CRTP2_DICDI
ID CRTP2_DICDI Reviewed; 484 AA.
AC Q550A6;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Crt homolog 2;
DE AltName: Full=Chloroquine resistance transporter paralog 2;
DE Short=DdCRTp2;
GN Name=crtp2; ORFNames=DDB_G0277321;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP IDENTIFICATION.
RX PubMed=15883156; DOI=10.1074/jbc.m503227200;
RA Naude B., Brzostowski J.A., Kimmel A.R., Wellems T.E.;
RT "Dictyostelium discoideum expresses a malaria chloroquine resistance
RT mechanism upon transfection with mutant, but not wild-type, Plasmodium
RT falciparum transporter PfCRT.";
RL J. Biol. Chem. 280:25596-25603(2005).
RN [4]
RP IDENTIFICATION.
RX PubMed=16987557; DOI=10.1016/j.molbiopara.2006.08.006;
RA Sa J.M., Yamamoto M.M., Fernandez-Becerra C., de Azevedo M.F.,
RA Papakrivos J., Naude B., Wellems T.E., Del Portillo H.A.;
RT "Expression and function of pvcrt-o, a Plasmodium vivax ortholog of pfcrt,
RT in Plasmodium falciparum and Dictyostelium discoideum.";
RL Mol. Biochem. Parasitol. 150:219-228(2006).
CC -!- FUNCTION: May regulate endogenous transporter. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CRT-like transporter family. {ECO:0000305}.
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DR EMBL; AAFI02000019; EAL68847.1; -; Genomic_DNA.
DR RefSeq; XP_642710.1; XM_637618.1.
DR AlphaFoldDB; Q550A6; -.
DR SMR; Q550A6; -.
DR PaxDb; Q550A6; -.
DR EnsemblProtists; EAL68847; EAL68847; DDB_G0277321.
DR GeneID; 8620902; -.
DR KEGG; ddi:DDB_G0277321; -.
DR dictyBase; DDB_G0277321; crtp2.
DR eggNOG; ENOG502QR5M; Eukaryota.
DR HOGENOM; CLU_571661_0_0_1; -.
DR InParanoid; Q550A6; -.
DR OMA; FTMPLPY; -.
DR PhylomeDB; Q550A6; -.
DR PRO; PR:Q550A6; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR013936; CRT-like.
DR InterPro; IPR017258; Transprt_Chloroquine.
DR Pfam; PF08627; CRT-like; 1.
DR PIRSF; PIRSF037671; Transprt_Chloroquine_res; 1.
PE 3: Inferred from homology;
KW Cytoplasmic vesicle; Glycoprotein; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..484
FT /note="Crt homolog 2"
FT /id="PRO_0000385364"
FT TOPO_DOM 1..57
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 79..94
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 116..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..154
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 176..178
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..210
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 232..244
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 266..328
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 350..355
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 356..378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 379..382
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 383..403
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 404..484
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 206
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 302
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 484 AA; 53676 MW; 8A2EDABB6EE424EB CRC64;
MSEEKLPLLS PLNENDIEND YKDENLKSDL DKLSNVKKQS IIQRFKDYLK NSISKQTATV
LVYVVLYILS GVINSLLLKK VMNVFTNYGF FLNQLTNYGY VPIFGAIVLY KILFTNDIPK
DTRSFPQWKF VIMGALDAVT GYFVVIGGIK TTGPLQQLLN QSVIPFTMLL SFIFLKERYS
LIQLGGALII IGGVVVSLIP SLTGGNTSGN MLFYNFFYLI SMIPYAFSNV YKAIGFSTVE
DMDVWYLQYF DALYQSLVGT VLFPINNWLP PPSDMKFNQV IPQLKAGGKC LGGINTLIEQ
YNSTTGELLP TSCNYGDNLG CDNCHGAWVV VLIYMAVNVL YNVFILLVLK HAGATVFSIA
NTLRLPLTNI AFSFKFIMGS DSNPFSGLSV AGLCIILLGL GGYRVGSMIK QKKEAAASAD
SSSNTENKVI PKVFPTQFGE VSIIIKKKVP PKSQTHLRNQ FFGKLGITVP ESKLRNQNSI
YGDQ