ID   CRTP_STAA8              Reviewed;         497 AA.
AC   Q2FV57;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=4,4'-diaponeurosporene oxygenase {ECO:0000303|PubMed:15933032};
DE            EC=1.14.99.- {ECO:0000305|PubMed:15933032};
DE   AltName: Full=4,4'-diaponeurosporene oxidase {ECO:0000303|PubMed:22535955};
DE   AltName: Full=Carotenoid oxidase {ECO:0000303|PubMed:15933032};
GN   Name=crtP {ECO:0000303|PubMed:22535955};
GN   Synonyms=crtNb {ECO:0000303|PubMed:15933032};
GN   OrderedLocusNames=SAOUHSC_02881;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, AND SUBSTRATE SPECIFICITY.
RC   STRAIN=NCTC 8325 / PS 47;
RX   PubMed=15933032; DOI=10.1128/aem.71.6.3294-3301.2005;
RA   Tao L., Schenzle A., Odom J.M., Cheng Q.;
RT   "Novel carotenoid oxidase involved in biosynthesis of 4,4'-diapolycopene
RT   dialdehyde.";
RL   Appl. Environ. Microbiol. 71:3294-3301(2005).
RN   [3]
RP   FUNCTION, AND PATHWAY.
RC   STRAIN=KCTC 1928;
RX   PubMed=22535955; DOI=10.1074/jbc.m112.343020;
RA   Kim S.H., Lee P.C.;
RT   "Functional expression and extension of staphylococcal staphyloxanthin
RT   biosynthetic pathway in Escherichia coli.";
RL   J. Biol. Chem. 287:21575-21583(2012).
CC   -!- FUNCTION: Involved in the biosynthesis of the yellow-orange carotenoid
CC       staphyloxanthin, which plays a role in the virulence via its protective
CC       function against oxidative stress (PubMed:15933032, PubMed:22535955).
CC       Catalyzes the oxidation of the terminal methyl side group of 4,4'-
CC       diaponeurosporene to form 4,4'-diaponeurosporen-4-al (PubMed:15933032,
CC       PubMed:22535955). {ECO:0000269|PubMed:15933032,
CC       ECO:0000269|PubMed:22535955}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 AH2 + all-trans-4,4'-diaponeurosporene + 2 O2 = 4,4'-
CC         diaponeurosporenal + 2 A + 3 H2O; Xref=Rhea:RHEA:56104,
CC         ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17499, ChEBI:CHEBI:62743, ChEBI:CHEBI:79065;
CC         Evidence={ECO:0000305|PubMed:15933032};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000250|UniProtKB:P21685};
CC   -!- PATHWAY: Carotenoid biosynthesis; staphyloxanthin biosynthesis;
CC       staphyloxanthin from farnesyl diphosphate: step 3/5.
CC       {ECO:0000269|PubMed:22535955}.
CC   -!- MISCELLANEOUS: Upon coexpression with Methylomonas 4,4'-diapophytoene
CC       desaturase (CrtN), CrtP produces 4,4'-diapolycopene dialdehyde, thus
CC       accepting 4,4'-diapolycopene as substrate.
CC       {ECO:0000269|PubMed:15933032}.
CC   -!- SIMILARITY: Belongs to the carotenoid/retinoid oxidoreductase family.
CC       CrtP subfamily. {ECO:0000305}.
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DR   EMBL; CP000253; ABD31878.1; -; Genomic_DNA.
DR   RefSeq; WP_000160456.1; NZ_LS483365.1.
DR   RefSeq; YP_501335.1; NC_007795.1.
DR   AlphaFoldDB; Q2FV57; -.
DR   SMR; Q2FV57; -.
DR   STRING; 1280.SAXN108_2815; -.
DR   EnsemblBacteria; ABD31878; ABD31878; SAOUHSC_02881.
DR   GeneID; 3921551; -.
DR   KEGG; sao:SAOUHSC_02881; -.
DR   PATRIC; fig|93061.5.peg.2604; -.
DR   eggNOG; COG1233; Bacteria.
DR   HOGENOM; CLU_019722_2_1_9; -.
DR   OMA; FTMRWVF; -.
DR   BioCyc; MetaCyc:MON-13879; -.
DR   UniPathway; UPA00029; UER00558.
DR   PRO; PR:Q2FV57; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 2.
DR   InterPro; IPR002937; Amino_oxidase.
DR   InterPro; IPR014105; Carotenoid/retinoid_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   Pfam; PF01593; Amino_oxidase; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR02734; crtI_fam; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; FAD; Flavoprotein; Oxidoreductase;
KW   Reference proteome; Virulence.
FT   CHAIN           1..497
FT                   /note="4,4'-diaponeurosporene oxygenase"
FT                   /id="PRO_0000285231"
FT   BINDING         7..19
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   497 AA;  57187 MW;  20C55EC59459ABF8 CRC64;
     MTKHIIVIGG GLGGISAAIR MAQSGYSVSL YEQNNHIGGK VNRHESDGFG FDLGPSILTM
     PYIFEKLFEY SKKQMSDYVT IKRLPHQWRS FFPDGTTIDL YEGIKETGQH NAILSKQDIE
     ELQNYLNYTR RIDRITEKGY FNYGLDTLSQ IIKFHGPLNA LINYDYVHTM QQAIDKRISN
     PYLRQMLGYF IKYVGSSSYD APAVLSMLFH MQQEQGLWYV EGGIHHLANA LEKLAREEGV
     TIHTGARVDN IKTYQRRVTG VRLDTGEFVK ADYIISNMEV IPTYKYLIHL DTQRLNKLER
     EFEPASSGYV MHLGVACQYP QLAHHNFFFT ENAYLNYQQV FHEKVLPDDP TIYLVNTNKT
     DHTQAPVGYE NIKVLPHIPY IQDQPFTTED YAKFRDKILD KLEKMGLTDL RKHIIYEDVW
     TPEDIEKNYR SNRGAIYGVV ADKKKNKGFK FPKESQYFEN LYFVGGSVNP GGGMPMVTLS
     GQQVADKINA REAKNRK