ID   CRTP_STAAM              Reviewed;         497 AA.
AC   Q99R73;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=4,4'-diaponeurosporene oxygenase {ECO:0000305|PubMed:15850982};
DE            EC=1.14.99.- {ECO:0000305|PubMed:15850982};
DE   AltName: Full=4,4'-diaponeurosporene oxidase {ECO:0000305|PubMed:15850982};
DE   AltName: Full=Carotenoid oxidase {ECO:0000250|UniProtKB:Q2FV57};
DE   AltName: Full=Diapocarotenal synthase {ECO:0000303|PubMed:15850982};
GN   Name=crtP {ECO:0000250|UniProtKB:Q2FV57};
GN   Synonyms=CrtOx {ECO:0000303|PubMed:15850982}; OrderedLocusNames=SAV2564;
OS   Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158878;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu50 / ATCC 700699;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, AND PATHWAY.
RX   PubMed=15850982; DOI=10.1016/j.chembiol.2005.02.010;
RA   Mijts B.N., Lee P.C., Schmidt-Dannert C.;
RT   "Identification of a carotenoid oxygenase synthesizing acyclic
RT   xanthophylls: combinatorial biosynthesis and directed evolution.";
RL   Chem. Biol. 12:453-460(2005).
CC   -!- FUNCTION: Involved in the biosynthesis of the yellow-orange carotenoid
CC       staphyloxanthin, which plays a role in the virulence via its protective
CC       function against oxidative stress. Catalyzes the oxidation of the
CC       terminal methyl side group of 4,4'-diaponeurosporene to form 4,4'-
CC       diaponeurosporen-4-al. The C40 carotenoid lycopene is a poor substrate.
CC       {ECO:0000269|PubMed:15850982}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 AH2 + all-trans-4,4'-diaponeurosporene + 2 O2 = 4,4'-
CC         diaponeurosporenal + 2 A + 3 H2O; Xref=Rhea:RHEA:56104,
CC         ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17499, ChEBI:CHEBI:62743, ChEBI:CHEBI:79065;
CC         Evidence={ECO:0000305|PubMed:15850982};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000250|UniProtKB:P21685};
CC   -!- PATHWAY: Carotenoid biosynthesis; staphyloxanthin biosynthesis;
CC       staphyloxanthin from farnesyl diphosphate: step 3/5.
CC       {ECO:0000305|PubMed:15850982}.
CC   -!- SIMILARITY: Belongs to the carotenoid/retinoid oxidoreductase family.
CC       CrtP subfamily. {ECO:0000305}.
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DR   EMBL; BA000017; BAB58726.1; -; Genomic_DNA.
DR   RefSeq; WP_000160471.1; NC_002758.2.
DR   AlphaFoldDB; Q99R73; -.
DR   SMR; Q99R73; -.
DR   World-2DPAGE; 0002:Q99R73; -.
DR   PaxDb; Q99R73; -.
DR   EnsemblBacteria; BAB58726; BAB58726; SAV2564.
DR   KEGG; sav:SAV2564; -.
DR   HOGENOM; CLU_019722_2_1_9; -.
DR   OMA; FTMRWVF; -.
DR   PhylomeDB; Q99R73; -.
DR   BioCyc; SAUR158878:SAV_RS13985-MON; -.
DR   UniPathway; UPA00029; UER00558.
DR   Proteomes; UP000002481; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 2.
DR   InterPro; IPR002937; Amino_oxidase.
DR   InterPro; IPR014105; Carotenoid/retinoid_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   Pfam; PF01593; Amino_oxidase; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR02734; crtI_fam; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; FAD; Flavoprotein; Oxidoreductase; Virulence.
FT   CHAIN           1..497
FT                   /note="4,4'-diaponeurosporene oxygenase"
FT                   /id="PRO_0000285228"
FT   BINDING         7..19
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   497 AA;  57174 MW;  E20EB9DDF5141C9D CRC64;
     MTKHIIVIGG GLGGISAAIR MAQSGYSVSL YEQNTHIGGK VNRHESDGFG FDLGPSILTM
     PYIFEKLFEY SKKQMSDYVT IKRLPHQWRS FFPDGTTIDL YEGIKETGQH NAILSKQDIE
     ELQNYLNYTR RIDRITEKGY FNYGLDTLSQ IIKFHGPLNA LINYDYVHTM QQAIDKRISN
     PYLRQMLGYF IKYVGSSSYD APAVLSMLFH MQQEQGLWYV EGGIHHLANA LEKLAREEGV
     TIHTGARVDN IKTYQRRVTG VRLDTGEFVK ADYIISNMEV IPTYKYLIHL DTQRLNKLER
     EFEPASSGYV MHLGVACQYP QLAHHNFFFT ENAYLNYQQV FHEKVLPDDP TIYLVNTNKT
     DHTQAPVGYE NIKVLPHIPY IQDQPFTTED YAKFRDKILD KLEKMGLTDL RKHIIYEDVW
     TPEDIEKNYR SNRGAIYGVV ADKKKNKGFK FPKESQYFEN LYFVGGSVNP GGGMPMVTLS
     GQQVADKINA REAKNRK