms of chloroquine resistance in 2
RT Plasmodium species that cause human malaria.";
RL J. Infect. Dis. 183:1653-1661(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP IDENTIFICATION.
RX PubMed=15883156; DOI=10.1074/jbc.m503227200;
RA Naude B., Brzostowski J.A., Kimmel A.R., Wellems T.E.;
RT "Dictyostelium discoideum expresses a malaria chloroquine resistance
RT mechanism upon transfection with mutant, but not wild-type, Plasmodium
RT falciparum transporter PfCRT.";
RL J. Biol. Chem. 280:25596-25603(2005).
RN [5]
RP IDENTIFICATION.
RX PubMed=16987557; DOI=10.1016/j.molbiopara.2006.08.006;
RA Sa J.M., Yamamoto M.M., Fernandez-Becerra C., de Azevedo M.F.,
RA Papakrivos J., Naude B., Wellems T.E., Del Portillo H.A.;
RT "Expression and function of pvcrt-o, a Plasmodium vivax ortholog of pfcrt,
RT in Plasmodium falciparum and Dictyostelium discoideum.";
RL Mol. Biochem. Parasitol. 150:219-228(2006).
CC -!- FUNCTION: May regulate endogenous transporter. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CRT-like transporter family. {ECO:0000305}.
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DR EMBL; AF317500; AAG31811.1; -; mRNA.
DR EMBL; AAFI02000019; EAL68973.1; -; Genomic_DNA.
DR RefSeq; XP_642959.1; XM_637867.1.
DR AlphaFoldDB; Q9GSB0; -.
DR SMR; Q9GSB0; -.
DR STRING; 44689.DDB0185065; -.
DR TCDB; 2.A.7.20.2; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; Q9GSB0; -.
DR EnsemblProtists; EAL68973; EAL68973; DDB_G0276943.
DR GeneID; 8620828; -.
DR KEGG; ddi:DDB_G0276943; -.
DR dictyBase; DDB_G0276943; crtp1.
DR eggNOG; ENOG502QR5M; Eukaryota.
DR HOGENOM; CLU_571661_0_0_1; -.
DR InParanoid; Q9GSB0; -.
DR OMA; IWPALMI; -.
DR PhylomeDB; Q9GSB0; -.
DR PRO; PR:Q9GSB0; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0012506; C:vesicle membrane; IDA:dictyBase.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR013936; CRT-like.
DR InterPro; IPR017258; Transprt_Chloroquine.
DR Pfam; PF08627; CRT-like; 1.
DR PIRSF; PIRSF037671; Transprt_Chloroquine_res; 1.
PE 2: Evidence at transcript level;
KW Cytoplasmic vesicle; Glycoprotein; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..473
FT /note="Crt homolog 1"
FT /id="PRO_0000385363"
FT TOPO_DOM 1..49
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 71..80
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..121
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 143..146
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 168..175
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..207
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 229..248
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 270..322
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 344..352
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 374
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 375..395
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 396..473
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 295
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 473 AA; 51809 MW; 7075EE2AD148FFB6 CRC64;
MTNNDKEKQP LLSSINNEDD NGATINIVEP VPWYSNIPQK IKNSMSKETI TILIYVVLYV
TSGVINSVLL KKVMNKFTNY AFFLSQLTNF GYVPIFGAVT AYKIFFTKDI PQETRDFPTR
KFAIMGALDA ITGFFVVIGG VSTSGPLQQL LNQAIIPFTM IASFIFLKER YSLIQLGGAL
VIIGGVVTSL IPSLLGGSSG GNKPFWNFFY LLSVIPGALS NVYKDIGFQA VADMDVWYLQ
YWDSLYQSIF GLFLFPVNNW LPPPATVKFE QILPFMKEGA ECLAGINSII PSYINGTSSF
TATSCTYAPD ATITCDDCHN AWIVIILYMT INIIYNIFIL LVLKHAGATV YSIANTLRLP
LTNIVFSIHF IMGSAVSPFS GLSVAGLVII LVGLGGYRVG SMIKQKKAAA AGGAIKVIPS
YGPAGMDNII PVQKEYIEPK SQAHLRNQFF GKLGINVPEA RYRAANNNNY GDA
